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  4. Recombinant Bovine Caspase-3 (CASP3)

Recombinant Bovine Caspase-3 (CASP3)

  • 货号：
    CSB-YP600954BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP600954BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP600954BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP600954BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP600954BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CASP3Caspase-3; CASP-3; EC 3.4.22.56) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    29-175
  • 氨基酸序列
    SG ISLEESYKMD YPEMGLCIII NNKNFHENTG MACRSGTDVD AANLRETFMN LKYEVRIKND LTCKEMLELM SNVSKEDHSK RSSFICVLLS HGEEGIIFGT NGPVNLKKLA SFFRGDYCRS LTGKPKLFII QACRGTELDC GIETD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface.
  • 基因功能参考文献：
    1. Over-expression of STC-1 up-regulated Bcl-2 protein expression and slightly down-regulated caspase-3 production in the damaged cells. Findings from this study suggest that STC-1 plays a protective role in intestinal cells through an antioxidant mechanism. PMID: 24962416
    2. Data suggest that Escherichia coli infections (here, administration of LPS) provokes luteolysis in diestrus, non-lactating cows and up-regulation of expression of caspase 3 in corpus luteum but has no effect on luteinization in the following cycle. PMID: 22829687
    3. Data suggest that activation of caspase-3 is likely involved in both spontaneous and induced apoptosis in bovine pre-implantation embryos. PMID: 17393434
    4. mRNA expression of Bax, Bcl-2, caspase-3 and-7 cannot be used as a reliable apoptosis detection method. PMID: 18272300
    5. the capacity for blastocysts to undergo further development is related to degree of group II caspase activity PMID: 18277054
    6. Treatment of mammary epithelial cells with serotonin 1B and serotonin 2A receptor antagonists increased caspase-3 activity. PMID: 19654143

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase C14A family
  • 数据库链接：

    KEGG: bta:408016

    STRING: 9913.ENSBTAP00000021099

    UniGene: Bt.10084