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  4. Recombinant Bacillus subtilis Repressor rok (rok)

Recombinant Bacillus subtilis Repressor rok (rok)

  • 货号：
    CSB-YP522016BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP522016BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP522016BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP522016BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP522016BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rok
  • Uniprot No.：
  • 别名：
    rok; ykuW; BSU14240; Repressor Rok
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-191
  • 氨基酸序列
    MFNEREALRL RLEQLNEAEV KVIREYQIER DKIYAKLREL DRNGSPSEIK KDFRSEKKPD SLPVLAELAA QEIRSYQPQS QQQSVQPQLQ SISSLPAGIP DGTTRRRRGT ARPGSKAAKL REAAIKTLKR HNAAIKSSEL QKEIEKESGL EIPNMTTFMQ SLIKMYPEVK KPYRGQYILE GEIESAESAN E
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Repressor of comK, the master regulator of competence development. Overexpression seems to be lethal. Represses at least 20 genes that specify membrane-localized and secreted proteins, including some that encode products with antibiotic activity. Binds to many AT-rich sites in the chromosome, many of which are known or thought to derive from horizontal gene transfer; helps keep mobile element ICEBs1 quiescent in the genome. Binds to its own promoter and is thus probably autoregulatory.
  • 基因功能参考文献：
    1. he large conjugative B. subtilis plasmid pLS20 harbours a rok homologue rok(LS20)and its ectopic expression leads to inhibition of competence by repressing comK PMID: 22779408
    2. Rok and YkrK are repressors of ykrL expression. PMID: 22447908
    3. Rok regulates yuaB expression during colony development. PMID: 21097620
    4. Rok is a nucleoid associated protein that serves to help repress expression of A+T-rich genes. PMID: 21085634
    5. evidence for the recent introduction of rok into the B. subtilis-Bacillus licheniformis-Bacilllus amyloliquefaciens group by horizontal transmission. PMID: 15743949

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  • 亚细胞定位：
    Cytoplasm, nucleoid.
  • 数据库链接：

    KEGG: bsu:BSU14240

    STRING: 224308.Bsubs1_010100007906