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  4. Recombinant Danio rerio Sonic hedgehog protein A (shha)

Recombinant Danio rerio Sonic hedgehog protein A (shha)

  • 中文名称：
    斑马鱼shha重组蛋白
  • 货号：
    CSB-YP835612DIL
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    斑马鱼shha重组蛋白
  • 货号：
    CSB-EP835612DIL
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    斑马鱼shha重组蛋白
  • 货号：
    CSB-EP835612DIL-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    斑马鱼shha重组蛋白
  • 货号：
    CSB-BP835612DIL
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    斑马鱼shha重组蛋白
  • 货号：
    CSB-MP835612DIL
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    shha
  • Uniprot No.：
  • 别名：
    shha; shh; vhh1; Sonic hedgehog protein A; SHHA; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; ShhNC; VHH-1) [Cleaved into: Sonic hedgehog protein A N-product; Shh N-terminal processed signaling domains; ShhNp; Sonic hedgehog protein N-product; ShhN)]
  • 种属：
    Danio rerio (Zebrafish) (Brachydanio rerio)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    24-418
  • 氨基酸序列
    CGPGRGY GRRRHPKKLT PLAYKQFIPN VAEKTLGASG RYEGKITRNS ERFKELTPNY NPDIIFKDEE NTGADRLMTQ RCKDKLNSLA ISVMNHWPGV KLRVTEGWDE DGHHFEESLH YEGRAVDITT SDRDKSKYGT LSRLAVEAGF DWVYYESKAH IHCSVKAENS VAAKSGGCFP GSALVSLQDG GQKAVKDLNP GDKVLAADSA GNLVFSDFIM FTDRDSTTRR VFYVIETQEP VEKITLTAAH LLFVLDNSTE DLHTMTAAYA SSVRAGQKVM VVDDSGQLKS VIVQRIYTEE QRGSFAPVTA HGTIVVDRIL ASCYAVIEDQ GLAHLAFAPA RLYYYVSSFL FPQNSSSRSN ATLQQEGVHW YSRLLYQMGT WLLDSNMLHP LGMSVNSS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sonic hedgehog protein: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the reticulum endoplasmic. Once cleaved, ShhC is degraded in the endoplasmic reticulum.; The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites. Involved in the patterning of the anterior-posterior axis of the developing limb bud. Essential for axon guidance. Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO.
  • 基因功能参考文献：
    1. Mutation of hmgcs1 had no effect on Shh signaling at 2 and 3 days post fertilization (dpf), but did result in a decrease in the expression of gli1, a known Shh target gene, at 4 dpf, after morphological deficits in craniofacial development and chondrocyte differentiation were observed in hmgcs1 mutants. PMID: 28686747
    2. Shha/Smo is functionally dedicated to ray branching during fin regeneration. PMID: 28351866
    3. Shh and Rx3 govern formation of a distinct progenitor domain that elaborates patterning through its anisotropic growth and differentiation. PMID: 27317806
    4. Time-lapse imaging revealed that knockdown of miR-219 function accelerates the growth of primary cilia, revealing a possible mechanistic link between miR-219-mediated regulation of apical Par proteins and Shh signaling. PMID: 27226318
    5. Shh is not essential for the early activation of has2, but supports proper chondrogenic differentiation. PMID: 27060628
    6. Opposing Shh and Fgf signals initiate nasotemporal patterning of the zebrafish retina. PMID: 26428010
    7. Hedgehog signaling has a role in dental papilla formation and tooth size during zebrafish odontogenesis PMID: 25645398
    8. Data indicate that the transgenic lines report Hedgehog pathway state in individual cells and with high sensitivity. PMID: 25068273
    9. We further demonstrate that the elevated Hedgehog signaling in Sox11-deficient zebrafish was caused by a large increase in shha transcription; indeed, suppressing Shha expression rescued the ocular phenotypes of sox11 morphants. PMID: 25010521
    10. Pax6 has an evolutionarily conserved function in establishing the temporospatial expression of Shh in the mid-diencephalic organizer in vertebrates. PMID: 24528677
    11. Sulf1 triggers Shh signaling activity to establish and, later on, modify the spatial arrangement of gene expression in ventral neural progenitors PMID: 24595292
    12. These studies provide evidence that a signaling pathway involving agrin, Fgfs and Shh may be a critical target of ethanol exposure during zebrafish embryogenesis. PMID: 23184466
    13. heterozygote mutations in fgf8, shh or oep lead to a reduced number of ascending dopaminergic neurons in zebrafish and may confer increased susceptibility to the Parkinson disease PMID: 23123778
    14. Hh is able to bypass VEGF to induce arterial differentiation in ECs via the calcitonin receptor-like receptor, thus revealing a surprising complexity in the interplay between Hh and VEGF signaling during arteriovenous specification. PMID: 22668851
    15. analysis of Hh signaling which induces pancreatic fibrosis through paracrine activation of Hh-responsive cells in vivo PMID: 22164219
    16. The present study investigates the role of the shha-expressing cells in the patterning of fin ray branches. PMID: 22445510
    17. The Sphingosine-1-phospate-dependent anterior foregut endoderm functions primarily through Shh to regulate the growth but not dorsoventral patterning of zebrafish jaw precursors. PMID: 22185793
    18. function for Bhmt involving modulation of Shh signaling to control beta-cell development. PMID: 21952238
    19. Hedgehog and retinoic acid signaling cooperate to promote motoneurogenesis in zebrafish. PMID: 22069185
    20. Microphthalmia produced by combined agrin MO and ethanol treatment was rescued by sonic hedgehog (Shh) mRNA overexpression. PMID: 21308976
    21. role of Shh signalling in patterning the pharyngeal pouches and in restricting the expression of the parathyroid marker Gcm2 in the pharyngeal epithelium PMID: 21349263
    22. Data show that inhibiting or eliminating hedgehog (Hh) signaling reduces neural progenitor cell proliferation affecting neurogenesis in the optic tectum. PMID: 21219478
    23. IP6K2 is a positive regulator of Hh signaling. PMID: 20980661
    24. Hh signaling is indirectly required via slow fiber specification for recovery of fast fiber elongation in laminin gamma1 mutant embryos PMID: 20063418
    25. fish-specific duplicated dmrt2b contributes to a divergent function in somitogenesis through Hedgehog pathway and maintains the common function for left-right asymmetry establishment PMID: 19789708
    26. There exists cooperation between Nodal and Hedgehog pathways in the maintenance of the anterior-dorsal hypothalamus. PMID: 12070082
    27. Involved in anteroposterior patterning of the zebrafish otic vesicle. PMID: 12588855
    28. roles of signaling in pituitary development PMID: 12606279
    29. role in adenohypophysis formation PMID: 12606280
    30. extraretinal and retinal hedgehog signaling sequentially regulate retinal differentiation PMID: 12798293
    31. Our results do not support the previously suggested dominant roles for sonic hedgehog and Fgf8 in specification of the first catecholaminergic neurons, but instead indicate a novel role for Nodal signaling in this process PMID: 12843251
    32. Disp1 activity is essential for the secretion of lipid-modified Hh proteins from midline structures. PMID: 15110707
    33. Igu/Dzip1 functions as a permissive factor that is required for the proper regulation of Hh target genes in response to Hh signals. PMID: 15115751
    34. hdac1-deficient embryos exhibit several defects of neuronal specification and patterning, including a dramatic deficit of hedgehog-dependent branchiomotor neurones that is refractory to elevated levels of hedgehog signalling. PMID: 15169759
    35. Directs differentiation of inner and outer nuclear layers of retina, also lamination of retina. PMID: 15253932
    36. Shh expression was studied in developing zebrafish. PMID: 15272389
    37. Some multipotent muscle precursors' commitment to the slow fate depends on Hedgehog. PMID: 15464578
    38. spatial and temporal differences in Hh signaling within a common population of neural precursors can contribute to cell fate diversification PMID: 15539490
    39. gli2b expression is affected in Hedgehog and Notch signaling related mutants during zebrafish embryonic development PMID: 15614761
    40. results suggest that a functional interaction between beta-arrestin 2 and Smoothened may be critical to regulate hedgehog signaling in zebrafish development PMID: 15618520
    41. Shh directs cell-cycle exit by activating p57Kip2 in the developing zebrafish retina. PMID: 15891769
    42. results suggest a novel role for Shh in the movements of neural crest cells at the midline, as well as in their differentiation into cartilage PMID: 16049113
    43. Expression of IHH and downstream targets correlates with pit cells. IHH and SMO may be useful biomarkers of diffuse cancers that may show growth inhibition with Hh antagonists such as cyclopamine. PMID: 16831586
    44. Zebra fish foxl1 is a novel regulator of neural development that acts by suppressing shh expression. PMID: 16980626
    45. pattern of shh expression in the embryonic central nervous system involves an intricate crosstalk of at least 4 different regulatory regions PMID: 17157288
    46. Results suggest that Hedgehog proteins can regulate skeletal appendage outgrowth and demonstrates an unexpected mechanism for mediating Shh signals in a median fin primordium. PMID: 17597528
    47. Data show that endoderm and hedgehog signaling, but not Hand2, regulate GDNF expression in the intestine, highlighting a central role of endoderm and Sonic hedgehog in patterning the intestine and the enteric nervous system. PMID: 18436202
    48. Expression of multiple slow myosin heavy chain genes reveals a diversity of zebrafish slow muscle twitch fibers with differing requirements for shh and Prdm1 activity. PMID: 18480160
    49. These results identify Six3 as a direct regulator of Shh expression and reveal a crossregulatory loop between Shh and Six3 in the ventral forebrain. PMID: 18694563
    50. addition of exogenous Shh to ethanol treated zebrafish prevented many of the gross physical phenotypes, suggesting that the suppression of Shh-s is one of the major effects of ethanol exposure. PMID: 19235835

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  • 亚细胞定位：
    Endoplasmic reticulum membrane. Golgi apparatus membrane.; [Sonic hedgehog protein A N-product]: Cell membrane; Lipid-anchor.
  • 蛋白家族：
    Hedgehog family
  • 组织特异性：
    Expressed in the ventral midline of the neural tube and brain. Also found in the notochord and in developing fin bud. In the developing brain, expression occurs in domains that include a discrete region in the floor of the diencephalon.
  • 数据库链接：

    KEGG: dre:30269

    STRING: 7955.ENSDARP00000125090

    UniGene: Dr.115833