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  4. Recombinant Caenorhabditis elegans Fem-3 mRNA-binding factor 1 (fbf-1), partial

Recombinant Caenorhabditis elegans Fem-3 mRNA-binding factor 1 (fbf-1), partial

  • 中文名称：
    秀丽隐杆线虫fbf-1重组蛋白
  • 货号：
    CSB-YP885648CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    秀丽隐杆线虫fbf-1重组蛋白
  • 货号：
    CSB-EP885648CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    秀丽隐杆线虫fbf-1重组蛋白
  • 货号：
    CSB-EP885648CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    秀丽隐杆线虫fbf-1重组蛋白
  • 货号：
    CSB-BP885648CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    秀丽隐杆线虫fbf-1重组蛋白
  • 货号：
    CSB-MP885648CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    fbf-1
  • Uniprot No.：
  • 别名：
    fbf-1; H12I13.4Fem-3 mRNA-binding factor 1
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the control of stem cells and sex determination in the C.elegans hermaphrodite germline. May also play a role in the hermaphrodite germline proliferation and oogenesis. Binds specifically to the regulatory region of fem-3 3'-UTR and mediates the sperm/oocyte switch. Negatively regulates gld-3 expression, possibly by directly binding to two sites within the 3'-UTR of gld-3 isoform b. In association with the cye-1/cdk-2 complex, negatively regulates gld-1 expression in the distal germline cells of the mitotic zone. By binding to the 3'-UTR, represses phosphatase lip-1 expression in the distal part of the germline mitotic zone. Suppresses germline tumor formation by preventing the dedifferentiation of secondary spermatocytes.
  • 基因功能参考文献：
    1. It was concluded that FBF1 is a key functional transition fibre component that actively facilitates the ciliary entry of assembled IFT machinery. PMID: 24231678
    2. Data show that CPB-1 (cytoplasmic polyadenylation element binding protein homolog-1) alters the affinity of FBF (fem-3 mRNA binding factor) for specific RNA sequences. PMID: 23159558
    3. Data indicate that a 10-amino-acid region in germline development defective-3 (GLD-3) is required for fem-3 binding factor (FBF) binding, while a 7-amino-acid loop in FBF between PUF repeats 7 and 8 is necessary for GLD-3 binding. PMID: 23159559
    4. Here, the s report that FBF/Pumilio, a conserved RNA-binding protein, promotes self-renewal of germline stem cells by repressing CKI-2(Cip/Kip), a Cyclin E/Cdk2 inhibitor. PMID: 21822213
    5. The Puf RNA-binding proteins FBF-1 and FBF-2 inhibit the expression of synaptonemal complex proteins in germline stem cells. PMID: 20431119
    6. fbf-1 regulates the size of the mitotic region in helminth germ cells. PMID: 15525531
    7. FBF may coordinately repress sperm-specifying mRNAs to direct oogenesis and it represses the fog-1 mRNA to maintain FOG-1 at a low level appropriate for proliferation PMID: 16000383
    8. all fbf-1 puf-8 double mutants fail in the hermaphrodite sperm/oocyte switch PMID: 16037210
    9. A structural distortion in the central region of FBF imposes the requirement for an additional nucleotide to effect messenger RNA binding, thus defining its specificity. PMID: 16244662
    10. Fbf-1 redundantly inhibits primary vulval cell fate specification in two distinct pathways acting in the soma and in the germline PMID: 16908630
    11. Results show that the RNA-binding PUF protein FBF-1 is required in the adult olfactory sensory neurons of C. elegans for adaptation. PMID: 19146813

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  • 亚细胞定位：
    Cytoplasm.
  • 组织特异性：
    Expressed specifically in the germline (at protein level).
  • 数据库链接：

    KEGG: cel:CELE_H12I13.4

    STRING: 6239.H12I13.4

    UniGene: Cel.23295