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  4. Recombinant Drosophila melanogaster 14-3-3 protein epsilon (14-3-3epsilon)

Recombinant Drosophila melanogaster 14-3-3 protein epsilon (14-3-3epsilon)

  • 货号：
    CSB-YP026287DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP026287DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP026287DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP026287DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP026287DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    14-3-3epsilon
  • Uniprot No.：
  • 别名：
    14-3-3epsilon; 14-3-3e; SR3-9; CG3119614-3-3 protein epsilon; Suppressor of Ras1 3-9
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-262
  • 氨基酸序列
    MTERENNVYK AKLAEQAERY DEMVEAMKKV ASMDVELTVE ERNLLSVAYK NVIGARRASW RIITSIEQKE ENKGAEEKLE MIKTYRGQVE KELRDICSDI LNVLEKHLIP CATSGESKVF YYKMKGDYHR YLAEFATGSD RKDAAENSLI AYKAASDIAM NDLPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQAEEV DPNAGDGEPK EQIQDVEDQD VS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Positively regulates Ras-mediated pathways. Acts downstream or parallel to Raf, but upstream of nuclear factors in Ras signaling. Three mutants have been isolated, that suppress the rough eye phenotype caused by mutated Ras1 (sev-Ras1 v12). Inhibits yki activity by restricting its nuclear localization. Together with pav, has a role in the inhibition of microtubule sliding during neurite outgrowth.
  • 基因功能参考文献：
    1. 14-3-3epsilon has a critical role in cellular metabolism involving either molecular crosstalk with the EcR or direct interaction with metabolic proteins PMID: 28365999
    2. role of 14-3-3epsilon in germ cell migration PMID: 22666326
    3. 14-3-3epsilon acts as a biochemical control point for axon guidance in Drosophila, silencing Plexin A repulsive axon guidance and regulating a semaphorin repulsion/integrin adhesion switch. PMID: 22500634
    4. We conclude that 14-3-3epsilon is required for Rab11-positive vesicle function, which in turn enables antimicrobial peptide secretion during an innate immune response. PMID: 21670199
    5. Drosophila 14-3-3/PAR-5 is an essential mediator of PAR-1 function in axis formation. PMID: 12431373
    6. Serves as a central modulator of forkhead transcription factor FoxO activity in the regulation of growth, cell death and longevity in vivo. PMID: 18665908

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  • 蛋白家族：
    14-3-3 family
  • 数据库链接：

    KEGG: dme:Dmel_CG31196

    STRING: 7227.FBpp0082987

    UniGene: Dm.2357