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  4. Recombinant Escherichia coli CRISPR system Cascade subunit CasA (casA)

Recombinant Escherichia coli CRISPR system Cascade subunit CasA (casA)

  • 中文名称：
    Recombinant Escherichia coli CRISPR system Cascade subunit CasA(casA),Yeast
  • 货号：
    CSB-YP670509ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli CRISPR system Cascade subunit CasA(casA),Yeast
  • 货号：
    CSB-EP670509ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli CRISPR system Cascade subunit CasA(casA),Yeast
  • 货号：
    CSB-EP670509ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Escherichia coli CRISPR system Cascade subunit CasA(casA),Yeast
  • 货号：
    CSB-BP670509ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli CRISPR system Cascade subunit CasA(casA),Yeast
  • 货号：
    CSB-MP670509ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    casA
  • Uniprot No.：
  • 别名：
    casA; cse1; ygcL; b2760; JW2730; CRISPR system Cascade subunit CasA; CRISPR type I-E/Ecoli-associated protein CasA/Cse1; CRISPR-associated protein CasA/Cse1
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-502
  • 氨基酸序列
    MNLLIDNWIP VRPRNGGKVQ IINLQSLYCS RDQWRLSLPR DDMELAALAL LVCIGQIIAP AKDDVEFRHR IMNPLTEDEF QQLIAPWIDM FYLNHAEHPF MQTKGVKAND VTPMEKLLAG VSGATNCAFV NQPGQGEALC GGCTAIALFN QANQAPGFGG GFKSGLRGGT PVTTFVRGID LRSTVLLNVL TLPRLQKQFP NESHTENQPT WIKPIKSNES IPASSIGFVR GLFWQPAHIE LCDPIGIGKC SCCGQESNLR YTGFLKEKFT FTVNGLWPHP HSPCLVTVKK GEVEEKFLAF TTSAPSWTQI SRVVVDKIIQ NENGNRVAAV VNQFRNIAPQ SPLELIMGGY RNNQASILER RHDVLMFNQG WQQYGNVINE IVTVGLGYKT ALRKALYTFA EGFKNKDFKG AGVSVHETAE RHFYRQSELL IPDVLANVNF SQADEVIADL RDKLHQLCEM LFNQSVAPYA HHPKLISTLA LARATLYKHL RELKPQGGPS NG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).; A component of Cascade, which participates in CRISPR interference, the third stage of CRISPR immunity. Cascade binds both crRNA and in a sequence-specific manner negatively supercoiled dsDNA target. This leads to the formation of an R-loop in which the crRNA binds the target DNA, displacing the noncomplementary strand. Cas3 is recruited to Cascade, probably via interactions with CasA, nicks target DNA and then unwinds and cleaves the target, leading to DNA degradation and invader neutralization. CasA is not required for formation of Cascade, but probably enhances binding to and subsequent recognition of both target dsDNA and ssDNA.
  • 基因功能参考文献：
    1. the CasA subunit of Cascade functions as an essential partner of Cas3 by recognizing DNA target sites and positioning Cas3 adjacent to the protospacer asjacent motif to ensure cleavage PMID: 24748111
  • 蛋白家族：
    CRISPR associated protein CasA/Cse1 family, Type I-E/Ecoli subfamily
  • 数据库链接：

    KEGG: ecj:JW2730

    STRING: 316385.ECDH10B_2928