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  4. Recombinant Arabidopsis thaliana Small RNA degrading nuclease 1 (SDN1)

Recombinant Arabidopsis thaliana Small RNA degrading nuclease 1 (SDN1)

  • 中文名称：
    拟南芥SDN1重组蛋白
  • 货号：
    CSB-YP385279DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥SDN1重组蛋白
  • 货号：
    CSB-EP385279DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥SDN1重组蛋白
  • 货号：
    CSB-EP385279DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥SDN1重组蛋白
  • 货号：
    CSB-BP385279DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥SDN1重组蛋白
  • 货号：
    CSB-MP385279DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SDN1
  • Uniprot No.：
  • 别名：
    SDN1; At3g50100; F3A4.180; Small RNA degrading nuclease 1; EC 3.1.-.-
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-409
  • 氨基酸序列
    MELKLATAEK QVLDELVKLL QSRDLRGENG NWKEFLHVYD KNADSPSDPS RRSHEDLVQF LTTFKKKEDL QLLKCHANHL LIENLKQESQ DEDTPEQMLV RLTVEHPSYS LDYSFKPYSE DWFVSDVGMK MKKVMESTNM VAVDCEMVLC EDGTEGLVRV GVVDRDLKVI LDEFVKPNKP VVDYRTDITG ITAEDIENAS LSVVDIQETL QPFLSTGTIL VGHSLNRDLE VLKIDHPKVI DTALVFKYPN TRKLRRPSLN NLCKSILGYE VRKTGVPHDC VHDASAAMKL ALAVVEKRVD TTIKPSKEML EVEKAKLFLH KIPNNVPSEE LEQVLSGKFT LDVKQAKTQG RYYCAFALFH SSEDADQAFE HIDGIEMTDS LGLPQKVVII KLSSGSRASI YVRKMVQDE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    3'-5' exonuclease degrading single-stranded small RNAs.
  • 基因功能参考文献：
    1. The SDN1 C-terminal domain adopts an RNA recognition motif fold and is critical for substrate binding and enzymatic processivity of SDN1.SDN1 binds AGO protein PAZ domain and trims the 3' ends of AGO-bound miRNAs. PMID: 30181559
    2. AGO10 reduces miR165/6 accumulation by enhancing its degradation by SDN1 and SDN2 in vivo. PMID: 28231321
    3. findings show that a family of exoribonucleases encoded by SMALL RNA DEGRADING NUCLEASE genes degrades mature miRNAs; SDN1 acts specifically on single-stranded miRNAs in vitro & is sensitive to 2'-O-methyl modification on the 3' terminal ribose of miRNAs PMID: 18787168
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    REXO1/REXO3 family
  • 数据库链接：

    KEGG: ath:AT3G50100

    STRING: 3702.AT3G50100.1

    UniGene: At.35533