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  4. Recombinant Bovine Glucosylceramidase (GBA)

Recombinant Bovine Glucosylceramidase (GBA)

  • 货号：
    CSB-YP640659BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP640659BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP640659BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP640659BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP640659BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GBA
  • Uniprot No.：
  • 别名：
    GBALysosomal acid glucosylceramidase; EC 3.2.1.45; Acid beta-glucosidase; Beta-glucocerebrosidase; Cholesterol glucosyltransferase; SGTase; EC 2.4.1.-; Cholesteryl-beta-glucosidase; EC 3.2.1.104; D-glucosyl-N-acylsphingosine glucohydrolase
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    40-536
  • 氨基酸序列
    A RPCSPKSFGY SSVVCVCNGT YCDSLDPLTL PDPGTFSRFE STRSGRRMEL SLGTIQANRT GTGLLLTLQP DQKFQKVKGF GGAMTDAAAL NILALSPAAR NLLLKSYFSE EGIEYNIIRV PMASCDFSIR TYTYDDSPDD FQLLNFSLPE EDVKLKIPLI HQALELANRS VSLFASPWTS PTWLKTNGAV NGKGTLKGQA GDLYHKTWAR YFVKFLDAYA EHKLRFWAVT AENEPTAGLL TGYPFQCLGF TPEHQRDFIA RDLGPILANS THRDVRLLML DDQRLLLPRW AQVVLADPEA AKYVHGIAVH WYLDFLAPAK ATLGETHRLF PNTMLFASEA CVGSKFWEQS VRLGSWDRGM RYSHSIITNL LYHVVGWTDW NLALNPEGGP NWVRNFVDSP IIVDIAKDTF YKQPMFYHLG HFSKFIPEGS QRVGLVASKK SDLDTVALLR PDGSAVAVVL NRSSKDVPLT IKDPAVGFME TVSPGYSIHT YLWRRQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose. Thereby, plays a central role in the degradation of complex lipids and the turnover of cellular membranes. Through the production of ceramides, participates in the PKC-activated salvage pathway of ceramide formation. Also plays a role in cholesterol metabolism. May either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol. The short chain saturated C8:0-GlcCer and the mono-unsaturated C18:0-GlcCer being the most effective glucose donors for that transglucosylation reaction. Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide. Finally, may also hydrolyze cholesteryl-beta-D-glucoside to produce D-glucose and cholesterol.
  • 亚细胞定位：
    Lysosome membrane; Peripheral membrane protein; Lumenal side.
  • 蛋白家族：
    Glycosyl hydrolase 30 family
  • 数据库链接：

    KEGG: bta:537087

    STRING: 9913.ENSBTAP00000019765

    UniGene: Bt.5413