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  4. Recombinant Arabidopsis thaliana Profilin-1 (PRO1)

Recombinant Arabidopsis thaliana Profilin-1 (PRO1)

  • 货号：
    CSB-YP677077DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP677077DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP677077DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP677077DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP677077DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PRO1
  • Uniprot No.：
  • 别名：
    PRF1; PFN1; PRO1; At2g19760; F6F22.21; Profilin-1; AtPROF1; AthPRF1; allergen Ara t 8
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-131
  • 氨基酸序列
    SWQSYVDDH LMCDVEGNHL TAAAILGQDG SVWAQSAKFP QLKPQEIDGI KKDFEEPGFL APTGLFLGGE KYMVIQGEQG AVIRGKKGPG GVTIKKTNQA LVFGFYDEPM TGGQCNLVVE RLGDYLIESE L
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to actin monomers and regulates the organization of the actin cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. At low concentrations, associates with the poly-proline motif of formins to enhance actin filament elongation rate. Binds ACT1, ACT7 and ACT11 and inhibits actin polymerization. Coordinates the stochastic dynamic properties of actin filaments by modulating formin-mediated actin nucleation and assembly during axial cell expansion. Binds G-actin and poly-L-proline in vitro. Inhibits cell growth of various pathogenic fungal strains. May play a role as antifungal proteins in the defense system against fungal pathogen attacks.
  • 基因功能参考文献：
    1. PRF1 coordinates the stochastic dynamic properties of actin filaments by modulating formin-mediated actin nucleation and assembly during plant cell expansion. PMID: 26574597
    2. formed a filamentous network likely associated with actin filaments PMID: 19200149
  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm.
  • 蛋白家族：
    Profilin family
  • 组织特异性：
    Expressed at low levels roots, leaves, stems, flowers and siliques. Expressed in leaf epidermal cells, trichomes and stem epidermal cells. Detected in phloem exudates (at protein level).
  • 数据库链接：

    KEGG: ath:AT2G19760

    STRING: 3702.AT2G19760.1

    UniGene: At.23712