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  4. Recombinant Arabidopsis thaliana CBL-interacting serine/threonine-protein kinase 14 (CIPK14)

Recombinant Arabidopsis thaliana CBL-interacting serine/threonine-protein kinase 14 (CIPK14)

  • 中文名称：
    Recombinant Arabidopsis thaliana CBL-interacting serine/threonine-protein kinase 14(CIPK14),Yeast
  • 货号：
    CSB-YP885497DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana CBL-interacting serine/threonine-protein kinase 14(CIPK14),Yeast
  • 货号：
    CSB-EP885497DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana CBL-interacting serine/threonine-protein kinase 14(CIPK14),Yeast
  • 货号：
    CSB-EP885497DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana CBL-interacting serine/threonine-protein kinase 14(CIPK14),Yeast
  • 货号：
    CSB-BP885497DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana CBL-interacting serine/threonine-protein kinase 14(CIPK14),Yeast
  • 货号：
    CSB-MP885497DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CIPK14
  • Uniprot No.：
  • 别名：
    CIPK14; PKS24; SnRK3.15; SR1; At5g01820; T20L15.90CBL-interacting serine/threonine-protein kinase 14; EC 2.7.11.1; SNF1-related kinase 3.15; SOS2-like protein kinase PKS24; Serine/threonine-protein kinase SR1; AtSR1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-442
  • 氨基酸序列
    MVDSDPVEFP PENRRGQLFG KYEVGKLVGC GAFAKVYHGR STATGQSVAI KVVSKQRLQK GGLNGNIQRE IAIMHRLRHP SIVRLFEVLA TKSKIFFVME FAKGGELFAK VSKGRFCEDL SRRYFQQLIS AVGYCHSRGI FHRDLKPENL LLDEKLDLKI SDFGLSALTD QIRPDGLLHT LCGTPAYVAP EVLAKKGYDG AKIDIWSCGI ILFVLNAGYL PFNDHNLMVM YRKIYKGEFR IPKWTSPDLR RLLTRLLDTN PQTRITIEEI IHDPWFKQGY DDRMSKFHLE DSDMKLPADE TDSEMGARRM NAFDIISGSP GFNLSGLFGD ARKYDRVERF VSAWTAERVV ERLEEIVSAE NLTVAKKETW GMKIEGQKGN FAMVVEINQL TDELVMIEVR KRQRAAASGR DLWTDTLRPF FVELVHESDQ TDPEPTQVHT TS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner.
  • 基因功能参考文献：
    1. Study concludes that the CIPK14 phosphorylation-mediated WHY1 deficiency in plastids is related to the impairment of protein metabolism, leading to chloroplast dysfunction PMID: 30065159
    2. SR1 acts as transcription factor in balancing plant growth and immunity. PMID: 29899210
    3. results demonstrate that CIPK14 regulates the phosphorylation and organellar distributions of WHY1 and pinpoint that CIPK14 may function as a cellular switch between leaf senescence and plastid development for coordinating the intercellular signaling in Arabidopsis PMID: 28412544
    4. the calcium-independent activation of PKS24 by SCaBP1 is dependent on interaction of PKS24 with the C-terminal tail of SCaBP1.[PKS24] PMID: 24521877
    5. yeast two-hybrid assay demonstrated that CIPK14 interacted with three CBLs and also with two key kinases PMID: 25058457
    6. AtSR1 is an important component of plant resistance to insect herbivory as well as one of only three described proteins involved in Ca(2+)/Ccalmodulin (CaM)-dependent signaling to function in the regulation of glucosinolate (GS) metabolism. PMID: 23072934
    7. Results suggest that CIPK14 plays a role in PhyA-mediated FR inhibition of seedling greening, and that a Ca-related kinase may be involved in a previously undefined branch point in the phytochrome A signaling pathway. PMID: 21046322
    8. Induction of the gene by mono- and disaccharides and localization of expression to the leaf vasculature. PMID: 15665497
    9. Structural comparison of the two structures shows that the recognition of CBL by CIPK is performed in a similar manner, but inherent interactions confer binding affinity and specificity. PMID: 18237745
    10. Comparing with wild types, the lines inserted with the T-DNA in which CIPK14 gene expression was knocked out were also more sensitive to ABA and salt stimulus, showing low germination rate and the less root elongation PMID: 18785584

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Targeted to the tonoplast when interacting with CBL2 or CBL3 and to the cell membrane when interacting with CBL8.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
  • 组织特异性：
    Predominant in roots, cauline leaves, and flowers. Ubiquitous with highest expression in 7-day-old seedlings and flower buds, followed by that in cauline leaves and young siliques.
  • 数据库链接：

    KEGG: ath:AT5G01820

    STRING: 3702.AT5G01820.1

    UniGene: At.23738