Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Danio rerio Vascular endothelial growth factor A-A (vegfaa)

Recombinant Danio rerio Vascular endothelial growth factor A-A (vegfaa)

  • 货号：
    CSB-YP524826DIL
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP524826DIL
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP524826DIL-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP524826DIL
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP524826DIL
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    vegfaa
  • Uniprot No.：
  • 别名：
    vegfaa; vegf; vegfa; Vascular endothelial growth factor A-A; VEGF-A-A
  • 种属：
    Danio rerio (Zebrafish) (Brachydanio rerio)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    24-188
  • 氨基酸序列
    AHIPKEG GKSKNDVIPF MDVYKKSACK TRELLVDIIQ EYPDEIEHTY IPSCVVLMRC AGCCNDEALE CVPTETRNVT MEVLRVKQRV SQHNFQLSFT EHTKCECRPK AEVKAKKENH CEPCSERRKR LYVQDPLTCK CSCKFTQMQC KSRQLELNER TCRCEKPR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Acts both upstream of kdr and tie1 to stimulate endothelial cell differentiation, and upstream of gata1 to stimulate hematopoietic cell differentiation.
  • 基因功能参考文献：
    1. Vegfaa role in the venous sprouting and spinal cord vascularization. PMID: 28071661
    2. findings identify Vegfa as one of a select few known factors sufficient to activate adult cardiomyogenesis, while also illustrating how instructive factors for heart regeneration require spatiotemporal control for efficacy. PMID: 30104362
    3. Vegfa signaling governs the formation of diverse arteries/veins by distinct cellular mechanisms in vertebrate vasculatures PMID: 29037991
    4. vascular endothelial growth factor and Hedgehog pathways have roles in the development of the superficial system of ocular vessel patterning in zebrafish PMID: 28621424
    5. miR-9 modulation of neuronal VEGF-A controls brain angiogenesis in vivo. PMID: 28813666
    6. Timelapse analysis of individual cardiomyocyte trajectories reveals misdirected cells in zebrafish pdgfra mutants, suggesting that PDGF signaling steers cardiomyocytes toward the midline during cardiac fusion. Intriguingly, the ligand pdgfaa is expressed in the endoderm medial to the pdgfra-expressing myocardial precursors. PMID: 28098558
    7. This study demonstrates the involvement of VEGF signaling in regulating sustained liver injuries after acute alcohol exposure. PMID: 27562099
    8. we determined that radial glia control this process via the Vegf decoy receptor sFlt1: sflt1 mutants exhibit the venous over-sprouting observed in radial glia-ablated larvae, and sFlt1 overexpression rescues it. Genetic mosaic analyses show that sFlt1 function in trunk endothelial cells can limit their over-sprouting. PMID: 27852438
    9. High VEGFA expression is associated with Ectopic Proliferation and Retinal Dysplasia in Zebrafish Optic Pathway Tumors. PMID: 28192065
    10. Rspo1 is required for hematopoietic stem cell specification through control of parallel signaling pathways controlling HSC specification: Wnt16/DeltaC/DeltaD and Vegfa/Tgfbeta1 PMID: 28087636
    11. Tmem2 regulates hyaluronic acid turnover to promote normal Vegf signaling during developmental angiogenesis. PMID: 28118600
    12. low levels of Vegf signaling promote overall vascular endothelial differentiation and cell survival by upregulating etv2 expression, while high levels of Vegf signaling promote arterial and inhibit venous specification. PMID: 28279709
    13. altering the amount of VEGF signaling in endothelial cells by stimulating them with different VEGF concentrations triggered distinct and mutually exclusive dynamic Ca(2+) signaling responses that correlated with different cellular behaviors. PMID: 26905425
    14. Bis(2,3-dibromo-4,5-dihydroxybenzyl) ether downregulate the expression of VEGF and VEGFR, and simultaneously regulates VEGF signaling pathway to prevent angiogenesis. PMID: 26463632
    15. noncanonical function of tars regulates vascular development presumably by modulating the expression of vegfa PMID: 26993167
    16. Methylglyoxal acts on smaller blood vessels in zebrafish via the VEGF receptor signaling cascade, thereby describing a new mechanism that can explain vascular complications under hyperglycemia and elevated MG concentrations. PMID: 25092676
    17. The translation initiation factor eIF3i up-regulates VEGF-A, accelerates cell proliferation, and promotes angiogenesis in embryonic development. PMID: 25147179
    18. lack of SULF1 expression downregulates VEGFA-mediated arterial marker expression, confirming that Sulf1 mediates arterial specification by regulating VegfA165 activity. PMID: 23959107
    19. yolk-derived 17beta-estradiol sets the ventral boundary of hemogenic vascular niche specification by antagonizing the dorsal-ventral regulatory limits of VEGF. PMID: 24871948
    20. Vegfaa has a crucial role in angiogenesis and vegfaa expression was upregulated in embryos lacking functional Pten. PMID: 23720233
    21. Over expression of zebrafish vegf-a rescues Methyl tert-butyl ether induced vascular lesions. PMID: 24128854
    22. Elevated Notch signaling downstream of perturbed VEGF signaling contributes to aberrant flt-1(-/-) blood vessel formation. PMID: 23744993
    23. At the molecular level, we show that the circadian regulator Bmal1 directly targets the promoter region of the vegf gene in zebrafish, leading to an elevated expression of VEGF. PMID: 23324349
    24. Opposing effects of circadian clock genes bmal1 and period2 in regulation of VEGF-dependent angiogenesis in developing zebrafish. PMID: 22884368
    25. findings uncover a novel function for miR-1 and miR-206 in the control of developmental angiogenesis through the regulation of VegfA, and identify a key role for miRNAs as regulators of cross-tissue signaling. PMID: 23132244
    26. Loss of CDS2 results in vascular-specific defects in vivo and failure of VEGFA-induced angiogenesis in endothelial cells in vitro. PMID: 22649102
    27. Hh is able to bypass VEGF to induce arterial differentiation in ECs via the calcitonin receptor-like receptor, thus revealing a surprising complexity in the interplay between Hh and VEGF signaling during arteriovenous specification. PMID: 22668851
    28. Coinjection of low doses of morpholinos for beta-neurexin 1a and neuroligin 1 together or in combination with morpholinos targeting the -heparin--binding isoforms of vascular endothelial growth factor A recapitulates the observed abnormalities PMID: 22516065
    29. sprouting of aortic arch vessels requires a flow-induced genetic pathway in which the mechano-sensitive zinc finger transcription factor klf2a induces expression of an endothelial-specific microRNA, mir-126, to activate Vegf signalling PMID: 20364122
    30. observations reveal a specific requirement for phospholipase C gamma-1 (Plcg1) during formation and differentiation of arteries, and indicate that Vegf is the primary growth factor that utilizes Plcg1 signaling during embryonic development PMID: 12782653
    31. REVIEW: the zebrafish vasculature, zebrafish VEGF and VEGF receptors, advantages of the zebrafish model, resources, and methods of determining growth factor and receptor function PMID: 15522741
    32. an autocrine/paracrine regulation of vegf expression may exist and contribute to the BMP-induced hemangiogenic cell proliferation PMID: 15721310
    33. muscle of the heart uses the VEGF-PLCgamma1 cascade to control the strength of the heart beat PMID: 15998812
    34. In the zebrafish, duplicated VegfA and multiple type III RTKs mediate vascular development. PMID: 17698971
    35. genetic analyses suggest that Vegf/Plcg1 signaling acts at multiple time points and in different signaling contexts to mediate distinct aspects of artery development PMID: 19269286
    36. Results indicate that perlecan coordinates developmental angiogenesis through modulation of VEGF-VEGFR2 signaling events. PMID: 19422911
    37. Results demonstrate that the vascular-specific marker angiomodulin modulates vascular remodeling in part by temporizing the proangiogenic effects of VEGF-A. PMID: 19542015

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    PDGF/VEGF growth factor family
  • 组织特异性：
    Predominantly expressed in regions associated with active vascularization. From 15-16 hours post-fertilization (hpf), expressed in the anterior forebrain, the mesoderm underlying and lateral to the anterior hindbrain, the mesoderm underlying and lateral t
  • 数据库链接：

    KEGG: dre:30682

    STRING: 7955.ENSDARP00000110575

    UniGene: Dr.150231