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  4. Recombinant Arabidopsis thaliana Mitogen-activated protein kinase 6 (MPK6)

Recombinant Arabidopsis thaliana Mitogen-activated protein kinase 6 (MPK6)

  • 货号：
    CSB-YP013458DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP013458DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP013458DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP013458DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP013458DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MPK6
  • Uniprot No.：
  • 别名：
    MPK6; At2g43790; F18O19.10; Mitogen-activated protein kinase 6; AtMPK6; MAP kinase 6; EC 2.7.11.24
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-395
  • 氨基酸序列
    MDGGSGQPAA DTEMTEAPGG FPAAAPSPQM PGIENIPATL SHGGRFIQYN IFGNIFEVTA KYKPPIMPIG KGAYGIVCSA MNSETNESVA IKKIANAFDN KIDAKRTLRE IKLLRHMDHE NIVAIRDIIP PPLRNAFNDV YIAYELMDTD LHQIIRSNQA LSEEHCQYFL YQILRGLKYI HSANVLHRDL KPSNLLLNAN CDLKICDFGL ARVTSESDFM TEYVVTRWYR APELLLNSSD YTAAIDVWSV GCIFMELMDR KPLFPGRDHV HQLRLLMELI GTPSEEELEF LNENAKRYIR QLPPYPRQSI TDKFPTVHPL AIDLIEKMLT FDPRRRITVL DALAHPYLNS LHDISDEPEC TIPFNFDFEN HALSEEQMKE LIYREALAFN PEYQQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Mitogen-activated protein kinase (MAPK) which regulates abscisic acid (ABA) responses in a MAPKKK20-MKK5-MPK6 cascade involved in root growth (e.g. root cell division and elongation) and stomatal response. Involved in oxidative stress-mediated signaling cascade (such as ozone). Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. May be involved in hypersensitive response (HR)-mediated signaling cascade by modulating LIP5 phosphorylation and subsequent multivesicular bodies (MVBs) trafficking. May phosphorylate regulators of WRKY transcription factors. Phosphorylates 1-aminocyclopropane-1-carboxylic acid synthases (ACS2 and ACS6) and may be involved in the regulation of bacterial elicitor flagellin-induced ethylene production. Regulates locally gene-mediated and basal resistance response to certain pathogens. May be involved in the cold and salinity stress-mediated MAP kinase signaling cascade (MEKK1, MKK1/MKK2 and MPK4/MPK6). MKK1-MPK6 module mediates abscisic acid (ABA)-dependent CAT1 expression with H(2)O(2) production and response to drought and salt stress. MKK1-MPK6 module is also involved in sugar signaling during the process of seed germination. MKK3-MPK6 module plays an important role in the jasmonate signal transduction pathway through the negative regulation of MYC2/JIN1 expression. MKK9-MPK3/MPK6 module phosphorylates and activates EIN3, leading to the promotion of EIN3-mediated transcription in ethylene signaling. MPK3/MPK6 cascade regulates camalexin synthesis through transcriptional regulation of the biosynthetic genes after pathogen infection. MKK9-MPK6 module positively regulates leaf senescence. YDA-MKK4/MKK5-MPK3/MPK6 module regulates stomatal cell fate before the guard mother cell (GMC) is specified. When activated, reinforces the feedback loop by phosphorylating BASL, and inhibits stomatal fate by phosphorylating SPCH. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs.
  • 基因功能参考文献：
    1. The Arabidopsis MKK4/MKK5-MPK6 cascade is an important player in the maternal control of embryogenesis. PMID: 29024034
    2. abscisic acid and jasmonate mediate inactivation of the immune-associated MAP kinases (MAPKs), MPK3 and MPK6, in Arabidopsis thaliana ABA induced expression of genes encoding the protein phosphatases 2C (PP2Cs), HAI1, HAI2, and HAI3 through ABF/AREB transcription factors PMID: 28652328
    3. Proteome changes associated with MPK4 and MPK6 deficiency in Arabidopsis roots. PMID: 27324189
    4. Results suggest that these mutants can be used to analyze the specific biological functions of MAP kinase 6 (MPK6). PMID: 27160427
    5. These data highlight MPK6 and MKP1 as components of an L-Glutamate pathway linking the auxin response, and cell division for primary root growth. PMID: 29344832
    6. Genetic and biochemical evidences not only show that MEK1-MPK6 cascade, AtRBOHD/F-dependent H2O2 and NIA1-dependent NO are all involved in dark-induced stomatal closure in Arabidopsis, also indicate that MEK1-MPK6 cascade functions via working downstream of H2O2 and upstream of NO. PMID: 28716416
    7. that MPK3/MPK6 phosphorylate and destabilize ICE1, which negatively regulates CBF expression and freezing tolerance in plants PMID: 29056553
    8. BASL polarization leads to elevated nuclear MPK6 signaling and lowered SPCH abundance in one of the two daughter cells. Therefore, two daughter cells are differentiated by BASL polarity-mediated differential suppression of SPCH, which may provide developmental plasticity in plant stem cell asymmetric cell division. PMID: 27746029
    9. results indicated that AIK1-MKK5-MPK6 functions in ABA responses and requires ABA-responsive gene expression to regulate ABA-inhibited root growth and cell division. The ABA signaling pathway regulates this MAPK cascade. PMID: 28494202
    10. MPK6 role in ultraviolet induced stomatal closure PMID: 27837091
    11. Study propose that the pathogen-responsive MPK3/MPK6 cascade and ABA are two essential signaling pathways that control, respectively, the organic acid metabolism and ion channels, two main branches of osmotic regulation in guard cells that function interdependently to control stomatal opening/closure. PMID: 28254778
    12. MPK6-mediated regulation of MYB15 plays an important role in cold stress signaling in Arabidopsis. PMID: 28510716
    13. Data report that MPK3/MPK6 and their substrate ERF6 promote the biosynthesis of IGSs and the conversion of I3G to 4MI3G, a target of PEN2/PEN3-dependent chemical defenses in plant immunity. PMID: 27081184
    14. Data show that the protein kinases MPK3 and MPK6 can both interact with SPOROCYTELESS/NOZZLE (SPL) in vitro and in vivo and can phosphorylate the SPL protein in vitro. PMID: 28209842
    15. AIK1-MKK5-MPK6 cascade functions in the abscisic acid regulation of primary root growth and stomatal response. PMID: 27913741
    16. A novel mechanism for PIN1 phosphorylation involving MKK7 and MPK6 in shoot branching regulation in Arabidopsis. PMID: 27618482
    17. MKK4, MKK5, MKK7, and MKK9, are responsible for the activation of MPK3 and MPK6 by melatonin, indicating that melatonin-mediated innate immunity is triggered by MAPK signaling through MKK4/5/7/9-MPK3/6 cascades. PMID: 26927635
    18. Mitogen-activated protein kinase 6 (MPK6) promoted C-terminal end of ORE3/EIN2 (CEND cleavage and nuclear translocation. Nuclear CEND accumulated ETHYLENE INSENSITIVE3 (EIN3), a transcription factor that accelerates MeJA-induced leaf senescence. PMID: 26507893
    19. Results demonstrated the contribution of MPK3 and MPK6 to riboflavin-induced resistance. PMID: 27054585
    20. MPK6 phosphorylates EB1c, but not EB1a, and has a role in maintaining regular planes of cell division under stress conditions. PMID: 26061286
    21. BES1 is identified as a unique direct substrate of MPK6 in Pathogen-associated molecular patterns (PAMP)-triggered immunity signaling. PMID: 25609555
    22. These results indicate that the MVB pathway is positively regulated by pathogen-responsive MPK3/6 through LIP5 phosphorylation and plays a critical role in plant immune system PMID: 25010425
    23. Ethylene (ET) affects auxin signaling through the central regulator CTR1 and that fungal-derived compounds, such as indole-3-acetic acid and indole-3-acetaldehyde, induce MPK6 activity. PMID: 26067203
    24. AGB1 interacts with AtMPK6 and may negatively regulate the abscissic acid signaling pathway. PMID: 25635681
    25. MPK6 controls hydrogen peroxide-induced root elongation by mediating Ca2+ influx across the plasma membrane of root cells in Arabidopsis seedlings. PMID: 25145265
    26. MPK6-mediated WRKY6 and Trx h5 transcriptional activation co-regulated the expression of the NPR1 gene and the monomerization of NPR1 protein, allowing it to enter the nucleus, thereby promoting salicylic acid-induced leaf senescence. PMID: 25210078
    27. MPK3 and MPK6 target a subclass of 'VQ-motif'-containing proteins to regulate immune responses. PMID: 24750137
    28. These results suggest that the MKK9-MPK3/MPK6 cascade is part of the phosphate signaling pathway in plants. PMID: 24865627
    29. YODA and MPK6 are involved in post-embryonic root development through an auxin-dependent mechanism regulating cell division and mitotic microtubule (PPB and phragmoplast) organization. PMID: 24923680
    30. retrograde signal transmission model is proposed starting with metabolite export through the triose phosphate/phosphate translocator with subsequent MPK6 activation leading to initiation of AP2/ERF-TF gene expression PMID: 24668746
    31. HSFA4A is a substrate of the MPK3/MPK6 signaling and that it regulates stress responses in Arabidopsis. PMID: 24676858
    32. establishment of an mpk3 mpk6 double mutant system using a chemical genetic approach provides a powerful tool to investigate the function of MPK3/MPK6 in the plant defense signaling pathway PMID: 24245741
    33. Loss of MPK3/MPK6 phosphorylation sites in WRKY34 compromises the function of WRKY34 in vivo. Epistasis interaction analysis confirmed that MPK6 belongs to the same genetic pathway of WRKY34 and WRKY2 PMID: 24830428
    34. MPK6 plays an important role during embryo development and acts as a repressor of primary and lateral root development. PMID: 24218326
    35. mpk6 decreased NaCl-induced the Na(+) accumulation by modifying plasma membrane (PM) Na(+) flux in root cells. There was a NaCl-induced increase of cytosolic Ca(2+) in mpk6 root cells, resulting from PM Ca(2+) influx. PMID: 24484955
    36. MPK3/MPK6 regulate multiple defense pathways in plant fungal resistance. PMID: 23524660
    37. These results suggest that MPK6 functions in both penetration and post-penetration resistance to M. oryzae in Arabidopsis thaliana. PMID: 23748771
    38. These data might provide new insight into the mechanisms that regulate reactive oxygen species-responsive gene transcription via a complex of MPK6, ERF6, and the ROSE7/GCC box under oxidative stress. PMID: 23300166
    39. these results suggest that phosphorylation by MPK6 is required for the functional role of ZAT6 in seed germination under salt and osmotic stress. PMID: 23257164
    40. The role of AtBI1 over-expression in delaying Methyl jasmonate-induced leaf senescence by suppressing the [Ca(2+)](cyt)-dependent activation of MPK6, thus providing a new insight into the function and mechanism of AtBI1 in plant senescence. PMID: 22563118
    41. These results suggest that ROS, but not abscisic acid, induces MPK6 activation as an upstream signal under drought conditions. PMID: 22975351
    42. A mpk6, but not a mpk3, null allele is able to suppress the effects of this dominant WAK2 mutation PMID: 22155845
    43. Data suggest that amino acids surrounding the phosphorylated serine residue of peptide substrates contribute to MPK6/MPK3 substrate specificity; MPK6/MPK3 activities appear to be important in growth of plant stomata. PMID: 22631074
    44. MAP Kinase 6-mediated activation of vacuolar processing enzyme modulates heat shock-induced programmed cell death in Arabidopsis PMID: 22497243
    45. These results indicate that the phosphorylation of MYB41 by MPK6 is required for the biological function of MYB41 in salt tolerance. PMID: 22575450
    46. The change in transcript profile of MAP2K9 and MAPK6 in leaves of A. thaliana ecotpe Columbia at early, middle and late stages of Alternaria blight infection, were determined. PMID: 21947882
    47. AtMPK6 activation was also triggered by phytosphingosine-1-phosphate (PHS-P) treatment independently of phytosphingosine/PHS-P balance. PMID: 22236066
    48. These results suggest that mRNA decapping through MPK6-DCP1-DCP5 pathway serves as a rapid response to dehydration stress in Arabidopsis. PMID: 22407295
    49. MKKK20 involved in the response to various abiotic stresses, especially osmotic stress, through its regulation of MPK6 activity PMID: 21969089
    50. MPK3 and MPK6 are negative regulators of the acute UV-B stress-resistance pathway, and are regulated by MKP1 in a UVR8-independent manner. PMID: 21790814

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cytoplasm, cell cortex.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
  • 数据库链接：

    KEGG: ath:AT2G43790

    STRING: 3702.AT2G43790.1

    UniGene: At.22266