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  4. Recombinant Drosophila melanogaster Ras-related protein Rac1 (Rac1)

Recombinant Drosophila melanogaster Ras-related protein Rac1 (Rac1)

  • 货号：
    CSB-YP331223DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP331223DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP331223DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP331223DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP331223DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rac1; RacA; CG2248; Ras-related protein Rac1
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-189
  • 氨基酸序列
    MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDAKP INLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVNPASF ENVRAKWYPE VRHHCPSTPI ILVGTKLDLR DDKNTIEKLR DKKLAPITYP QGLAMAKEIG AVKYLECSAL TQKGLKTVFD EAIRSVLCPV LQPKSKRKC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    During various developmental processes, regulates changes in cell morphology in response to extracellular signals. During oogenesis, mediates signaling from the tyrosine kinase (RTK) chemoattractant receptors (Egfr and Pvr) to the guidance pathway that control the directional persistent collective migration of the border cell (BC) cluster through the nurse cells to the oocyte. Once activating by Pvr and Egfr, promotes the formation of forward-directed actin protrusions which stabilize the DE-cadherin (shg)-mediated adhesions. In turn, DE-mediated adhesion between the leader border cells and nurse cells further promotes Rac1 signaling creating a positive feedback loop that amplifies the output of RTK activity and leads to higher Rac activity at the front, thus promoting polarization of the border cell cluster and directionally persistent migration. Involved in axon outgrowth and myoblast fusion. Plays a role in regulating dorsal closure during embryogenesis. Involved in integrin alpha-PS3/beta-nu-mediated phagocytosis of Gram-positive S.aureus by hemocytes.
  • 基因功能参考文献：
    1. Behavior-evoked activation of Rac1-dependent forgetting has a converging function for autism-risk genes. PMID: 27335463
    2. Rac1, a small GTPase, is specifically required in Eiger-mediated cell death. rac1 loss of function blocks Eiger-induced cell death, whereas Rac1 overexpression enhances Eiger-induced killing. PMID: 27054336
    3. PERK/ATF4 activated the JNK pathway through Rac1 and Slpr activation in apoptotic cells. PMID: 25299777
    4. The small GTPase RAC1 is a driver of intestinal stem cell proliferation through the production of reactive oxygen species. PMID: 23974108
    5. Rac is asymmetrically activated in germline stem cells. PMID: 22802725
    6. These results suggest that Pak3 specifically mediates Rac1 signaling in organizing actin and myosin during Drosophila epidermal wound healing. PMID: 22449966
    7. Apical proteins including beta(H) are negatively regulated by Rac1 activation. PMID: 21111816
    8. Mbc is crucial for the integrity of the F-actin foci and the FCM cytoskeleton, presumably via its activation of Rac1 in these cells. PMID: 21389053
    9. Study reports that a small G protein Rac-dependent forgetting mechanism contributes to both passive memory decay and interference-induced forgetting in Drosophila. PMID: 20178749
    10. Rac1, Rac2 and Mtl, have overlapping functions in the control of epithelial morphogenesis, myoblast fusion, and axon growth and guidance PMID: 11919634
    11. axon growth, guidance and branching could be controlled by differential activation of Rac signalling pathways PMID: 11919635
    12. Drac1 and Crumbs participate in amnioserosa morphogenesis during dorsal closure in Drosophila. PMID: 11973353
    13. Rac1 is partially responsible for mediating the effect of Fmr1 on dendritic development. PMID: 14530299
    14. Both Rac1 and Cdc42 activation induced large growth cones and long filopodia, but Cdc42 did so more efficiently than Rac1. Only Rac1 activation, however, induced thick actin bundles in the filopodia. PMID: 14750960
    15. Rac plays multiple roles in epithelial sheet fusion, including dorsal closure. PMID: 15936337
    16. Rac1 is involved in the regulation of myoblast proliferation and segregation during adult myogenesis. PMID: 16125691
    17. Rac1 induces Bsk activity and stable actin formation for cellular immune activation, leading to sessile hemocyte release and an increase in the number of circulating hemocytes. PMID: 16621891
    18. Reducing activity of the Egfr, Vav, or Rac1 from somatic support cells enhanced the germ cell enclosure defects of a conditional Spitz allele. PMID: 17629483

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side.
  • 蛋白家族：
    Small GTPase superfamily, Rho family
  • 组织特异性：
    Detected in oocyte border cells (BC), with increased expression at the front of the BC cluster.
  • 数据库链接：

    KEGG: dme:Dmel_CG2248

    STRING: 7227.FBpp0304252

    UniGene: Dm.4299