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  4. Recombinant Borrelia burgdorferi Outer surface protein A (ospA)

Recombinant Borrelia burgdorferi Outer surface protein A (ospA)

  • 中文名称：
    Recombinant Borrelia burgdorferi Outer surface protein A(ospA),Yeast
  • 货号：
    CSB-YP317257BUD
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Borrelia burgdorferi Outer surface protein A(ospA),Yeast
  • 货号：
    CSB-EP317257BUD
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Borrelia burgdorferi Outer surface protein A(ospA),Yeast
  • 货号：
    CSB-EP317257BUD-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Borrelia burgdorferi Outer surface protein A(ospA),Yeast
  • 货号：
    CSB-BP317257BUD
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Borrelia burgdorferi Outer surface protein A(ospA),Yeast
  • 货号：
    CSB-MP317257BUD
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ospA
  • Uniprot No.：
  • 别名：
    ospA; BB_A15; Outer surface protein A
  • 种属：
    Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    17-273
  • 氨基酸序列
    CKQN VSSLDEKNSV SVDLPGEMKV LVSKEKNKDG KYDLIATVDK LELKGTSDKN NGSGVLEGVK ADKSKVKLTI SDDLGQTTLE VFKEDGKTLV SKKVTSKDKS STEEKFNEKG EVSEKIITRA DGTRLEYTGI KSDGSGKAKE VLKGYVLEGT LTAEKTTLVV KEGTVTLSKN ISKSGEVSVE LNDTDSSAAT KKTAAWNSGT STLTITVNSK KTKDLVFTKE NTITVQQYDS NGTKLEGSAV EITKLDEIKN ALK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Induces host (human and mouse) cytokine release by monocyte cell lines via TLR2 and CD14; nonlipidated protein does not stimulate host cells.
  • 基因功能参考文献：
    1. Evaluation of OspA vaccination-induced serological correlates of protection against Lyme borreliosis in a mouse model. PMID: 24260146
    2. neuroinvasive Borrelia and its recombinant OspA activated CD40-dependent pathway in BMECs and induced expression of integrins essential for stationary adhesion PMID: 22355605
    3. Calmodulin fusion to truncated or mutant OspA N-terminal tether peptides were targeted to the periplasm. PMID: 21965569
    4. Both OspA and Vsp1 are compatible in anchoring these surface lipoproteins in the outer leaflet of the outer spirochetal membrane. PMID: 21908659
    5. data show disturbance of confined tether feature leads to premature folding of OspA in periplasm and prevents secretion through the membrane;suggest OspA emerges tail-first on bacterial surface independent of specific C-terminal targeting sequence PMID: 20398211
    6. The expression of OspA and OspC in B. burgdorferi in human tissue culture. PMID: 15838803
    7. the interaction between OspA and human complement receptor 3 is direct and not complement 3b-mediated PMID: 16113335
    8. The OspA single-layer beta-sheet achieves efficient hydrophobic surface burial without forming a hydrophobic core by a strategic placement of a variety of side-chains. PMID: 17335845
    9. The host neuroendocrine stress hormones, epinephrine and norepinephrine, are specifically bound by B. burgdorferi and result in increased expression of OspA. PMID: 17438273
    10. There remains an association between OspA(165-173) and antibiotic-refractory Lyme arthritis, and infection-induced autoimmunity is an attractive hypothesis to explain this outcome. PMID: 17555819
    11. OspB inhibits the phagocytosis and oxidative burst of human neutrophils at low serum concentrations, whereas OspA induces the oxidative burst in neutrophils. PMID: 18068388
    12. Data did not find any evidence for glycosylation of the major outer membrane proteins OspA. PMID: 18245287
    13. Increasing expression of OspA fully protected the ospC mutant from elimination from the skin tissue of SCID mice. PMID: 18452586
    14. OspA serves a critical antibody-shielding role during vector blood meal uptake from immune hosts and is not required for persistence in the tick vector. PMID: 18779341
    15. Increasing production of OspA or VlsE influences lipoprotein gene expression in the murine host and alters the infectious behaviour of B. burgdorferi. PMID: 18957595

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  • 亚细胞定位：
    Cell outer membrane; Lipid-anchor.
  • 数据库链接：

    KEGG: bbu:BB_A15