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  4. Recombinant Arabidopsis thaliana Aquaporin PIP2-1 (PIP2-1), partial

Recombinant Arabidopsis thaliana Aquaporin PIP2-1 (PIP2-1), partial

  • 中文名称：
    拟南芥PIP2-1重组蛋白
  • 货号：
    CSB-YP337370DOA1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥PIP2-1重组蛋白
  • 货号：
    CSB-EP337370DOA1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥PIP2-1重组蛋白
  • 货号：
    CSB-EP337370DOA1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥PIP2-1重组蛋白
  • 货号：
    CSB-BP337370DOA1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥PIP2-1重组蛋白
  • 货号：
    CSB-MP337370DOA1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PIP2-1
  • Uniprot No.：
  • 别名：
    PIP2-1; PIP2A; At3g53420; F4P12.120; Aquaporin PIP2-1; Plasma membrane intrinsic protein 2-1; AtPIP2;1; Plasma membrane intrinsic protein 2a; PIP2a
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Water channel required to facilitate the transport of water across cell membrane. Probably involved in root water uptake. Its function is impaired by Hg(2+).
  • 基因功能参考文献：
    1. Phosphorylation of AtPIP2;1 Ser121 by BAK1 and/or OST1 is triggered in response to flg22 to activate its water and H2O2 transport activities. PMID: 28784763
    2. PIP2;1 activity is regulated by by Ca(2+) and pH. PMID: 27620834
    3. study reveals that C-terminal phosphorylation of a water channel subunit, PIP2;1, is upregulated by an EIN3EIL1-independent ethylene signaling pathway and its water transport activity is enhanced by phosphorylation at the C-terminal S280 and S283 sites PMID: 26476206
    4. This work supports a model whereby abscissic acid-triggered stomatal closure requires an increase in guard cell permeability to water and possibly hydrogen peroxide, through OST1-dependent phosphorylation of PIP2;1 at Ser-121. PMID: 26163575
    5. PIP2;1 molecules are heterogeneously distributed on the plasma membrane and that clathrin and membrane raft pathways cooperate to mediate the subcellular trafficking of PIP2;1. PMID: 22010034
    6. Co-expression of AtPIP2;1-GFP and AtPIP1;4-mCherry constructs suggested that ER-retained AtPIP2;1-GFP may interact with other PIPs to hamper their trafficking to the PM, thereby contributing to inhibition of root cell hydraulic conductivity PMID: 21182578
    7. analysis of the subcellular trafficking of AtPIP2 PMID: 18234664
    8. Structure-function analysis of plant aquaporin AtPIP2. PMID: 18637793

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Note=A fuzzy intracellular localization is induced by salt (NaCl) treatment.
  • 蛋白家族：
    MIP/aquaporin (TC 1.A.8) family, PIP (TC 1.A.8.11) subfamily
  • 组织特异性：
    Predominantly expressed in roots and green siliques. Also expressed at lower level above ground and in flower buds.
  • 数据库链接：

    KEGG: ath:AT3G53420

    STRING: 3702.AT3G53420.1

    UniGene: At.47609