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  4. Recombinant Arabidopsis thaliana Glutathione gamma-glutamylcysteinyltransferase 1 (PCS1)

Recombinant Arabidopsis thaliana Glutathione gamma-glutamylcysteinyltransferase 1 (PCS1)

  • 中文名称：
    拟南芥PCS1重组蛋白
  • 货号：
    CSB-YP879683DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥PCS1重组蛋白
  • 货号：
    CSB-EP879683DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥PCS1重组蛋白
  • 货号：
    CSB-EP879683DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥PCS1重组蛋白
  • 货号：
    CSB-BP879683DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥PCS1重组蛋白
  • 货号：
    CSB-MP879683DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PCS1
  • Uniprot No.：
  • 别名：
    PCS1; ARA8; CAD1; At5g44070; MRH10.11; MRH10_18Glutathione gamma-glutamylcysteinyltransferase 1; EC 2.3.2.15; Cadmium tolerance protein; Phytochelatin synthase 1; AtPCS1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-485
  • 氨基酸序列
    MAMASLYRRS LPSPPAIDFS SAEGKLIFNE ALQKGTMEGF FRLISYFQTQ SEPAYCGLAS LSVVLNALSI DPGRKWKGPW RWFDESMLDC CEPLEVVKEK GISFGKVVCL AHCSGAKVEA FRTSQSTIDD FRKFVVKCTS SENCHMISTY HRGVFKQTGT GHFSPIGGYN AERDMALILD VARFKYPPHW VPLKLLWEAM DSIDQSTGKR RGFMLISRPH REPGLLYTLS CKDESWIEIA KYLKEDVPRL VSSQHVDSVE KIISVVFKSL PSNFNQFIRW VAEIRITEDS NQNLSAEEKS RLKLKQLVLK EVHETELFKH INKFLSTVGY EDSLTYAAAK ACCQGAEILS GSPSKEFCCR ETCVKCIKGP DDSEGTVVTG VVVRDGNEQK VDLLVPSTQT ECECGPEATY PAGNDVFTAL LLALPPQTWS GIKDQALMHE MKQLISMASL PTLLQEEVLH LRRQLQLLKR CQENKEEDDL AAPAY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants. Also involved in glutathione-conjugates degradation.
  • 基因功能参考文献：
    1. Findings suggest that the PCS1 protein (At5g44070) might be involved in the production of the enzyme phytochelatin synthase, which in turn bind, localize, store or sequester heavy metals in plant cells. PMID: 24554125
    2. Recombinant AtPCS1s with mutations at Tyr55 showed lower catalytic activities because of reduced affinity (3-fold for Y55W) for the CdGS2, further suggesting the role of the cation-pi interaction in recognition of the second substrate. PMID: 24340051
    3. Loss of AtNRAMP3 and AtNRAMP4 function or of PCS1 function leads to comparable Cd sensitivity. PMID: 22998565
    4. Inducible expression of the Arabidopsis phytochelatin synthase AtPCS1 in the triple knockout yeast system, resulted in the turnover of glutathione-S-bimane to the gamma-GluCys-bimane conjugate as observed in plants. [phytochelatin synthase] [AtPCS1] PMID: 19897216
    5. When AtPCS1 was overexpressed in Arabidopsis the A2::AtPCS1 plants were highly resistant to arsenic, accumulating 20-100 times more biomass on 250 and 300 microM arsenate than wild type. PMID: 15653797
    6. Cd(2+) tolerance in cultured rolB roots was increased as a result of overexpression of AtPCS1, and further enhanced when reduced glutathione (GSH, the substrate of PCS1) was added to the culture medium. PMID: 16133212
    7. Arabidopsis PCS1 in the cytosol importantly decreased cadmium tolerance, both cadmium tolerance and accumulation of plants expressing plastidial PCS1 were not significantly affected compared to wild-type. PMID: 16766112

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  • 蛋白家族：
    Phytochelatin synthase family
  • 组织特异性：
    Expressed in roots and shoots.
  • 数据库链接：

    KEGG: ath:AT5G44070

    STRING: 3702.AT5G44070.1

    UniGene: At.7305