Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Bovine Protein S100-A8 (S100A8)

Recombinant Bovine Protein S100-A8 (S100A8)

  • 货号：
    CSB-YP020641BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP020641BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP020641BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP020641BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP020641BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    S100A8Protein S100-A8; BEE11; Calgranulin-A; Neutrophil cytosolic 7 kDa protein; P7; S100 calcium-binding protein A8
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-89
  • 氨基酸序列
    MLTDLECAIN SLIDVYHKYS LKKGNYHAVY RDDLKQLLET ECPKFMKKKD ADTWFKELDI NQDGGINFEE FLVLVIKVGL EAHEEIHKE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：
    牛蛋白S100-A8（S100A8）是一种小分子的钙调蛋白，参与了多种细胞功能的调节和信号传导。如果想要重组这种蛋白，可以按照以下步骤进行：
    克隆S100A8基因：可以使用PCR技术从牛的基因组DNA中扩增S100A8基因的开放阅读框（ORF）序列。此外，还需要添加一些启动子和标签序列，以便在表达过程中调节基因表达，并且可以方便地检测到蛋白质。
    构建表达载体：将S100A8基因插入表达载体中，可以选择一些经过验证的表达载体，如pET系列载体。这些载体中包含了一些促进蛋白表达的元件，如启动子、增强子、终止子等。
    选择表达宿主菌：可以选择一些常用的表达宿主菌，如大肠杆菌（E. coli），并在菌株中转化含有S100A8基因的表达载体。这些宿主菌中还需要添加一些辅助蛋白质，如分泌蛋白、分子伴侣等，以帮助蛋白质的正确折叠和纯化。
    蛋白表达和纯化：在表达宿主菌中诱导S100A8蛋白的表达，并通过细胞破碎和离心等方法提取蛋白质。然后使用亲和层析、离子交换层析、凝胶过滤层析等方法对蛋白进行纯化。
    蛋白质的鉴定和活性检测：使用SDS-PAGE、Western blot等方法对纯化后的S100A8蛋白进行鉴定和定量。此外，还可以使用细胞实验等方法对S100A8蛋白的生物活性进行检测，如检测其对细胞的增殖、分化、迁移等的影响。
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. The iNOS-S100A8/A9 transnitrosylase complex is proposed to direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as GAPDH, ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity.
  • 基因功能参考文献：
    1. The findings indicated that plasma concentrations of S100A7 and S100A8 did not change significantly during pregnancy in cows. PMID: 29604144
    2. study of nonsynonymous DNA variation in the functional domain of the S100A7, -A8, -A9 genes in taurine cattle and yak PMID: 29774569
    3. Data suggest that up-regulation of S100A8 and S100A9 is a key component of early endometrial response to uterine involution in the post-partum period and to prevent chronic endometritis/uterine inflammation; up-regulation can be influenced by diet. PMID: 23533291
  • 亚细胞定位：
    Secreted. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein.
  • 蛋白家族：
    S-100 family
  • 组织特异性：
    Found essentially in phagocytic cells.
  • 数据库链接：

    KEGG: bta:616818

    STRING: 9913.ENSBTAP00000016774

    UniGene: Bt.9360