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  4. Recombinant Arabidopsis thaliana Auxin transporter protein 1 (AUX1), partial

Recombinant Arabidopsis thaliana Auxin transporter protein 1 (AUX1), partial

  • 中文名称：
    Recombinant Arabidopsis thaliana Auxin transporter protein 1(AUX1),partial,Yeast
  • 货号：
    CSB-YP850215DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Auxin transporter protein 1(AUX1),partial,Yeast
  • 货号：
    CSB-EP850215DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Auxin transporter protein 1(AUX1),partial,Yeast
  • 货号：
    CSB-EP850215DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Auxin transporter protein 1(AUX1),partial,Yeast
  • 货号：
    CSB-BP850215DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Auxin transporter protein 1(AUX1),partial,Yeast
  • 货号：
    CSB-MP850215DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    AUX1
  • Uniprot No.：
  • 别名：
    AUX1; AUX; PIR1; WAV5; At2g38120; F16M14.5; Auxin transporter protein 1; Auxin influx carrier protein 1; Polar auxin transport inhibitor-resistant protein 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Carrier protein involved in proton-driven auxin influx. Mediates the formation of auxin gradient from developing leaves (site of auxin biosynthesis) to tips by contributing to the loading of auxin in vascular tissues and facilitating acropetal (base to tip) auxin transport within inner tissues of the root apex, and basipetal (tip to base) auxin transport within outer tissues of the root apex. Unloads auxin from the mature phloem to deliver the hormone to the root meristem via the protophloem cell files. Coordinated subcellular localization of AUX1 is regulated by a brefeldin A-sensitive (BFA) vesicle trafficking process. Involved in lateral root formation, trichoblast polarization and root hair elongation. Required for gravitropism and thigmotropism, especially in roots, by modulating responses to auxin, ethylene and cytokinins such as benzyladenine (BA). Needed for ammonium-mediated root-growth inhibition. Confers sensitivity to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D, auxin analog), and to polar auxin transport inhibitors such as N-1-naphthylphthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA).
  • 基因功能参考文献：
    1. AUX1-mediated root hair auxin influx governs SCF-type calcium signaling. PMID: 29563504
    2. RopGEF1 is critical for AUX1 localization. PMID: 28698357
    3. AUX1-mediated auxin transport rather than columella-located PIN proteins as a target of endogenous cytokinins in the control of root gravitropism. PMID: 27322763
    4. AUX1 and PIN2 protect lateral root (LR) formation in Arabidopsis during the early stages of iron (Fe) stress. PMID: 26468517
    5. Results suggest that Lateral Organ Boundaries Domain16 and 18 (LBD16 and LBD18) are important regulators of lateral root (LR) initiation and development downstream of AUXIN1 (AUX1) and LIKE-AUXIN3 (LAX3) auxin influx carriers. PMID: 26059335
    6. Data show that ethylene modulates alkaline stress-mediated inhibition of root growth by increasing auxin accumulation by stimulating the expression of auxin transporter protein 1 (AUX1) and auxin biosynthesis-related genes. PMID: 26109425
    7. Arabidopsis adventitious root formation and xylogenesis are developmental programmes that are inversely related, but they involve fine-tuning by the same proteins, namely SHR, SCR and AUX1. PMID: 25617411
    8. This study evaluated the role of AUX1 in the influx of auxins into cells. PMID: 25039492
    9. The aux1(rcr1) mutation may prevent DR5:GUS expression, possibly by affecting nuclear auxin signalling. PMID: 23293348
    10. The auxin influx gene AUX1 is directly associated with petal initiation, although detectable only when petal loss (PTL) function is lost. PMID: 23175631
    11. Shoot-supplied ammonium inhibits lateral root primordium emergence in Arabidopsis by interfering with AUX1-dependent auxin transport from shoot to root. PMID: 21342208
    12. Alkoxy-auxins are selective inhibitors of auxin transport mediated by PIN, ABCB, and AUX1 transporters. PMID: 21084292
    13. mutant analysis demonstrates that the auxin influx carrier LAX3 is indispensable for proper hook formation, whereas the auxin influx carrier AUX1 is involved in the hook exaggeration phenotype induced by ethylene PMID: 20110325
    14. Biochemical characterization of the auxin-influx carrier AUX1 is presented. PMID: 16677815
    15. results show that AXR4 (At1g54990) a protein of the endoplasmic reticulum (ER)regulates localization of AUX1; findings indicate that the axr4 agravitropic phenotype is caused by defective AUX1 trafficking in the root epidermis PMID: 16690816
    16. Planar polarity in root tips studied and found that combinatorial action of the auxin influx carrier AUX1, ETHYLENE-INSENSITIVE2, and GNOM genes mediates the vector for coordinate hair positioning. PMID: 17084699
    17. AUX1 uses a novel trafficking pathway in plants that is distinct from PIN trafficking, providing an additional mechanism for the fine regulation of auxin transport. PMID: 17114355
    18. auxin signaling in the basal meristem triggers pericycle cells for lateral root initiation prior to the action of INDOLE-3-ACETIC ACID PMID: 17215297
    19. for binding of IAA to AUX1 K(d) of 2.6 mum PMID: 18614710
    20. Mutations within the AUX1 gene reduces cytokinin regulated auxin accumulation and redistribution in calli and specific tissues of young seedlings. PMID: 19052775
    21. Three-dimensional modelling of auxin flow in the root tip suggests that AUX1-dependent transport through non-hair cells maintains an auxin supply to developing hair cells as they increase in distance from the root tip, and sustains root-hair outgrowth PMID: 19079245
    22. Data demonstrate that CTR1 acts as a concentration-dependent repressor of a biosynthesis-dependent auxin gradient that modulates planar polarity in the root tip. PMID: 19448626
    23. Data show that aux1 and pin2 mutants are insensitive to ABA-dependent repression of embryonic axis (hypocotyl and radicle) elongation. PMID: 19666738

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Note=In S2 columella cells, a dynamic cytoplasmic to membrane localization seems to occur during early stage of gravity signal transduction. In roots protophloem cells, asymmetric repartition in the upper plasma membrane.
  • 蛋白家族：
    Amino acid/polyamine transporter 2 family, Amino acid/auxin permease (AAAP) (TC 2.A.18.1) subfamily
  • 组织特异性：
    Expressed in root and shoot apical tissues. In root apex, confined to stele initials, protophloem poles, statolith-containing S2 columella cells, lateral root cap cells (LRC), and in epidermal cells from the distal elongation zone (DEZ) up to central elon
  • 数据库链接：

    KEGG: ath:AT2G38120

    STRING: 3702.AT2G38120.1

    UniGene: At.24295