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  4. Recombinant Drosophila melanogaster Casein kinase II subunit alpha (CkIIalpha)

Recombinant Drosophila melanogaster Casein kinase II subunit alpha (CkIIalpha)

  • 货号：
    CSB-YP006072DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP006072DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP006072DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP006072DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP006072DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CkIIalpha
  • Uniprot No.：
  • 别名：
    CkIIalpha; Cask-II-a; CG17520Casein kinase II subunit alpha; CK II subunit alpha; EC 2.7.11.1
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-336
  • 氨基酸序列
    TLPSAARVY TDVNAHKPDE YWDYENYVVD WGNQDDYQLV RKLGRGKYSE VFEAINITTT EKCVVKILKP VKKKKIKREI KILENLRGGT NIITLLAVVK DPVSRTPALI FEHVNNTDFK QLYQTLTDYE IRYYLFELLK ALDYCHSMGI MHRDVKPHNV MIDHENRKLR LIDWGLAEFY HPGQEYNVRV ASRYFKGPEL LVDYQMYDYS LDMWSLGCML ASMIFRKEPF FHGHDNYDQL VRIAKVLGTE ELYAYLDKYN IDLDPRFHDI LQRHSRKRWE RFVHSDNQHL VSPEALDFLD KLLRYDHVDR LTAREAMAHP YFLPIVNGQM NPNNQQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site. May participate in Wnt signaling.
  • 基因功能参考文献：
    1. CK2alpha might be important in regulating synaptic plasticity by modulating the transcription of BRP. Hence, we propose that CK2alpha is a novel regulator of the active zone protein, BRP, in Drosophila. PMID: 24080510
    2. overexpression of Tik elicits ectopic bristles in N(+) flies and suppresses the retinal defects of the gain-of-function allele N(spl). PMID: 19536808
    3. Sequencing of full-length cDNA encoding subunits in platelets and megakaryocytic cells PMID: 12102635
    4. CK2 alpha binds and phosphorylates ribosomal protein L22 PMID: 12379220
    5. CK2alpha mutant flies show lengthened circadian period, decreased CK2 activity, and delayed nuclear entry of Per; these effects are probably direct, as CK2alpha specifically phosphorylates Per in vitro PMID: 12447397
    6. casein kinase II has a role in regulating cAMP-response element-binding protein DNA binding by phosphorylation at specific CK sites PMID: 14676200
    7. Dominant-negative CK2alpha induces potent effects on circadian rhythmicity. PMID: 18208335
    8. Results describe the isolation of temperature-sensitive alleles of Drosophila CK2alpha. PMID: 19039653

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  • 亚细胞定位：
    Nucleus, nucleolus.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, CK2 subfamily
  • 数据库链接：

    KEGG: dme:Dmel_CG17520

    STRING: 7227.FBpp0110433

    UniGene: Dm.1750