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  4. Recombinant Cysteine protease yopT (yopT)

Recombinant Cysteine protease yopT (yopT)

  • 货号：
    CSB-YP527859YAS
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP527859YAS
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP527859YAS-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP527859YAS
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP527859YAS
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    yopT
  • Uniprot No.：
  • 别名：
    yopT; YPCD1.20; y5059; y0065; YP_pCD67Cysteine protease YopT; EC 3.4.22.-
  • 种属：
    Yersinia pestis
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-322
  • 氨基酸序列
    MNSIHGHYHI QLSNYSAGEN LQSATLTEGV IGAHRVKVET ALSHSNLQKK LSATIKHNQS GRSMLDRKLT SDGKANQRSS FTFSMIMYRM IHFVLSTRVP AVRESVANYG GNINFKFAQT KGAFLHKIIK HSDTASGVCE ALCAHWIRSH AQGQSLFDQL YVGGRKGKFQ IDTLYSIKQL QIDGCKADVD QDEVTLDWFK KNGISERMIE RHCLLRPVDV TGTTESEGLD QLLNAILDTH GIGYGYKKIH LSGQMSAHAI AAYVNEKSGV TFFDPNFGEF HFSDKEKFRK WFTNSFWGNS MYHYPLGVGQ RFRVLTFDSK EV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cysteine protease, which is translocated into infected cells and plays a central role in pathogenesis by cleaving the C-terminus end of the human small GTPase RhoA/ARHA, a regulator of cytoskeleton. Once cleaved, ARHA loses its lipid modification, and is released from the cell membrane, leading to the subsequent disruption of actin cytoskeleton of the host cell.
  • 基因功能参考文献：
    1. Computational analyses revealed that the N-terminal region is predicted to be mostly disordered. In the full-length YopT isoforms, the disordered N-terminal tail contains a disorder-based potential binding site. PMID: 26845766
  • 亚细胞定位：
    Secreted. Note=In infected cells, it is cytoplasmic. Translocated into the host cell by the type III secretion apparatus with the help of the SycT chaperone.
  • 蛋白家族：
    Peptidase C58 family
  • 数据库链接：

    KEGG: ype:YPCD1.20