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  4. Recombinant Bovine Epididymal secretory protein E1 (NPC2)

Recombinant Bovine Epididymal secretory protein E1 (NPC2)

  • 货号：
    CSB-YP015976BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015976BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015976BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015976BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015976BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    NPC2; NPC intracellular cholesterol transporter 2; EPV20; Epididymal secretory protein E1; Niemann Pick type C2 protein homolog
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    20-149
  • 氨基酸序列
    E PVKFKDCGSW VGVIKEVNVS PCPTQPCKLH RGQSYSVNVT FTSNTQSQSS KAVVHGIVMG IPVPFPIPES DGCKSGIRCP IEKDKTYNYV NKLPVKNEYP SIKVVVEWEL TDDKNQRFFC WQIPIEVEA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. May bind and mobilize cholesterol that is associated with membranes. NPC2 binds cholesterol with a 1:1 stoichiometry. Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol. The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport.
  • 基因功能参考文献：
    1. Data suggest ASM (acid sphingomyelinase) activity is regulated by membrane lipids and facilitates cholesterol transfer by NPC2 (Niemann Pick protein type C2); hydrolysis of sphingomyelin by ASM may be crucial for endosomal lipid degradation/sorting. PMID: 25339683
    2. Data show that the effects of the lipids on cholesterol transfer mediated by NPC2 were similar to their effect on membrane fusion induced by NPC2 and saposin-C. PMID: 20179319
    3. bNPC2 has a loosely packed region penetrating from the surface into the hydrophobic core that forms adjacent small cavities and represents an incipient cholesterol-binding site PMID: 12591954
    4. analysis of sterol binding by NPC2, a lysosomal protein deficient in Niemann-Pick type C2 disease PMID: 17573352
    5. The seminal plasma protein, Niemann-Pick C2 protein, is involved in cholesterol and GM1 depletion within detergent-resistant membrane, then leading to membrane redistribution of P25b that occurs in a very rapid and capacitation-independent manner. PMID: 18235103
    6. NPC2 plays an important role in endo/lysosomal cholesterol trafficking by markedly accelerating the rates of cholesterol transport. Rates of sterol transfer from and between membranes were increased by as much as 2 orders of magnitude by NPC2. PMID: 18823126

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  • 亚细胞定位：
    Secreted. Endoplasmic reticulum. Lysosome.
  • 蛋白家族：
    NPC2 family
  • 组织特异性：
    Expressed in kidney, spleen, liver and mammary gland, but not in testis.
  • 数据库链接：

    KEGG: bta:280815

    STRING: 9913.ENSBTAP00000029271

    UniGene: Bt.23268