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  4. Recombinant Bacillus subtilis Transcriptional regulatory protein YycF (yycF)

Recombinant Bacillus subtilis Transcriptional regulatory protein YycF (yycF)

  • 中文名称：
    枯草芽孢杆菌walR重组蛋白
  • 货号：
    CSB-YP330717BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    枯草芽孢杆菌walR重组蛋白
  • 货号：
    CSB-EP330717BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    枯草芽孢杆菌walR重组蛋白
  • 货号：
    CSB-EP330717BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    枯草芽孢杆菌walR重组蛋白
  • 货号：
    CSB-BP330717BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    枯草芽孢杆菌walR重组蛋白
  • 货号：
    CSB-MP330717BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    walR
  • Uniprot No.：
  • 别名：
    walR; yycF; BSU40410; Transcriptional regulatory protein WalR
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-235
  • 氨基酸序列
    MDKKILVVDD EKPIADILEF NLRKEGYEVH CAHDGNEAVE MVEELQPDLI LLDIMLPNKD GVEVCREVRK KYDMPIIMLT AKDSEIDKVI GLEIGADDYV TKPFSTRELL ARVKANLRRQ LTTAPAEEEP SSNEIHIGSL VIFPDAYVVS KRDETIELTH REFELLHYLA KHIGQVMTRE HLLQTVWGYD YFGDVRTVDV TVRRLREKIE DNPSHPNWIV TRRGVGYYLR NPEQD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Member of the two-component regulatory system WalK/WalR involved in the regulation of the ftsAZ operon, the yocH, ykvT, cwlO, lytE, ydjM, yjeA, yoeB genes and the tagAB and tagDEF operons. Binds to the ftsAZ P1 promoter sequence in vitro. WalR has been shown to directly bind to the regulatory regions of yocH, ykvT, tagAB/tagDEF. Activates cwlO, lytE and ydjM and represses yoeB and yjeA.
  • 基因功能参考文献：
    1. the regulatory link between WalRK and sigma(I) RsgI expression ensures that the endopeptidase requirement (CwlO or LytE) for cell viability is fulfilled during growth and under stress conditions. PMID: 23199363
    2. The data indicate that YycG activity in non-dividing cells is suppressed by its interaction with YycH and YycI and its activation is co-ordinated to cell division in dividing cells by specific interactions that occur within the divisome. PMID: 21219466
    3. the essential genes involved in growth of B. subtilis appear to be regulated by the homodimer of YycF PMID: 15153768
    4. A genetic analysis shows that YycFG essentiality is polygenic in nature, being a manifestation of disrupted cell wall metabolism caused by aberrant expression of a number of YycFG regulon genes. PMID: 17581128
    5. The X-ray crystal structure of the effector domain of Bacillus subtilis YycF involved in DNA binding was determined. PMID: 18789936

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  • 亚细胞定位：
    Cytoplasm.
  • 数据库链接：

    KEGG: bsu:BSU40410

    STRING: 224308.Bsubs1_010100021806