Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Drosophila melanogaster Protein gurken (grk), partial

Recombinant Drosophila melanogaster Protein gurken (grk), partial

  • 中文名称：
    黑腹果蝇grk重组蛋白
  • 货号：
    CSB-YP337029DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇grk重组蛋白
  • 货号：
    CSB-EP337029DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇grk重组蛋白
  • 货号：
    CSB-EP337029DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇grk重组蛋白
  • 货号：
    CSB-BP337029DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇grk重组蛋白
  • 货号：
    CSB-MP337029DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    grk
  • Uniprot No.：
  • 别名：
    grk; CG17610Protein gurken
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Critical for defining the anterior-posterior and dorsal-ventral axes of the egg. May signal directly to dorsal follicle cells through the receptor torpedo (top). During oogenesis this signaling pathway instructs follicle cells to follow a dorsal pathway of development rather than the default ventral pathway.
  • 基因功能参考文献：
    1. sites phosphorylated by CK2 overlap with active Orb and with Gurken protein expression. PMID: 27841697
    2. The study suggests that both the localization and the translation of gurken mRNA in the vitellarium is independent from Vasa. PMID: 28207183
    3. The translational regulation of gurken protein in nurse cells, where the transcript is synthesized, occurs by a different mechanism from that of oskar protein and bicoid protein mRNA in the oocyte. PMID: 26947065
    4. reducing the function of eIF1A in spnB ovaries suppresses the ventralized eggshell phenotype by restoring Grk expression PMID: 25231760
    5. A new pathway is identified that mediates cell-type and branch-specific synaptic repulsion, a novel role for EGFR signaling in synaptic target selection, and an unexpected role for Gurken as a muscle-secreted repulsive ligand. PMID: 22021126
    6. The Syx1A dependent trafficking of Grk protein is required for efficient EGFR signaling during dorsal-ventral patterning. PMID: 23127433
    7. Reduced TOR activity promotes grk translation independent of the canonical Vasa RNA helicase and cap-dependent mechanism. PMID: 22328499
    8. grk, a host gene encoding a crucial axis determinant, is a key component of an mRNP complex that regulates Wolbachia proliferation in Drosophila oogenesis. PMID: 22193955
    9. Data indicate that trapping at the anterodorsal corner requires at least one as-yet-unidentified gurken RLE. PMID: 21103393
    10. pipe repression depends on the TGFalpha-like ligand Gurken, which activates the Drosophila EGF receptor in dorsal follicle cells. PMID: 12050143
    11. mutation in the Drosophila Chk2 homolog (DmChk2/Mnk) suppresses the defects in the translation of gurken mRNA and also the defects in oocyte nuclear morphology PMID: 12361566
    12. Bruno binds specifically to the gurken 3'UTR and that the dorso-ventral defects caused by Bruno over-expression are due to a reduction of Gurken levels in the oocyte, Bruno plays similar roles in translational regulation of gurken and oskar. PMID: 12591598
    13. The two steps of gurken RNA transport occur on distinct arrays of microtubules. Such microtubule networks could provide a mechanism for one motor to transport different cargos to distinct subcellular destinations. PMID: 12636913
    14. localization of gurken mRNA coupled to efficient sorting of Gurken out of the endoplasmic reticulum PMID: 15385627
    15. Star interaction with arrest results in excess Gurken protein, supporting the model that gurken is a target of repression. PMID: 15579696
    16. Production of gurken in the nurse cells is sufficient for axis determination in the Drosophila oocyte. PMID: 15829517
    17. Data show that Cornichon (Cni), but not Cni-related, binds to the extracellular domain of Gurken (Grk), and propose that Cni acts as a cargo receptor recruiting Grk into COPII vesicles. PMID: 16396907
    18. Disrupting Dynein function disperses gurken mRNA homogeneously throughout the cytoplasm, whereas the loss of Squid function converts the sponge bodies into active transport particles. PMID: 17925228
    19. Results show that Gurken travels towards the lateral/posterior of the egg chamber in the absence of Cbl, suggesting that Cbl actively regulates Gurken distribution through promoting endocytosis and subsequent degradation. PMID: 18434418
    20. a mitotic cell cycle checkpoint may impinge on grk expression, much as meiotic checkpoints can alter expression of this gene PMID: 18836312
    21. Glorund is part of a complex containing the hnRNP protein Hrp48 and the splicing factor Half-pint and plays a role in gurken and oskar regulation. PMID: 19013444
    22. Data suggest that that Squid interacts with gurken mRNA in the nurse cell nuclei and, together with other factors, maintains gurken in a translationally silent state during its transport to the dorsal anterior region of the oocyte. PMID: 19100729
    23. Glycosphingolipids are necessary for full activation of the EGFR pathway during oogenesis in a time-dependent manner. PMID: 19144719
    24. Bicaudal C and trailer hitch interact genetically. Mutants of these two genes have shared defects in localization of gurken and other anteriorly-localized mRNAs, as well as altered microtubule organization which may underlie the mRNA localization defects. PMID: 19217894
    25. Germline clones of mutant Sec61beta show normal translocation of Gurken into the ER and transport to the Golgi complex, but further traffic to the plasma membrane is impeded. The defect in plasma membrane traffic is specific for Gurken. PMID: 19226464

    显示更多

    收起更多

  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Note=Associates with the membranous cortex of yolk granules. Transiently inserted into the cell membrane, and then reinternalized during yolk uptake. cni is required for its transport to the oocyte cell membrane.
  • 组织特异性：
    Expressed in nurse cells and oocyte up to oogenesis stage 7. Specifically accumulates in dorsal anterior corner of the oocyte during stages 9/10, at later stages expression is seen as an anterior ring. In stage 10 ovaries, it is concentrated between the o
  • 数据库链接：

    KEGG: dme:Dmel_CG17610

    STRING: 7227.FBpp0079313

    UniGene: Dm.355