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Recombinant Carica papaya Papain

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  • 货号：
    CSB-EP365471DQH
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    Papain; EC 3.4.22.2; Papaya proteinase I; PPI; allergen Car p 1
  • 种属：
    Carica papaya (Papaya)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    27.4kDa
  • 表达区域：
    134-345aa
  • 氨基酸序列
    IPEYVDWRQKGAVTPVKNQGSCGSCWAFSAVVTIEGIIKIRTGNLNEYSEQELLDCDRRSYGCNGGYPWSALQLVAQYGIHYRNTYPYEGVQRYCRSREKGPYAAKTDGVRQVQPYNEGALLYSIANQPVSVVLEAAGKDFQLYRGGIFVGPCGNKVDHAVAAVGYGPNYILIKNSWGTGWGENGYIRIKRGTGNSYGVCGLYTSSFYPVKN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The process of expressing this Carica papaya Papain recombinant protein in E.coli expression system requires the following steps: use of competent E. coli cells to take up DNA sequence of interest, the exogenous plasmid is transferred into the cell, and the transcription and translation are directly replicated in the cell, selection of transformed E. coli using a selection marker, expansion of selected E. coli to a higher scale in appropriate culture media, such as classic LB options Growth Systems and lastly isolation and purification.And we finally get a Papain protein with N-terminal 6xHis tag and it is determined by SDS-PAGE with a purity of 90%+.

    Papain (also called papaya proteinase I) is a cysteine protease enzyme present in papaya and mountain papaya. According to some research, Gsta1 may have the following features.
    Compared to other carbohydrate polymers, papain entrapped in starch gels showed better stability and activity retention when added to household detergent powders. The inactivation of papain by S-nitrosothiols results from a direct attack on the highly reactive sulfate at the active site of the enzyme. Papain was probably synthesized as a 133-aa fragment, suggesting that the enzyme was synthesized as an inactive zymogen.

  • Datasheet & COA：
    Please contact us to get it.

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