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  4. Recombinant Danio rerio Protein Wnt-1 (wnt1)

Recombinant Danio rerio Protein Wnt-1 (wnt1)

  • 货号：
    CSB-YP328457DIL
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP328457DIL
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP328457DIL-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP328457DIL
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP328457DIL
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    wnt1; int-1; wnt-1; Protein Wnt-1
  • 种属：
    Danio rerio (Zebrafish) (Brachydanio rerio)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    20-370
  • 氨基酸序列
    S LTGTGAVNNS GRWWGIVNVA SSGNLLTNSK NVQLVLDPSL ALLSRRQRKL IRQNPGILHA IAAGLHTAIK ECKWQFRNRR WNCPTTHSPN VFGKIVNRGC RETAFVFAIT SAGVTHAVAR SCSEGAIESC TCDYRRRGPG GPDWHWGGCS DNVEFGRMFG REFVDSSERG RDLRYLTNLH NNEAGRMTVA SEMQQECKCH GMSGSCTVRT CWMRLPSFRL VGDYLKDRFD GASRVVYANK GSNRASHRAD PRHLEPENPA HKLPSSRDLV YFEKSPNFCS YNGKTGTHGT SGRTCNSSSP ALDGCELLCC GRGYKTRMEQ VTERCHCTFH WCCHVSCLNC TSTQTVHQCL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ligand for members of the frizzled family of seven transmembrane receptors. Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation. Involved in neurogenesis. Performs a partially redundant function with wnt10b in the formation of the midbrain-hindbrain boundary (MHB) organizer. In the hindbrain, mediates lateral inhibition of boundary cell specification, probably via up-regulation of proneural and Delta gene expression in non-boundary cells; localized expression of wnt1 in boundary cells is maintained via rfng-mediated modulation of Notch activity.
  • 基因功能参考文献：
    1. analysis of differential role of Axin RGS domain function in Wnt signaling during anteroposterior patterning and maternal axis formation PMID: 22957046
    2. wnt1 and wnt10b are required to maintain threshold levels of Pax2.1 and Fgf8 at the midbrain-hindbrain boundary. PMID: 12591239
    3. novel role for Wnt/beta-catenin signalling in determining endocardial cell fate PMID: 14534590
    4. In zebrafish embryos lacking Wnt3a, Wnt1 and Wnt10b, the expression of engrailed orthologs, pax2a and fgf8 is not maintained after mid-somitogenesis PMID: 15147762
    5. two Dvl-associated paralogs, Dpr1 and Dpr2, participate in distinct Wnt-dependent developmental processes PMID: 15539487
    6. Wnt/Axin/beta-catenin pathway has a role in ventral CNS development PMID: 15539488
    7. The boundary and roof plate expression of wnt1 each contribute to upregulation of proneural and delta gene expression and neurogenesis in non-boundary regions. PMID: 15659486
    8. Epistatic analyses suggest a possible genetic interaction between Wnt/beta-catenin and Myostatin in regulation of slow and fast twitch muscle myofibrillogenesis PMID: 19517013

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Wnt family
  • 组织特异性：
    In the embryo, expressed in the developing central nervous system (CNS), with an expression pattern very similar to that of wnt10b. During somitogenesis, expressed strongly in hindbrain boundary and roof plate cells.
  • 数据库链接：

    KEGG: dre:30128

    STRING: 7955.ENSDARP00000110057

    UniGene: Dr.85371