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  4. Recombinant Drosophila melanogaster Synaptotagmin 1 (Syt1), partial

Recombinant Drosophila melanogaster Synaptotagmin 1 (Syt1), partial

  • 货号：
    CSB-YP324209DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP324209DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP324209DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP324209DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP324209DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Syt1; syt; CG3139; Synaptotagmin 1; p65
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
  • 基因功能参考文献：
    1. This study uncovered essential residues within Synaptotagmin that suggest a structural basis for several activities required for fusion, including a C2B domain surface implicated in SNARE complex interaction that is required for rapid synchronization and Ca(2+) cooperativity of vesicle release. PMID: 28895532
    2. tethering of Syt1 to synaptic vesicles in vivo is a prerequisite for its role in facilitating fast synchronous synaptic vesicle release and suppressing asynchronous and spontaneous fusion PMID: 25775572
    3. synaptic transmission can be regulated by Syt1 multimerization and that both C2 domains of Syt1 are uniquely required for modulating Ca(2+)-independent spontaneous fusion and Ca(2+)-dependent synchronous release. PMID: 23283333
    4. effect of APP gene on synaptotagmin 1 mRNA level PMID: 22419087
    5. The major function of Ca2+ binding to synaptotagmin's C2A domain is to neutralize the negative charge of the pocket, thereby unleashing the fusion-stimulating activity of synaptotagmin. PMID: 22279210
    6. this study provided direct support for the hypothesis that plasma membrane penetration, specifically by the C(2)B domain of synaptotagmin, is the critical effector interaction for coupling Ca(2+) binding with vesicle fusion PMID: 21307261
    7. Results suggest that the tandem C2 domains of Syt 1 play independent roles in neurotransmission. PMID: 20679236
    8. The C(2)B Ca(2+)-binding motif of synaptotagmin is required for synaptic transmission in vivo PMID: 12110842
    9. Synaptotagmins I and IV promote transmitter release independently of Ca(2+) binding in the C(2)A domain PMID: 12110845
    10. Data show that synaptotagmin I is required for a post-docking step during vesicle fusion but does not function to stabilize the docked vesicle state. PMID: 12467585
    11. These results indicate that synaptotagmin is the major Ca(2+) sensor for evoked release and functions to trigger synchronous fusion in response to Ca(2+), while suppressing asynchronous release. PMID: 12467593
    12. Syt I is necessary for the endocytosis of synaptic vesicles that have undergone exocytosis using a functional Syt I protein PMID: 14634669
    13. synaptotagmin 1 is a negative regulator of vesicle fusion of synaptic transmission. PMID: 16061495
    14. Elimination of synaptic transmission and decrease in Ca(2+) affinity of release observed in Ca(2+)-binding motif mutants is not due to altered synaptic vesicle distribution but rather is because of disrupting synaptotagmin I's ability to bind Ca(2+). PMID: 16528727
    15. These results demonstrate that the polylysine motif is required for efficient Ca2+-independent docking and/or priming of synaptic vesicles in vivo. PMID: 16987956
    16. Sr(2+) partially substitutes for Ca(2+) in synchronous release but does not support the negative regulatory function of Syt I. PMID: 17079341
    17. Presence of two presynaptic calcium sensors in a biological context, one a calcium-based sensor devoted primarily to baseline synaptic transmission and a second sensor for short-term synaptic plasticity. PMID: 17709738
    18. human APP gene plays role in synaptogenesis in transgenic lines of Drosophila melanogaster whose nerve cells express the human APP695 isoform, truncated APPs, and the presynaptic marker synaptotagmin driving the sequence of the green fluorescent protein. PMID: 19239106
    19. Results demonstrate that synaptotagmin I plays a direct role in regulating spontaneous transmitter release, indicative of an active role in synaptic vesicle stabilization mediated by the C(2)A polylysine motif. PMID: 19358157

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  • 亚细胞定位：
    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass membrane protein. Cell junction, synapse. Note=Synaptic vesicles in neurons.
  • 蛋白家族：
    Synaptotagmin family
  • 数据库链接：

    KEGG: dme:Dmel_CG3139

    STRING: 7227.FBpp0077410

    UniGene: Dm.19478