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  4. Recombinant Danio rerio Glyceraldehyde-3-phosphate dehydrogenase (gapdh)

Recombinant Danio rerio Glyceraldehyde-3-phosphate dehydrogenase (gapdh)

  • 货号：
    CSB-YP009232DIL
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009232DIL
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009232DIL-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009232DIL
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009232DIL
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    gapdhGlyceraldehyde-3-phosphate dehydrogenase; GAPDH; EC 1.2.1.12; Peptidyl-cysteine S-nitrosylase GAPDH; EC 2.6.99.-
  • 种属：
    Danio rerio (Zebrafish) (Brachydanio rerio)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-333
  • 氨基酸序列
    MVKVGINGFG RIGRLVTRAA FLTKKVEIVA INDPFIDLDY MVYMFQYDST HGKYKGEVKA EGGKLVIDGH AITVYSERDP ANIKWGDAGA TYVVESTGVF TTIEKASAHI KGGAKRVIIS APSADAPMFV MGVNHEKYDN SLTVVSNASC TTNCLAPLAK VINDNFVIVE GLMSTVHAIT ATQKTVDGPS GKLWRDGRGA SQNIIPASTG AAKAVGKVIP ELNGKLTGMA FRVPTPNVSV VDLTVRLEKP AKYDEIKKVV KAAADGPMKG ILGYTEHQVV STDFNGDCRS SIFDAGAGIA LNDHFVKLVT WYDNEFGYSN RVCDLMAHMA SKE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
  • 基因功能参考文献：
    1. amino acid sequences were used to establish the 3D structures of the active site of glyceraldehyde-3-phosphate dehydrogenase and the phylogenetic relationships of the sardine and octopus enzymes PMID: 21106768
    2. The expression level of GAPDH was upregulated to a greater extent than those for myogenin and myostatin 1 under restricted feeding. PMID: 19135165
  • 亚细胞定位：
    Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Nucleus.
  • 蛋白家族：
    Glyceraldehyde-3-phosphate dehydrogenase family
  • 数据库链接：

    KEGG: dre:317743

    STRING: 7955.ENSDARP00000063799

    UniGene: Dr.35640