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  4. Recombinant Apoptosis regulator ced-9 (ced-9)

Recombinant Apoptosis regulator ced-9 (ced-9)

  • 货号：
    CSB-YP336943CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP336943CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP336943CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP336943CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP336943CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ced-9
  • Uniprot No.：
  • 别名：
    ced-9; T07C4.8Apoptosis regulator ced-9; Cell death protein 9
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-280
  • 氨基酸序列
    MTRCTADNSL TNPAYRRRTM ATGEMKEFLG IKGTEPTDFG INSDAQDLPS PSRQASTRRM SIGESIDGKI NDWEEPRLDI EGFVVDYFTH RIRQNGMEWF GAPGLPCGVQ PEHEMMRVMG TIFEKKHAEN FETFCEQLLA VPRISFSLYQ DVVRTVGNAQ TDQCPMSYGR LIGLISFGGF VAAKMMESVE LQGQVRNLFV YTSLFIKTRI RNNWKEHNRS WDDFMTLGKQ MKEDYERAEA EKVGRRKQNR RWSMIGAGVT AGAIGIVGVV VCGRMMFSLK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a major role in programmed cell death (PCD, apoptosis). egl-1 binds to and directly inhibits the activity of ced-9, releasing the cell death activator ced-4 from a ced-9/ced-4 containing protein complex and allowing ced-4 to activate the cell-killing caspase ced-3. During larval development, required for the elimination of transient presynaptic components downstream of egl-1 and upstream of ced-4 and ced-3 apoptotic pathway.
  • 基因功能参考文献：
    1. HBx interacts directly with the B-cell lymphoma 2 (Bcl-2) homolog CED-9 (cell death abnormal) through a Bcl-2 homology 3 (BH3)-like motif to trigger both cytosolic Ca(2+) increase and cell death. PMID: 23091037
    2. propose that EGL-1 converts CED-9 into a mitochondrial receptor for DRP-1, thereby shifting its activity from profusion to profission. PMID: 21949250
    3. CED-9 retains the amphitropic ability of mammalian Bcl-2 proteins to associate with cellular membranes. PMID: 21031486
    4. CED-9 forms a 2 : 2 heterotetrameric complex with CED-4 until specifically displaced by Caenorhabditis elegans, but not mammalian, BH3-only proteins. PMID: 16167070
    5. Here, we show that CED-9 interacts with Mitofusin-2/fuzzy onions and can promote mitochondrial clustering and dramatic reorganization of mitochondrial networks. PMID: 16543146
    6. these data are consistent with a multimodal character of CED-9 action, with an ability to regulate apoptosis through interactions in the cytosol coexisting with additional evolutionarily conserved role(s) at the membrane PMID: 17703231
    7. while bcl-2 proteins are not required for ceramide to form protein-permeable channels in mitochondrial outer membranes, both recombinant human Bcl-x(L) and CED-9 disassemble ceramide channels in the mitochondrial outer membranes from rat liver and yeast PMID: 18171672
    8. CED-9 is capable of regulating the mitochondrial fission-fusion cycle but is not essential for either fission or fusion. PMID: 18827010
    9. Ced-9 promotes Al tolerance in plants via inhibition of Al-induced programmed cell death. PMID: 19341713

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  • 亚细胞定位：
    Perikaryon. Cell junction, synapse. Endomembrane system; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein.
  • 蛋白家族：
    Bcl-2 family
  • 数据库链接：

    KEGG: cel:CELE_T07C4.8

    STRING: 6239.T07C4.8

    UniGene: Cel.6305