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  4. Recombinant Escherichia coli Alpha-ketoglutarate-dependent taurine dioxygenase (tauD)

Recombinant Escherichia coli Alpha-ketoglutarate-dependent taurine dioxygenase (tauD)

  • 中文名称：
    大肠杆菌tauD重组蛋白
  • 货号：
    CSB-YP336322ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌tauD重组蛋白
  • 货号：
    CSB-EP336322ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌tauD重组蛋白
  • 货号：
    CSB-EP336322ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌tauD重组蛋白
  • 货号：
    CSB-BP336322ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌tauD重组蛋白
  • 货号：
    CSB-MP336322ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tauD
  • Uniprot No.：
  • 别名：
    tauD; ssiD; yaiG; b0368; JW0360; Alpha-ketoglutarate-dependent taurine dioxygenase; EC 1.14.11.17; 2-aminoethanesulfonate dioxygenase; Sulfate starvation-induced protein 3; SSI3
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-283
  • 氨基酸序列
    SERLSITPL GPYIGAQISG ADLTRPLSDN QFEQLYHAVL RHQVVFLRDQ AITPQQQRAL AQRFGELHIH PVYPHAEGVD EIIVLDTHND NPPDNDNWHT DVTFIETPPA GAILAAKELP STGGDTLWTS GIAAYEALSV PFRQLLSGLR AEHDFRKSFP EYKYRKTEEE HQRWREAVAK NPPLLHPVVR THPVSGKQAL FVNEGFTTRI VDVSEKESEA LLSFLFAHIT KPEFQVRWRW QPNDIAIWDN RVTQHYANAD YLPQRRIMHR ATILGDKPFY RAG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the alpha-ketoglutarate-dependent hydroxylation of taurine yielding sulfite and aminoacetaldehyde after decomposition of an unstable intermediate. Is required for the utilization of taurine (2-aminoethanesulfonate) as an alternative sulfur source for growth in the absence of sulfate. To a lesser extent, pentanesulfonate, 3-(N-morpholino)propanesulfonate and 1,3-dioxo-2-isoindolineethanesulfonate are also desulfonated by this enzyme in vitro; however, desulfonation by TauD of organosulfonates other than taurine seem to be of little or no importance for sulfur metabolism in vivo.
  • 基因功能参考文献：
    1. Data suggest that minor active-site perturbations in tauD and alpha-ketoglutarate-dependent nonheme iron oxygenases could allow for changes in substrate reactivity while maintaining substrate triggering and oxygen binding/activation. PMID: 19892731
    2. The structural relationship between substrate taurine and the non-heme Fe(II) center of taurine/alpha-ketoglutarate dioxygenase (TauD) has been defined. PMID: 17469855
  • 蛋白家族：
    TfdA dioxygenase family
  • 数据库链接：

    KEGG: ecj:JW0360

    STRING: 316385.ECDH10B_0324