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  4. Recombinant Arabidopsis thaliana Floral homeotic protein AGAMOUS (AG)

Recombinant Arabidopsis thaliana Floral homeotic protein AGAMOUS (AG)

  • 中文名称：
    Recombinant Arabidopsis thaliana Floral homeotic protein AGAMOUS(AG),Yeast
  • 货号：
    CSB-YP325945DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Floral homeotic protein AGAMOUS(AG),Yeast
  • 货号：
    CSB-EP325945DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Floral homeotic protein AGAMOUS(AG),Yeast
  • 货号：
    CSB-EP325945DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Floral homeotic protein AGAMOUS(AG),Yeast
  • 货号：
    CSB-BP325945DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Floral homeotic protein AGAMOUS(AG),Yeast
  • 货号：
    CSB-MP325945DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    AG
  • Uniprot No.：
  • 别名：
    AG; At4g18960; F13C5.130Floral homeotic protein AGAMOUS
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-252
  • 氨基酸序列
    MAYQSELGGD SSPLRKSGRG KIEIKRIENT TNRQVTFCKR RNGLLKKAYE LSVLCDAEVA LIVFSSRGRL YEYSNNSVKG TIERYKKAIS DNSNTGSVAE INAQYYQQES AKLRQQIISI QNSNRQLMGE TIGSMSPKEL RNLEGRLERS ITRIRSKKNE LLFSEIDYMQ KREVDLHNDN QILRAKIAEN ERNNPSISLM PGGSNYEQLM PPPQTQSQPF DSRNYFQVAA LQPNNHHYSS AGRQDQTALQ LV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probable transcription factor involved in the control of organ identity during the early development of flowers. Is required for normal development of stamens and carpels in the wild-type flower. Plays a role in maintaining the determinacy of the floral meristem. Acts as C class cadastral protein by repressing the A class floral homeotic genes like APETALA1. Forms a heterodimer via the K-box domain with either SEPALATTA1/AGL2, SEPALATTA2/AGL4, SEPALLATA3/AGL9 or AGL6 that could be involved in genes regulation during floral meristem development. Controls AHL21/GIK, a multifunctional chromatin modifier in reproductive organ patterning and differentiation. Induces microsporogenesis through the activation of SPL/NZZ.
  • 基因功能参考文献：
    1. findings suggest that AGAMOUS functions to maintain the irreversible state of reproductive development through the negative regulation of floral meristem identity genes and genes involved in organ initiation PMID: 29218596
    2. AP2 does not repress the transcription of AG in the inner two whorls, but instead counteracts AG activity. PMID: 27604611
    3. ARF3 integrates the functions of AGAMOUS and APETALA2 in floral meristem determinacy. PMID: 25187180
    4. The ULT1 and LFY pathways act separately in regulating identity and determinacy at the floral meristem. In particular, they independently induce AG expression in the centre of the flower to terminate meristem activity. PMID: 25288633
    5. FLK and PEP physically interact with proteins involved in AG pre-mRNA processing to secure correct AG function in the floral meristem and flower PMID: 25658099
    6. AGAMOUS directly represses WUS expression by binding to the WUS locus and recruiting, directly or indirectly, PcG that methylates histone H3 Lys-27 at WUS. PMID: 22028461
    7. A model in which the decision whether stamens or petals develop is based on the balance between AP2 and AG activities, rather than the two being mutually exclusive. PMID: 20876650
    8. floral phenotype of bellringer mutants is caused by derepression of AG, suggesting that BLR functions as a transcription repressor PMID: 15155890
    9. AG activates biosynthesis of gibberellin, which has been proposed to promote the shift from meristem identity to differentiation PMID: 15634696
    10. AG and miR172 have distinct functions in flower development and that they largely act independently in the negative regulation of AP2. PMID: 17573799
    11. These results indicate that stamen identity and differentiation control by AG is achieved by the regulation of different transcriptional cascades in different floral stages. PMID: 17981996
    12. Transgenic Arabidopsis plants harboring two tissue-specific promoters, fAGIP and rAGIP in forward and reverse orientations, fAGIP::GUS or rAGIP::GUS displayed similar GUS expression specifically in carpel and stamen tissues and their primordial cells. PMID: 18256838
    13. PERIANTHIA (PAN) plays a role in regulating stem cell fate by directly controlling AG expression and suggest that this activity is spatially restricted to the centermost region of the AG expression domain. PMID: 19395638
    14. Upon enhanced central AG expression, the C-domain laterally expands necessitating tuning of the expression level of C-function genes in the wild type. PMID: 19554348

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Detected early in the floral meristem but mostly expressed in stamen and carpel primordia.
  • 数据库链接：

    KEGG: ath:AT4G18960

    STRING: 3702.AT4G18960.1

    UniGene: At.28088