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  4. Recombinant Bovine Chromogranin-A (CHGA)

Recombinant Bovine Chromogranin-A (CHGA)

  • 货号：
    CSB-YP005344BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005344BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005344BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005344BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005344BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CHGAChromogranin-A; CgA; Pituitary secretory protein I; SP-I) [Cleaved into: Vasostatin-1; Chromofungin; Chromostatin; Chromacin; Pancreastatin; WE-14; Catestatin; GE-25; Serpinin-RRG; Serpinin; p-Glu serpinin precursor]
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    19-449
  • 氨基酸序列
    LP VNSPMNKGDT EVMKCIVEVI SDTLSKPSPM PVSKECFETL RGDERILSIL RHQNLLKELQ DLALQGAKER THQQKKHSSY EDELSEVLEK PNDQAEPKEV TEEVSSKDAA EKRDDFKEVE KSDEDSDGDR PQASPGLGPG PKVEEDNQAP GEEEEAPSNA HPLASLPSPK YPGPQAKEDS EGPSQGPASR EKGLSAEQGR QTEREEEEEK WEEAEAREKA VPEEESPPTA AFKPPPSLGN KETQRAAPGW PEDGAGKMGA EEAKPPEGKG EWAHSRQEEE EMARAPQVLF RGGKSGEPEQ EEQLSKEWED AKRWSKMDQL AKELTAEKRL EGEEEEEEDP DRSMRLSFRA RGYGFRGPGL QLRRGWRPNS REDSVEAGLP LQVRGYPEEK KEEEGSANRR PEDQELESLS AIEAELEKVA HQLEELRRG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Strongly inhibits glucose induced insulin release from the pancreas.; Completely inhibits catecholamine release from chromaffin cells.; Has antibacterial activity against M.luteus. Not active against E.coli.; Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Displays antibacterial activity against Gram-positive bacteria M.luteus and B.megaterium, and Gram-negative bacteria E.coli, and antifungal activity against a variety of filamentous fungi including A.fumigatus, N.hematococca, F.culmorum, F.oxyporum, T. mentagrophytes and several forms of Candida: C.albicans, C.tropicalis, C.glabrata and C.neoform. Can induce mast cell migration, degranulation and production of cytokines and chemokines.; Has antibacterial activity against Gram-positive bacteria M.luteus, B.megaterium. Not active against Gram-positive bacteria B.cereus, B.subtilis, S.pyogenes, M.fortuitum, S.aureus and L.monocytogenes and against Gram-negative bacteria E.coli, E.cloacae, S.typhimurium, K.pneumoniae and P.aeruginosa. Possesses antifungal activity against N.crassa, A.fumigatus, A.brassicicola, N.hematococca, F.culmorum and F.oxyporum and against the yeast S.cerevisiae and C.albicans. Inactive against A.benhamiae.; Has antifungal activity against N.crassa, A.fumigatus, A.brassicicola, N.hematococca, F.culmorum, F.oxyporum, A.benhamiae, C.neoformans, as well as against yeasts C.albicans, and C.tropicalis. Seems to be inactive against C.glabrata. Interacts with the fungal cell wall, crosses the plasma membrane and accumulates in fungal cells where it inhibits calcineurin activity.; Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
  • 基因功能参考文献：
    1. Vasoconstriction-Inhibiting Factor (VIF), a degradation product of chromogranin A, is a vasoregulatory peptide that modulates the vasoconstrictive effects of angiotensin II by acting on the angiotensin II type 2 receptor. PMID: 25810338
    2. chromogranin A has a role in the IP(3)-mediated Ca(2+) release mechanism of secretory granules PMID: 12438140
    3. chromogranin A has a specific site in the N-terminal domain that can bind membrane lipids from different species PMID: 12438150
    4. role of coupling with the inositol 1,4,5-trisphosphate receptor/Ca2+ channel (InsP3R)in the Ca2+-dependent ciliary movement PMID: 12725433
    5. involvement of CGA with other components of the senile plaque PMID: 15342341
    6. significant species differences in vasoactivity of the N-terminal domain of ChgA PMID: 15544843
    7. determination of the subcellular distribution of chromogranins A and B in chromaffin cells; results suggest that chromogranins are at the center of intracellular Ca(2+) homeostasis in secretory cells PMID: 16140299
    8. Catestatin (chromogranin A344-364) is a novel cardiosuppressive agent: inhibition of isoproterenol and endothelin signaling in the frog heart. PMID: 18469147
    9. Cateslytin adopts a dominant beta-sheet character upon interaction with negatively charged membranes, whereas it is essentially unstructured in water as are most short linear antimicrobial peptides. PMID: 18500827

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  • 亚细胞定位：
    [Serpinin]: Secreted. Cytoplasmic vesicle, secretory vesicle.; Cytoplasmic vesicle, secretory vesicle. Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle. Secreted.
  • 蛋白家族：
    Chromogranin/secretogranin protein family
  • 组织特异性：
    Highest concentration of GE-25 found in adrenal medulla with lower levels present in the pituitary, the intestinal mucosa and the pancreas. Also found in the brain.
  • 数据库链接：

    KEGG: bta:281070

    STRING: 9913.ENSBTAP00000012973

    UniGene: Bt.49630