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  4. Recombinant Bovine Rab5 GDP/GTP exchange factor (RABGEF1)

Recombinant Bovine Rab5 GDP/GTP exchange factor (RABGEF1)

  • 货号：
    CSB-YP019233BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019233BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019233BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019233BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019233BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RABGEF1
  • Uniprot No.：
  • 别名：
    RABGEF1; RABEX5; Rab5 GDP/GTP exchange factor; Rabex-5
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-492
  • 氨基酸序列
    MSLKSERRGI HVDQSELLCK KGCGYYGNPA WQGFCSKCWR EEYHKARQKQ IQEDWELAER LQREEEEAFA SSQSSQGAQS LTFSKFEEKK TNEKTRKVTT VKKFFSASSR VGAKKAEIQE AKAPSPSINR QTSIETDRVS KEFIEFLKTF HKTGQEIYKQ TKLFLEAMHY KRDLSIEEQS ECTQDFYQNV AERMQTRGKV PPERVEKIMD QIEKYIMTRL YKYVFCPETT DDEKKDLAIQ KRIRALHWVT PQMLCVPVNE EIPEVSDMVV KAITDIIEMD SKRVPRDKLA CITKCSKHIF NAIKITKNEP ASADDFLPTL IYIVLKGNPP RLQSNIQYIT RFCNPSRLMT GEDGYYFTNL CCAVAFIEKL DAQSLNLSQE DFDRYMSGQT SPRKQESENW SPDACLGVKQ MYKNLDLLSQ LNERQERIMN EAKKLEKDLI DWTDGIAKEV QDIVEKYPLE IRPPNQSVAA IDSENVENDK LPPPLQPQVY AG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Rab effector protein acting as linker between gamma-adaptin and RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase.
  • 基因功能参考文献：
    1. these data suggest differential physiological roles of the two ubiquitin binding domains in Rabex-5. PMID: 22705550
    2. Receptor recycling is mediated by AP-1/clathrin-coated vesicles and regulated by rab4 and rabaptin-5/rabex-5. PMID: 15331762
    3. Rabex-5 is a ubiquitin ligase that binds ubiquitin and undergoes ubiquitination; the N-terminal region of Rabex-5 (residues 1-76) is important for ubiquitin binding and ubiquitination. PMID: 16407276
    4. The structure of the N-terminal portion of Rabex-5 bound to ubiquitin at 2.5-A resolution shows that Rabex-5-ubiquitin interactions occur at two sites PMID: 16462746
    5. Rabex-5 can target to early endosomes via the EET domain and activate Rab5 in a Rabaptin-5-independent manner in vivo. PMID: 17699593
    6. Data show that ubiquitin binding is essential for the recruitment of Rabex-5 from the cytosol to endosomes, independently of its guanine nucleotide exchange factor activity and of Rab5. PMID: 18772883

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  • 亚细胞定位：
    Cytoplasm. Early endosome. Recycling endosome.
  • 组织特异性：
    Detected in brain.
  • 数据库链接：

    KEGG: bta:282335

    STRING: 9913.ENSBTAP00000005011

    UniGene: Bt.317