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  4. Recombinant Bothrops asper Phospholipase A2 homolog 2

Recombinant Bothrops asper Phospholipase A2 homolog 2

  • 中文名称：
    Recombinant Bothrops asper Phospholipase A2 homolog 2,Yeast
  • 货号：
    CSB-YP333003BUT
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Bothrops asper Phospholipase A2 homolog 2,Yeast
  • 货号：
    CSB-EP333003BUT
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Bothrops asper Phospholipase A2 homolog 2,Yeast
  • 货号：
    CSB-EP333003BUT-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Bothrops asper Phospholipase A2 homolog 2,Yeast
  • 货号：
    CSB-BP333003BUT
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Bothrops asper Phospholipase A2 homolog 2,Yeast
  • 货号：
    CSB-MP333003BUT
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    Basic phospholipase A2 homolog 2; svPLA2 homolog; Myotoxin II; Basp-II; Lys49 Mt-II; MtxII
  • 种属：
    Bothrops asper (Terciopelo)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    17-137
  • 氨基酸序列
    SLFE LGKMILQETG KNPAKSYGAY GCNCGVLGRG KPKDATDRCC YVHKCCYKKL TGCNPKKDRY SYSWKDKTIV CGENNSCLKE LCECDKAVAI CLRENLNTYN KKYRYYLKPL CKKADAC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Snake venom phospholipase A2 homolog that lacks enzymatic activity. Is myotoxic and induces a dose-dependent edema in the mouse foot pad. Also exhibits strong anticoagulant effects by binding to factor Xa (F10) and inhibiting the prothrombinase activity (IC(50) is 3 nM). In addition, it shows cytotoxic activity to a variety of cell types and bactericidal activity to a variety of Gram-negative and Gram-positive bacteria. Also induces a very rapid release of large amounts of potassium ions and ATP from muscle cells, which accounts for the pain reaction characteristic of viperid envenomations. The released ATP amplifies the effect of the myotoxins, acting as a 'danger signal', which spreads and causes further damage by acting on purinergic receptors. A model of myotoxic mechanism has been proposed: an apo Lys49-PLA2 is activated by the entrance of a hydrophobic molecule (e.g. fatty acid) at the hydrophobic channel of the protein leading to a reorientation of a monomer. This reorientation causes a transition between 'inactive' to 'active' states, causing alignment of C-terminal and membrane-docking sites (MDoS) side-by-side and putting the membrane-disruption sites (MDiS) in the same plane, exposed to solvent and in a symmetric position for both monomers. The MDoS region stabilizes the toxin on membrane by the interaction of charged residues with phospholipid head groups. Subsequently, the MDiS region destabilizes the membrane with penetration of hydrophobic residues. This insertion causes a disorganization of the membrane, allowing an uncontrolled influx of ions (i.e. calcium and sodium), and eventually triggering irreversible intracellular alterations and cell death.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Phospholipase A2 family, Group II subfamily, K49 sub-subfamily
  • 组织特异性：
    Expressed by the venom gland.