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  4. Recombinant Drosophila melanogaster Cofilin/actin-depolymerizing factor homolog (tsr)

Recombinant Drosophila melanogaster Cofilin/actin-depolymerizing factor homolog (tsr)

  • 中文名称：
    黑腹果蝇tsr重组蛋白
  • 货号：
    CSB-YP338237DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇tsr重组蛋白
  • 货号：
    CSB-EP338237DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇tsr重组蛋白
  • 货号：
    CSB-EP338237DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇tsr重组蛋白
  • 货号：
    CSB-BP338237DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇tsr重组蛋白
  • 货号：
    CSB-MP338237DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tsr
  • Uniprot No.：
  • 别名：
    tsr; Cadf; CG4254Cofilin/actin-depolymerizing factor homolog; Protein D61; Protein twinstar
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-148
  • 氨基酸序列
    MASGVTVSDV CKTTYEEIKK DKKHRYVIFY IRDEKQIDVE TVADRNAEYD QFLEDIQKCG PGECRYGLFD FEYMHQCQGT SESSKKQKLF LMSWCPDTAK VKKKMLYSSS FDALKKSLVG VQKYIQATDL SEASREAVEE KLRATDRQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Exhibits F-actin depolymerizing activity and regulates actin cytoskeleton dynamics. Required for cytokinesis in both mitotic and meiotic cells and for aster migration and separation. Promotes cell motility during ovary development and oogenesis. During larval development, required for the cell rearrangement needed for formation of terminal filaments which are stacks of somatic cells that are important for the initiation of ovarioles. Also required for border cell migration during oogenesis. During border cell migration, required for actin turnover and lamellipodial protrusion. Required for the establishment of planar cell polarity (PCP) where cells adopt a uniform orientation within the plane of an epithelium. During establishment of PCP, required for the redistribution of the PCP core proteins fz and stan/fmi to the proximodistal cell boundary. During pupal development, required for elongation of the retinal cell body and for rhabdomere morphogenesis. Required for mushroom body neuroblast proliferation and axon growth. Plays a role in the positive regulation of protein secretion. Plays a role in the regulation of nuclear localization of actin. Required for the maintenance of epithelial integrity by controlling cell junctions and is also necessary for cell survival and tissue growth through regulation of JNK and yki signaling.
  • 基因功能参考文献：
    1. study of the structure, backbone dynamics, and biochemical activities of Twinstar; strong affinity for ADP-G-Actin and ATP-G-Actin; mild F-actin depolymerizing activity and stable interaction with F-actin PMID: 29709602
    2. Taken together, our data suggest that Tsr is required for cell survival and tissue growth by regulating JNK and Yki signaling while maintaining the epithelial integrity by controlling cell junctions. This study provides an insight into potential roles of ADF/cofilin in invasive cell migration and tumor suppression in higher animals. PMID: 27041568
    3. Mical and cofilin, therefore, form a redox-dependent synergistic pair that promotes F-actin instability by rapidly dismantling F-actin and generating post-translationally modified actin that has altered assembly properties PMID: 27454820
    4. Cofilin/Twinstar phosphorylation levels increase in response to impaired coenzyme a metabolism. PMID: 22912811
    5. AIP1 enhances cofilin-mediated actin disassembly in the apical region of precluster cells to promote remodeling of adherense junctions. PMID: 22899846
    6. Data show that cofilin is required for F-actin turnover and lamellipodial protrusion in the border cells. PMID: 21205790
    7. tsr is required for planar cell polarity patterning. PMID: 16571634
    8. These results indicate that changes in the extent of cofilin phosphorylation are regulated by Ssh in response to changes in the levels and/or organisation of F-actin. PMID: 17504806
    9. Results show that twinstar (tsr), which encodes Drosophila cofilin/ADF (actin-depolymerizing factor), is required for elongation of the retinal cell body and the morphogenesis of the rhabdomere. PMID: 18423434

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Nucleus matrix.
  • 蛋白家族：
    Actin-binding proteins ADF family
  • 数据库链接：

    KEGG: dme:Dmel_CG4254

    STRING: 7227.FBpp0072097

    UniGene: Dm.763