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  4. Recombinant Crotalus adamanteus L-amino-acid oxidase

Recombinant Crotalus adamanteus L-amino-acid oxidase

  • 货号：
    CSB-YP011660DYB
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP011660DYB
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP011660DYB-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP011660DYB
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP011660DYB
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    L-amino-acid oxidase; LAAO; LAO; EC 1.4.3.2
  • 种属：
    Crotalus adamanteus (Eastern diamondback rattlesnake)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    19-516
  • 氨基酸序列
    AH DRNPLEECFR ETDYEEFLEI AKNGLTATSN PKRVVIVGAG MAGLSAAYVL AGAGHQVTVL EASERVGGRV RTYRKKDWYA NLGPMRLPTK HRIVREYIKK FDLKLNEFSQ ENENAWYFIK NIRKRVREVK NNPGLLEYPV KPSEEGKSAA QLYVESLRKV VEELRSTNCK YILDKYDTYS TKEYLLKEGN LSPGAVDMIG DLLNEDSGYY VSFIESLKHD DIFGYEKRFD EIVGGMDQLP TSMYEAIKEK VQVHFNARVI EIQQNDREAT VTYQTSANEM SSVTADYVIV CTTSRAARRI KFEPPLPPKK AHALRSVHYR SGTKIFLTCT KKFWEDDGIH GGKSTTDLPS RFIYYPNHNF TSGVGVIIAY GIGDDANFFQ ALDFKDCADI VINDLSLIHE LPKEDIQTFC HPSMIQRWSL DKYAMGGITT FTPYQFQHFS EALTAPFKRI YFAGEYTAQF HGWIDSTIKS GLTAARDVNR ASENPSGIHL SNDNEF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Please could you let me know whether recombinant Crotalus adamanteus L-amino-acid oxidase from Escherichia Coli, product code
CSB-EP011660DYB, has been tested for enzyme activity. If not have any on the other sources of this recombinant enzyme from Crotalus adamanteus been tested? Also the cost of 1mg of activity tested enzyme and the likely lead time to delivery.

A:
Thanks for your inquiry on our product CSB-EP011660DYB. http://www.cusabio.com/Recombinant-Protein/Recombinant-Crotalus-adamanteus--L-amino-acid-oxidase-Escherichia-Coli-11268570.html
I'm afraid that the enzyme activity of CSB-EP011660DYB haven't been test. We have this protein in other sources, but the activity also haven't been test.
This protein is the mature full length protein, and we purified under the mild conditions, it has activity in theory. This protein has glycosylation modification, we recommended the eukaryotic expression system, the possibility of activity will be higher.
The detail of CSB-EP011660DYB is as follow:
Recombinant Crotalus adamanteus L-amino-acid oxidase
CSB-EP011660DYB >> E.coli
Express region:19-516aa；Mature full length protein.
Tag information:N-terminal 10xHis-tagged and C-terminal Myc-tagged；
Sequence:

AHDRNPLEECFRETDYEEFLEIAKNGLTATSNPKRVVIVGAGMAGLSAAYVLAGAGHQVTVLEASERVGGRVRTYRKKDWYANLGPMRLPTKHRIVREYIKKFDLKLNEFSQENENAWYFIKNIRKRVREVKNNPGLLEYPVKPSEEGKSAAQLYVESLRKVVEELRSTNCKYILDKYDTYSTKEYLLKEGNLSPGAVDMIGDLLNEDSGYYVSFIESLKHDDIFGYEKRFDEIVGGMDQLPTSMYEAIKEKVQVHFNARVIEIQQNDREATVTYQTSANEMSSVTADYVIVCTTSRAARRIKFEPPLPPKKAHALRSVHYRSGTKIFLTCTKKFWEDDGIHGGKSTTDLPSRFIYYPNHNFTSGVGVIIAYGIGDDANFFQALDFKDCADIVINDLSLIHELPKEDIQTFCHPSMIQRWSLDKYAMGGITTFTPYQFQHFSEALTAPFKRIYFAGEYTAQFHGWIDSTIKSGLTAARDVNRASENPSGIHLSNDNEF


Expcept the activity, do you have other request? For example:Aseptic Processing and Endotoxin Removal? We could provide these service for free.
PS:we also provide the protein cutomised service. Pls refer to : http://www.cusabio.com/protein_service/

靶点详情

  • 功能：
    Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids, thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Is active on L-Arg, L-Phe, L-Met, and L-Leu and is weakly active on L-Val. Exhibits diverse biological activities, such as hemorrhage, hemolysis, edema, apoptosis of vascular endothelial cells or tumor cell lines, antibacterial and antiparasitic activities, as well as regulation of platelet aggregation. Its effect on platelets is controversial, since it either induces aggregation or inhibits agonist-induced aggregation. These different effects are probably due to different experimental conditions.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Flavin monoamine oxidase family, FIG1 subfamily
  • 组织特异性：
    Expressed by the venom gland.