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  4. Recombinant Bovine Cytochrome b561 (CYB561), partial

Recombinant Bovine Cytochrome b561 (CYB561), partial

  • 货号：
    CSB-YP006306BO1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP006306BO1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP006306BO1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP006306BO1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP006306BO1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CYB561
  • Uniprot No.：
  • 别名：
    CYB561; Transmembrane ascorbate-dependent reductase CYB561; Chromomembrin B; Cytochrome b-561; Cytochrome b561
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transmembrane reductase that uses ascorbate as an electron donor in the cytoplasm and transfers electrons across membranes to reduce monodehydro-L-ascorbate radical in the lumen of secretory vesicles. It is therefore involved the regeneration and homeostasis within secretory vesicles of ascorbate which in turn provides reducing equivalents needed to support the activity of intravesicular enzymes (Probable).
  • 基因功能参考文献：
    1. the functional and structural roles of the EM3 residues and several adjacent residues (residues 70-85) in the bovine cytochrome PMID: 21401125
    2. Cyb561 from chromaffin cell membranes has high and low potential hemes as demonstrated by electron paramagnetic resonance and circular dichroism. PMID: 12438163
    3. The pH-induced alteration and the destruction of heme in cytochrome b561 under oxidative conditions may play a significant role in the amplification of oxidative stress in catecholaminergic neurons. PMID: 12653566
    4. amino-terminal peptide showed that the initial Met residue was acetylated but there was no other posttranslational modification in the amino-terminal region PMID: 12768340
    5. Purified cytochrome b561 reconstituted into an artificial phospholipid bilayer is capable of transferring electron equivalents across the membrane to support monooxygenase activity of soluble extravesicular dopamine beta-hydroxylase. PMID: 14769875
    6. Raman data indicate that the two heme b centers of CYB561 adopt a six-coordinated low-spin state, in both reduced and oxidized forms. PMID: 14999009
    7. importance of the two well-conserved Cys residues near the intravesicular heme center PMID: 16428304
    8. a detailed characterization of cyt b561 using electron paramagnetic resonance (EPR) and optical spectroscopy on the wild-type and mutant forms of the cytochrome expressed in insect cells PMID: 17602662
    9. These results indicate that His92 can be considered part of the b(H) heme center, and His110 part of the b(L) heme center, in adrenal cyt b(561). PMID: 18501187

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  • 亚细胞定位：
    Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Multi-pass membrane protein.
  • 组织特异性：
    Expressed in the adrenal medulla and all brain regions, but not in visceral organs.
  • 数据库链接：

    KEGG: bta:317663

    STRING: 9913.ENSBTAP00000027732

    UniGene: Bt.4360