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  4. Recombinant Danio rerio Protein naked cuticle homolog 1 (nkd1)

Recombinant Danio rerio Protein naked cuticle homolog 1 (nkd1)

  • 货号：
    CSB-YP648794DIL
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP648794DIL
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP648794DIL-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP648794DIL
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP648794DIL
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    nkd1; Protein naked cuticle homolog 1; Naked-1
  • 种属：
    Danio rerio (Zebrafish) (Brachydanio rerio)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-440
  • 氨基酸序列
    GKLHSKHAA ICKARESPEG DSFVVNACLA RKGLDDWMVK QKYYCSSSRV DQRDCQQKNN CELSPRDPLD EGYAEGISDE HYRLEVALPP EKTDSCSVEE RMQGTEGSAT VGPHKQLQFE ELECAVSVEE DNRQEWTFTL YDFDNNGKVT REDITSLLHT IYEVVDASVN HSPSSSKTLR VKLSVAPDSS QRWKNCTQTN TDTPNPKHKG EKCIEDSKTS EKKSRAFLRR YHAEHHSQQG CQRHCVDENL ERRNHYLDLA GIENYTSRFG TAAPATEPAK PTHATRSSNQ SRSRSQEPEN GQVHPAHHRR SQTMSTDPPA ILTRHTHALR SPKTHRTPPT QASAGRVMRR GAPPPPSVPN QTPPHQSSGP YRRHKQRPKE ALHYRALGPS VTSGPVLEKE HVRDLPSLVL YEGGLAQVVQ RHEHHHHHEH HHHYHHFYQS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity.
  • 基因功能参考文献：
    1. This work reveals that the zebrafish Nkd1 EF-hand is essential to balance Wnt signaling inputs and modulate the appropriate outputs, while the Drosophila-like EF-Hand primarily functions in beta-catenin signaling. PMID: 29180104
    2. Nkd1 activity is specifically dependent on Wnt ligand activation of the receptor. Nkd1 is recruited to the Wnt signalosome with Dvl2, where it becomes activated to move into the cytoplasm to interact with beta-catenin, inhibiting its nuclear accumulation. PMID: 25904337
    3. Nkd1 functions as a passive antagonist of Wnt signaling, functioning only when homeostatic levels of Wnt signaling have been breached or when Wnt signaling becomes destabilized. PMID: 24009776
    4. Data demonstrate that Nkd1 binds to beta-catenin and prevents its nuclear accumulation. Nkd1 function is dependent on its interaction with the cell membrane. PMID: 21490931
    5. Nkd1 knockdown in the dorsal forerunner cells causes beta-catenin nuclear localization/transcriptional activation & changes in DFC migration, Kupffer's vesicle formation, ciliogenesis, left-right patterning, & asymmetric charon expression. PMID: 20858476
    6. data indicate that zebrafish Nkd1 and Nkd2 function to limit both canonical and non-canonical Wnt signaling PMID: 17689523

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  • 亚细胞定位：
    Cell membrane. Cytoplasm.
  • 蛋白家族：
    NKD family
  • 数据库链接：

    KEGG: dre:751108

    STRING: 7955.ENSDARP00000052378

    UniGene: Dr.135817