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  4. Recombinant Danio rerio Cellular tumor antigen p53 (tp53)

Recombinant Danio rerio Cellular tumor antigen p53 (tp53)

  • 货号：
    CSB-YP024077DIL
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP024077DIL
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP024077DIL-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP024077DIL
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP024077DIL
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    tp53; drp53; Cellular tumor antigen p53; Tumor suppressor p53
  • 种属：
    Danio rerio (Zebrafish) (Brachydanio rerio)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-373
  • 氨基酸序列
    MAQNDSQEFA ELWEKNLIIQ PPGGGSCWDI INDEEYLPGS FDPNFFENVL EEQPQPSTLP PTSTVPETSD YPGDHGFRLR FPQSGTAKSV TCTYSPDLNK LFCQLAKTCP VQMVVDVAPP QGSVVRATAI YKKSEHVAEV VRRCPHHERT PDGDNLAPAG HLIRVEGNQR ANYREDNITL RHSVFVPYEA PQLGAEWTTV LLNYMCNSSC MGGMNRRPIL TIITLETQEG QLLGRRSFEV RVCACPGRDR KTEESNFKKD QETKTMAKTT TGTKRSLVKE SSSATLRPEG SKKAKGSSSD EEIFTLQVRG RERYEILKKL NDSLELSDVV PASDAEKYRQ KFMTKNKKEN RESSEPKQGK KLMVKDEGRS DSD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of bax and fas antigen expression, or by repression of Bcl-2 expression.
  • 基因功能参考文献：
    1. Nkx2.5 signaling via activation of Nkx2.5-Calr-p53 signaling pathway results in cardiac dysfunction and hyperglycemia-induced cardiomyopathy. PMID: 28801532
    2. In the hsf4(null) fish, both p53 and activated-caspase3 were significantly decreased. Combined with the finding that the denucleation defect could be partially rescued through microinjection of p53, fas and bax mRNA into the mutant embryos, we directly proved that HSF4 promotes lens fiber cell differentiation by activating p53 and its downstream regulators PMID: 28981088
    3. p53-target genes in Danio rerio have been identified. PMID: 27581768
    4. Data indicate that genetic inhibition of autophagy promotes tumorigenesis in tumor protein p53 (tp53) mutant. PMID: 27655644
    5. Data suggest that the sensitivity of p53 to expression of an editing-defective tRNA synthetase has a critical role in promoting genome integrity and organismal homeostasis. PMID: 27402763
    6. Transcriptome analysis revealed that SepH deficiency induces an inflammatory response and activates the p53 pathway. Consequently, loss of seph renders larvae susceptible to oxidative stress and DNA damage. Finally, we demonstrate that seph interacts with p53 deficiency in adulthood to accelerate gastrointestinal tumor development PMID: 27588899
    7. results suggest that trim69 participates in tp53-mediated apoptosis during zebrafish development. PMID: 27031046
    8. mutant Mdm2 was unable to rescue a p53-induced apoptotic phenotype. PMID: 28846075
    9. C/ebpalpha plays a role in liver growth regulation via the p53 pathway. PMID: 26511037
    10. Results demonstrate a novel role of Klf8, achieved via modulation of p53 and met expression, in the maintenance neuronal progenitors and development of Purkinje cells and the proliferation of granule cells in the cerebella of zebrafish embryos PMID: 25528982
    11. p53 has a limited role in eliciting the anemia phenotype of zebrafish models of Diamond-Blackfan anemia. PMID: 26132763
    12. p53 protein accumulates during tumor formation as a result of tumor-specific inactivation of the Mdm2 pathway. PMID: 25746004
    13. Delta113p53/Delta133p53 is an evolutionally conserved pro-survival factor for DNA damage stress by preventing apoptosis and promoting DNA double-strand break repair to inhibit cell senescence. PMID: 25698579
    14. Apoptosis was also observed with myca expression; introduction of homozygous tp53(-/-) mutation into the myca transgenic fish reduced apoptosis and accelerated tumor progression. PMID: 25612309
    15. The crystal structure of the zebrafish p53 tetramerization domain. PMID: 25185827
    16. The identification of novel p53 isoforms with anti-apoptosis function generated due to alternative splicing of intron 8 of p53. PMID: 25550427
    17. L-Leucine thus alleviates anaemia in RP-deficient cells in a TP53-independent manner. PMID: 25098371
    18. By the loss-of-function of p53. PMID: 24801718
    19. Data suggest that the co-inhibition of Tp53 activity rescued the morphological deformities but did not alleviate the erythroid aplasia indicating that ribosomal protein deficiency causes erythroid failure in a Tp53-independent manner. PMID: 24417973
    20. Carcinogenesis in zebrafish with combined mutations in tp53 and brca2 typically requires biallelic mutation or loss of at least one of these genes. PMID: 24489863
    21. tp53 mutations have no significant influence on the onset of myc-induced T-ALL in zebrafish PMID: 24690081
    22. protein-protein interaction between Delta113p53 and p53 is essential for the anti-apoptotic function of Delta113p53 PMID: 24576456
    23. HBx and src overexpression induced hepatocarcinogenesis in p53 mutant zebrafish PMID: 24130815
    24. In addition, they unravel essential roles of TAp63 and p53 to promote both keratinocyte proliferation and their terminal differentiation by promoting Notch signalling and caspase 3 activity. PMID: 24415949
    25. These results suggest a role for mitochondrial p53 activity in promoting hair cell death due to aminoglycosides, likely upstream of Bax and Bcl2. PMID: 23821348
    26. Data indicate that co-inhibition of p53 partially alleviates the abnormalities for RPS19-deficient embryos. PMID: 23990987
    27. Zebrafish gene expressions of P53, Bcl-2, Bax and caspase-3 were elevated after exposure with microcystin-LR under different ambient temperatures. PMID: 22407967
    28. Data indicate that knockdown of the Mdm2 and Mdm4 caused dramatic accumulation of mutant p53 protein. PMID: 23069659
    29. Eif3ba has a role in regulating cranial neural crest development by modulating p53 in zebrafish PMID: 23791820
    30. This is the first report using an animal model to show interactions between tsc2, mTORC1 and p53 during tumorigenesis. PMID: 23580196
    31. Targeted gene knockdown of TNFRSF1B in zebrafish embryos results in the induction of a caspase-8, caspase-2 and P53-dependent apoptotic program in endothelial cells that bypasses caspase-3. PMID: 22956347
    32. In our zebrafish model, autophagy induction does not depend on inhibition of the Tor pathway or activation of Tp53. PMID: 23408911
    33. the exaptation of lineage-specific transposons to shape p53-regulated neuronal morphogenesis-related pathways in both a hominid and a teleost fish. PMID: 23118857
    34. p53 is involved in apoptosis upon Aurora-A depletion in zebrafish embryogenesis. PMID: 23351126
    35. The stabilities of the full-length p53 orthologs were marginal and correlated with the temperature of their organism, paralleling the stability of the isolated DNA-binding domains. PMID: 23112865
    36. Ccdc94 and the Prp19 complex protect cells from IR-induced apoptosis by repressing the expression of p53 mRNA PMID: 22952453
    37. Deficiency in dkc1 and nola1 in the H/ACA RNP complex likely contributes to the hematopoietic phenotype through p53 activation associated with rRNA processing defects during the initial stage of Dyskeratosis congenita pathogenesis. PMID: 22299032
    38. zebrafish Mms2 and Ubc13 form a stable complex, which is required for p53-mediated DNA-damage response PMID: 22055568
    39. p53 mediates the cellular defects associated with rps29. rps29 & p53 play roles in hematopoietic stem cells & red blood cell development. PMID: 22120640
    40. Our data support a model of DC whereupon in early development mutations involved in the H/ACA complex contribute to bone marrow failure through p53 deregulation. PMID: 21921046
    41. p53 protected against hypoxia-induced cell death mediated by p53 suppression of bnip3 as illustrated by p53 knockdown/loss assays in a zebrafish model. PMID: 21792176
    42. p53 upregulation is a frequent response to deficiency of cell-essential genes PMID: 21209837
    43. Shh-mediated control of p53 activity is required for proliferation and cell cycle exit of retinal cells as well as differentiation of amacrine cells and photoreceptors PMID: 21042410
    44. Results describe the functional aspects of the zebrafish DeltaNp53 ortholog in the context of FLp53 and the zebrafish Delta133p53 ortholog (Delta113p53) coexpressed in the developing embryo. PMID: 20929554
    45. Tp53-mediated germ cell apoptosis induces sex reversal after the mutation of a DNA-repair pathway gene by compromising the survival of oocytes PMID: 20661450
    46. Cloned and expressed p53 protein from zebrafish in Escherichia coli. RT-PCR analysis confirmed that the purified p53 protein could bind specifically to its cognate mRNA. PMID: 20363330
    47. Real-time RT-PCR revealed acetaminophen treatment of zebrafish embryos decreased the expression of cox2 and bcl2, but increased p53 expression. PMID: 20170747
    48. summarized the recent studies on the p53 isoform Delta113p53 regarding its regulation and function in zebrafish. [Review] PMID: 19916829
    49. UV treatment promotes phosphorylation of H2AX and a p53-dependent response. PMID: 20047468
    50. p53 deficiency mediated suppression of leukemic differentiation in C3H/He myeloid leukemia PMID: 12443880

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    P53 family
  • 数据库链接：

    KEGG: dre:30590

    STRING: 7955.ENSDARP00000051548

    UniGene: Dr.75100