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  4. Recombinant Bovine Histone H1oo (H1FOO)

Recombinant Bovine Histone H1oo (H1FOO)

  • 货号：
    CSB-YP662993BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP662993BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP662993BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP662993BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP662993BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    H1FOO
  • Uniprot No.：
  • 别名：
    H1FOO; H1OO; Histone H1oo; Oocyte-specific histone H1; Oocyte-specific linker histone H1
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-343
  • 氨基酸序列
    MAPGSIASSD TSSSTSSSST SSASSASAEG SSRPLGSEKP GLARGAVRAP RRHPPVLRMV LEALQAGERR RGTSVAAIKV YILQKYPTVD ALRLNHLLKQ ALATGLHRGL LIRPVNSKAK GATGSFKLVP KDKRKIPPRK TAPRMPGQAE GKDPKKPSES KKDPANTVEV KKGSRKPREE RAAPSKPGAA KKAPKKGTQT KDPEPRLGEA KKSSRRPDKA AQAPPSAGGP GGKSKVKERG SRQADTKAHR KTQPGSQSSK STVTKGENGA PLAKKKMGGK VPKEAAGEGP KAKAPVPPKG AGSKKEPGPL AGKAEASKGP RKPGIPTKSS VSKAASKKAE AEG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.
  • 基因功能参考文献：
    1. H1foo is essential for bovine oocyte meiotic maturation and its overexpression stimulates the process. PMID: 24618348
    2. Results describe the characterization of linker histone H1FOO during bovine in vitro embryo development. PMID: 16470586
  • 亚细胞定位：
    Cytoplasm. Nucleus. Chromosome. Note=In the 1-, 2- and 4-cell embryo, it is uniformly spread within the cytoplasm, while it is concentrated onto the chromatin in the nucleus. In the 8- to 16-cell embryo, its presence diminishes in the cytoplasm, although it is still strongly expressed in the nucleus.
  • 蛋白家族：
    Histone H1/H5 family
  • 组织特异性：
    Oocyte (at protein level).
  • 数据库链接：

    KEGG: bta:527400

    STRING: 9913.ENSBTAP00000002369

    UniGene: Bt.36838