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  4. Recombinant Bovine Leptin (LEP)

Recombinant Bovine Leptin (LEP)

  • 货号：
    CSB-YP012870BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP012870BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP012870BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP012870BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP012870BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    LEP; OB; OBS; Leptin; Obesity factor
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-167
  • 氨基酸序列
    VPIRKVQDD TKTLIKTIVT RINDISHTQS VSSKQRVTGL DFIPGLHPLL SLSKMDQTLA IYQQILTSLP SRNVVQISND LENLRDLLHL LAASKSCPLP QVRALESLES LGVVLEASLY STEVVALSRL QGSLQDMLRQ LDLSPGC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways. In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity. In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides. In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption. Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways. May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression. Pro-angiogenic, has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). In innate immunity, modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells. Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 wich promotes the production of IL6, IL8 and Prostaglandin E2, through a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38. In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses. Increases CD4(+)CD25(-) T-cell proliferation and reduces autophagy during TCR (T-cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation.
  • 基因功能参考文献：
    1. Consistent with this, leptin enhanced GnRH-induced secretion of LH measured by ELISA. We suggest that leptin enhances membrane expression of voltage-gated Na(+) and Ca(2+) channels, which results in a modulation of the action potential properties and an increase in hormone release from gonadotropes. PMID: 28705737
    2. The effects of a single nucleotide polymorphism of leptin on weight gain and body composition are reported. PMID: 28177355
    3. A leptin SNP (LEPg.978) was significantly associated with a weight at one year. PMID: 27706683
    4. The results of this study suggest that leptin physiology could be a candidate for mechanisms that contribute to feed intake and feed efficiency variation in beef cattle. PMID: 26851619
    5. Leptin concentrations could be a useful physiological marker for growth and feed efficiency of finishing beef cattle. PMID: 26440340
    6. Hormonal gene expression involved in residual feed intake in dairy cows may be related to the molecular regulation of the leptin-NPY and insulin signaling pathways. PMID: 26231801
    7. Leptin R25C genotype impacted most traits associated with fatness. PMID: 24753381
    8. The absence of A80V polymorphism (C --> T at position 95691973 bp of leptin gene) has been established in the genotypes of Ayrshire cattle as compared to Holstein cattle. PMID: 25966594
    9. Data indicate that increased body weight gain during juvenile development accelerates sexual maturation in heifers, coincident with reciprocal changes in circulating concentrations of leptin and hypothalamic neuropeptide Y (NPY) release. PMID: 25326602
    10. It is an important regulator of metabolic efficiency, energy expenditure, food intake and body fat mass. PMID: 23910951
    11. SNP LEP significantly affected milk, protein and fat yield (P<0.05), and age at first calving (P<0.01) in analyzed population of cows. SNP LEPR/T945M affected significantly calving interval (P<0.01) only PMID: 24432332
    12. Results indicated that leptin R25C genotype impacted most traits associated with fatness whereas feeding zilpaterol for 21 d affected weight gain positively but impacted other variables negatively. PMID: 23942708
    13. There is a strong association between putative favorable allelic variants (SNP) of neuropeptide Y, leptin, and IGF-1 genes, and residual feed intake, when animals were grazing on a high-quality, high-availability pasture. PMID: 23881687
    14. The LEP, IGF2 and CCL2 genes showed allelic expression imbalance in liver, kidney and pituitary glands of Polish Holstein-Friesian bulls. PMID: 23184004
    15. The aim of the study was to determine by immunochemistry the expression of leptin, orexin A and orphanin FQ in the major salivary glands (parotid, submandibular and sublingual) of rat, sheep and cow. PMID: 23264212
    16. The use of leptin, leptin receptor, and DGAT1 polymorphisms as markers within genetic selection programs to improve and adjust several compositional parameters. PMID: 22127264
    17. The leptin and receptor gene markers would be useful for marker-assisted selection. PMID: 22930433
    18. A significant positive relationship exists between the T allele frequency in the bovine leptin C/T SNP and animal backfat, with TT, CT and CC animals having estimates of 6.79 +/- 0.02, 6.49 +/- 0.01 and 6.28 +/- 0.01 mm, respectively. PMID: 22497203
    19. The impact of different alleles of DGAT1 and leptin on milk production in Giorlando cattle is reported. PMID: 21396671
    20. The effects of dietary energy on the metabolism of ghrelin, leptin and growth hormone secretagogue receptor in blood and tissues of steers are reported. PMID: 21724941
    21. This study provides evidence that leptin gene polymorphisms play a role in the variability of hematological variables during the peripartum period, and might be used as genetic markers to improve dairy cow immunological conditions in productive periods. PMID: 21890501
    22. This study reports changes in plasma leptin concentration parallel to changes in the gene expression of lipogenic- and lipolytic-related genes in adipose tissue of dairy cows around parturition. PMID: 21198961
    23. Gene expression of leptin was affected by parental origin in cattle. PMID: 21899958
    24. Cows homozygous for the T allele were less likely to be cyclic early postpartum, but leptin genotype was not associated with other reproductive responses when cows were subjected to a controlled breeding program. PMID: 21917388
    25. Mixed model analyses revealed that leptin SNP were associated with early skeletal growth (height, A1457G; length, A59V), fertility (UASMS1, UASMS2, A1457G, A59V) and milk production (A59V). PMID: 21700051
    26. Fundamental role of SCD1 genes in transforming saturated fatty acids into monounsaturated fatty acids is mediated by the leptin gene. PMID: 21145173
    27. polymorphisms in the leptin gene were associated with 2-fold differences in perinatal mortality in dairy heifers PMID: 20059932
    28. the possible involvement of locally produced leptin/Ob-R system in the bovine ovary, suggesting roles in the function and/or development of the CL and growth of small follicles in an autocrine/paracrine fashion. PMID: 19908249
    29. Leptin tended to suppress serum growth hormone in cattle PMID: 19632078
    30. Genomic structure and promoter analysis of the bovine leptin gene PMID: 12049196
    31. higher leptin concentrations associated with shorter intervals to first observed estrus might indicate a relationship between leptin and expression of estrus PMID: 12703616
    32. insulin increases plasma leptin in late pregnancy by stimulating adipose tissue synthesis but has only marginal effects in early lactation, when cows are in negative energy balance PMID: 12881203
    33. findings indicate that acetate and butyrate enhance leptin expression in bovine, but not in rat anterior pituitary cells while butyrate suppresses leptin receptor expression in both rat and bovine pituitaries PMID: 12928006
    34. exogenous leptin can prevent fasting-mediated reductions in the frequency of LH pulses and modify GnRH-mediated release of LH in intact, prepubertal heifers. PMID: 14522828
    35. cattle homozygous for the leptin T allele produced more milk and had higher somatic cell count PMID: 14594235
    36. plasma leptin is regulated by nutrition in early postnatal life, but that a sudden increase in plasma leptin is not required for the onset of puberty in dairy cattle PMID: 14594240
    37. association of four leptin gene polymorphisms in dairy cows (R4C, A59V, RFLP1, and BM1500) with circulating leptin concentrations during the periparturient period PMID: 14629116
    38. leptin is unable to accelerate the pulse generator of LH and GnRH in heifers at any developmental stage. PMID: 15128593
    39. Dysregulation of PL and leptin in nuclear transfer placentomes could affect cell migration and invasion and subsequently placental metabolism and transfer of nutrients to fetus, leading to increased placental and fetal macrosomia. PMID: 15306554
    40. leptin plays an important role in mammary gland lactogenesis and the expression of leptin requires the presence of prolactin PMID: 15375055
    41. study reports associations between SNP in the 5' untranslated promoter region of the leptin gene with serum leptin concentration, growth, BW, feed intake, feeding behavior, and carcass merit PMID: 15583038
    42. Important associations between SNP within the leptin gene with lean yield, fatness (fat yield and subcutaneous fat) and tenderness were detected. PMID: 16100055
    43. study shows that there is a relationship between the concentration of leptin in follicular fluid and atresia in small follicles PMID: 16363147
    44. analysis of CpG islands of bovine Leptin and POU5F1 genes in cloned bovine fetuses PMID: 16474211
    45. observations reveal an important linkage between pulsatile luteinizing hormone(LH) secretion and blood leptin concentrations during the early postpartum period in dairy cows PMID: 16840618
    46. Characterization of single nucleotide polymorphisms in LEP in relation to production traits. PMID: 16879354
    47. Sheep, goat and cattle R-banded chromosome preps, obtained from synchronized cell cultures, were used to FISH-map leptin and SLC26A2 genes on single chromosome bands. LEP maps on OAR4q32 and CHI4q32. Improved cytogenetic maps of BTA4/OAR4/CHI4. PMID: 16974077
    48. results suggest that plasma leptin levels in cows are inversely controlled at the transcription level by volatile fatty acids(VFA) and long-chain fatty acids (LCFA) PMID: 17011156
    49. Modulatory effect of leptin on luteal progesterone production in vitro. PMID: 17154301
    50. In conclusion, this study revealed significant differences in body weight and backfat growth parameters across leptin genotypes for a sample of commercially fed beef cattle. PMID: 17431048

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Leptin family
  • 数据库链接：

    KEGG: bta:280836

    STRING: 9913.ENSBTAP00000019853

    UniGene: Bt.1939