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  4. Recombinant Bovine Neutrophil cytosol factor 1 (NCF1)

Recombinant Bovine Neutrophil cytosol factor 1 (NCF1)

  • 货号：
    CSB-YP015525BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015525BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015525BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015525BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015525BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    NCF1; Neutrophil cytosol factor 1; NCF-1; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; p47-phox
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-392
  • 氨基酸序列
    MGDHFIRHIA LLGFEKRFVP SQHYVYMFLV KWQDLSEKVV YRRFTEIYEF HKILKEMFPI EAGDINPENR IIPHLPAPRW YDGQRVAESR QGTLTEYCST LMSLPVKISR CPHLLNFFKV RPDDLKLPTD SQVKKPETYL MPKDGKNNAA DITGPIILQT YRAIADYEKG SSSQMALATG DVVDVVEKNE SGWWFCQMKT KRGWVPASYL EPLDSPDEAE DPEPNYAGEP YITIKAYTAV LEDEISLEEG EAIEVIHKLL DGWWVIRKED VTGYFPSMYL QKAGQDVAQA KSQIKSRGAP PRRSSIRNAH SIHQRSRKRL SQDTYRRNSV RFMQQRRHQR PEPQRSRSAL REQQQPKTER PKPQPAVPPR PSADLILHRC SESTKRKLAS AV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
  • 基因功能参考文献：
    1. Differential Roles of Protein Complexes NOX1-NOXO1 and NOX2-p47phox in Mediating Endothelial Redox Responses to Oscillatory and Unidirectional Laminar Shear Stress. PMID: 26826128
    2. two novel splice variants designed as NCF1-TV1 (retained intron 6) and NCF1-TV2 (retained part of intron 8)encoding two putative truncated proteins (239AA and 283AA). Two splice variants were up-regulated in the mastitis-infected cows' mammary tissues, blood and neutrophils compared with these of healthy cows. PMID: 25946326
    3. A p47(phox) and Src kinase activation of peroxide production by Nox2 appears to be an important contributor to vascular contractile mechanisms mediated through activation of protein kinase C. PMID: 19168729
  • 亚细胞定位：
    Cytoplasm, cytosol. Membrane; Peripheral membrane protein; Cytoplasmic side.
  • 数据库链接：

    KEGG: bta:281345

    STRING: 9913.ENSBTAP00000004279

    UniGene: Bt.142