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  4. Recombinant Arabidopsis thaliana Receptor-like protein kinase FERONIA (FER), partial

Recombinant Arabidopsis thaliana Receptor-like protein kinase FERONIA (FER), partial

  • 中文名称：
    拟南芥FER重组蛋白
  • 货号：
    CSB-YP871036DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥FER重组蛋白
  • 货号：
    CSB-EP871036DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥FER重组蛋白
  • 货号：
    CSB-EP871036DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥FER重组蛋白
  • 货号：
    CSB-BP871036DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥FER重组蛋白
  • 货号：
    CSB-MP871036DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    FER
  • Uniprot No.：
  • 别名：
    FER; AAK1; SIR; SRN; At3g51550; F26O13.190Receptor-like protein kinase FERONIA; EC 2.7.11.1; Protein SIRENE
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor-like protein kinase that mediates the female control of male gamete delivery during fertilization, including growth cessation of compatible pollen tubes ensuring a reproductive isolation barriers, by regulating MLO7 subcellular polarization upon pollen tube perception in the female gametophyte synergids. Required for cell elongation during vegetative growth, mostly in a brassinosteroids- (BR-) independent manner. Acts as an upstream regulator for the Rac/Rop-signaling pathway that controls ROS-mediated root hair development. Seems to regulate a cross-talk between brassinosteroids and ethylene signaling pathways during hypocotyl elongation. Negative regulator of brassinosteroid response in light-grown hypocotyls, but required for brassinosteroid response in etiolated seedlings. Mediates sensitivity to powdery mildew (e.g. Golovinomyces orontii). Positive regulator of auxin-promoted growth that represses the abscisic acid (ABA) signaling via the activation of ABI2 phosphatase. Required for RALF1-mediated extracellular alkalinization in a signaling pathway preventing cell expansion.
  • 基因功能参考文献：
    1. suppression of RALF1 gene expression, similar to disruption of the FER gene, rendered plants hypersensitive to ABA. These results formulated a mechanism for ABA activation of FER and for cross-talk between ABA and peptide hormone RALF in the control of plant growth and responses to stress signals PMID: 27566404
    2. LRE and FERONIA jointly function in pollen tube reception at the interface of the synergid cell and pollen tube. PMID: 27081182
    3. FER acts as a RALF-regulated scaffold that modulates receptor kinase complex assembly. A similar scaffolding mechanism may underlie FER function in other signaling pathways. PMID: 28104890
    4. The results of these experiments are consistent with a model in which kinase-mediated phosphorylation within the C-terminal region is inhibitory and regulates catalytic activity. These data represent a step further toward understanding the molecular basis for the protein kinase catalytic activity of FERONIA and show promise for future characterization of eukaryotic membrane proteins. PMID: 28235802
    5. We propose that FER receptor kinase, in response to environmental stress and plant hormones such as auxin and brassinolide, interacts with S-adenosylmethionine (SAM) synthases and down-regulates ethylene biosynthesis. PMID: 25988356
    6. Glycosylphosphatidylinositol-anchored proteins LLG1and LORELEI act as necessary chaperones and co-receptors for FERONIA receptor kinase signaling in Arabidopsis. PMID: 26052747
    7. An Arabidopsis mutant lacking the receptor-like kinase FERONIA shows severely altered Ca(2+) signaling and growth responses to different forms of mechanical perturbation. Ca(2+) signals are either abolished or exhibit qualitatively different signatures. PMID: 25127214
    8. Three independent T-DNA insertion lines for FERONIA and its pollen-specific homolog ANXUR2 showing deformed pollen phenotypes caused by chromosomal translocations, were identified. PMID: 24874868
    9. FER controls seed size in Arabidopsis thaliana PMID: 24482438
    10. a secreted peptide, RALF (rapid alkalinization factor), suppresses cell elongation of the primary root by activating the cell surface receptor FERONIA in Arabidopsis thaliana. PMID: 24458638
    11. FERONIA receptor kinase pathway suppresses abscisic acid signaling in Arabidopsis by activating ABI2 phosphatase. PMID: 22908257
    12. These findings position the THESEUS 1/FERONIA family RLKs as surface regulators and potential cell wall sensors capable of broadly and profoundly impacting cellular pathways in response to diverse signals. PMID: 21963060
    13. study shows pollen tube (PT) reception and powdery mildew (PM) infection share molecular components; NORTIA, discovered in context of PM resistance, and FER control PT reception; fer mutants also display PM resistance revealing a new function for FER PMID: 21071669
    14. FER is an upstream regulator for the RAC/ROP-signaled pathway that controls ROS-mediated root hair development. PMID: 20876100
    15. Pollen tube discharge is controlled by an interaction between the synergids expressing SRN/FER and the central cell expressing FIS genes. PMID: 19825570
    16. FER protein accumulates asymmetrically in the synergid membrane at the filiform apparatus; findings show that female control of pollen tube reception is based on a FER-dependent signaling pathway, which may play a role in reproductive isolation barriers PMID: 17673660
    17. 3 related receptor-like kinases, HERCULES1, THESEUS1, and FERONIA, transcriptionally induced by brassinosteroids are charcterized in this study. PMID: 19383785
    18. Disruption of the pollen-expressed FERONIA homologs ANXUR1 and ANXUR2 triggers pollen tube discharge PMID: 19736323

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Note=Accumulates asymmetrically in the female gametophyte synergid membrane at the filiform apparatus.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 组织特异性：
    Expressed in leaves, buds, flowers, siliques, young ovules primordia, and young anthers with immature pollen, but not detected in mature pollen. Highest expression in the synergid cells of the female gametophyte.
  • 数据库链接：

    KEGG: ath:AT3G51550

    STRING: 3702.AT3G51550.1

    UniGene: At.656