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  4. SLC16A1 Recombinant Monoclonal Antibody

SLC16A1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA919798A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, K562 whole cell lysate
      All lanes: MCT1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 54, 47 kDa
      Observed band size: 54 kDa
    • IHC image of CSB-RA919798A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA919798A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    The proton-linked monocarboxylate transporter 1 (MCT1), encoded by the SLC16A1 gene, is a proton-dependent cotransporter/exchanger found on the apical membrane of cells. Many monocarboxylates, such as lactate and pyruvate, branched-chain oxoacids produced from leucine, valine, and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate, and acetate, are transported across the cell membrane via MCT1. In an animal model, MCT1 mutation or reduced expression causes neuronal death due to axonal degeneration. Patients with recurrent ketoacidosis and hypoglycemia, a potentially fatal illness, were found to have inactivating recessive homozygous or heterozygous mutations in the SLC16A1 gene.

    The first step in the preparation of recombinant SLC16A1 antibody is to obtain the SLC16A1 antibody gene. The heavy and light chain genes of the antibody were constructed into a plasma vector and then transfected into suspended mammalian cells transiently. After expression verification, cell supernatant was collected in expanded culture and purified recombinant SLC16A1 antibody was obtained using Affinity-chromatography. This recombinant SLC16A1 antibody has been validated for the detection of SLC16A1 protein from Human in the ELISA, WB, IHC.

  • Uniprot No.：
    P53985
  • 基因名：
    SLC16A1
  • 别名：
    Monocarboxylate transporter 1 (MCT 1) (Solute carrier family 16 member 1), SLC16A1, MCT1
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Monocarboxylic acid transporter 1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    9D12
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.
  • 基因功能参考文献：
    1. 7ACC2 is an inhibitor of mitochondrial pyruvate transport; the blockade of pyruvate import into mitochondria prevents extracellular lactate uptake as efficiently as a MCT1 inhibitor PMID: 29572438
    2. the MCT1 A allele is associated with forward soccer player status PMID: 30290372
    3. High MCT-1 expression is associated with clear cell renal cell carcinoma. PMID: 29657088
    4. Metabolism-dependent clonal growth of HCT15 colorectal cancer cells was induced by Nrf2-dependent activation of MCT1-driven lactate exchange. PMID: 28846107
    5. These results demonstrate that Monocarboxylate transporters tend to play a role in the aggressive breast cancer subtypes through the dynamic interaction between breast cancer cells and adipocytes. PMID: 29775610
    6. Sub-Saharan African groups show extremely high values of the T allele of 1470T > A polymorphism. The TT genotype preeminence in African groups could explain the better predisposition to sprint/power performances of African athletes. Caucasian and Asian populations show variable proportions of TT and AA genotypes allowing inter-individual differences in lactate transport. PMID: 29985759
    7. Using in vitro models, we demonstrate that tumor-excreted branched-chain amino acid (BCKA)s can be taken up and re-aminated to BCAAs by tumor-associated macrophages. Our data further suggest that the anti-proliferative effects of MCT1 knockdown observed by others might be related to the blocked excretion of BCKAs. PMID: 29066459
    8. Hypoxia-induced MCT1 supports glioblastoma glycolytic phenotype, being responsible for lactate efflux and an important mediator of cell survival and aggressiveness PMID: 27331625
    9. our finding that the expression of MCT1 and MCT4 is reduced in mutant IDH1 gliomas highlights the unusual metabolic reprogramming that occurs in mutant IDH1 tumors and has important implications for our understanding of these tumors and their treatment PMID: 27144334
    10. MCT1 expression, independent of transporter activity, is required for growth factor-induced tumor cell motility. PMID: 27127175
    11. TOMM20, MCT1, and MCT4 expression was significantly different in Hodgkin and Reed Sternberg (HRS) cells. HRS have high expression of MCT1, while tumor associated macrophages have absent MCT1 expression. Tumor-infiltrating lymphocytes have absent MCT1 expression. Reactive lymph nodes in contrast to cHL tumors had low TOMM20, MCT1, and MCT4 expression in lymphocytes and macrophages. PMID: 29248133
    12. TOMM20 and MCT1 were highly expressed in diffuse large B-cell lymphoma lymphocytes, indicating an OXPHOS phenotype, whereas non-neoplastic lymphocytes in the control samples did not express these markers. PMID: 29248132
    13. Data suggest that targeting monocarboxylate transporter 1 (MCT1) in both tumor cells and brain endothelial cells (EC) may be a promising therapeutic strategy for the treatment of Glioblastoma (GBM). PMID: 28762551
    14. The structures and functions of hMCT1 and hMCT4 transporters. PMID: 28559188
    15. MCT1 inhibitor AZD3965 increased MCT4-dependent accumulation of intracellular lactate, inhibiting monocarboxylate influx and efflux. PMID: 28923861
    16. Silencing or genetic deletion of MCT1 in vivo inhibited migration, invasion, and spontaneous metastasis. PMID: 28827372
    17. The reversible H(+)/lactate(-) symporter MCT1 cotransports lactate and proton, leading to the net extrusion of lactic acid in glycolytic tumors. A model of its role in pH control in tumor cells is described. Review. PMID: 26944480
    18. Monocarboxylate transporters (MCTs) are expressed in normal and cancer cells and are involved in cell metabolism and survival. PMID: 27957817
    19. Increased miR-210 and concomitant decreased ISCU RNA levels were found in ~40% of tumors and this was significantly associated with HIF-1alpha and CAIX, but not MCT1 or MCT4, over-expression. PMID: 28099149
    20. Data show that metastasis-associated in colon cancer-1 (MACC1) and monocarboxylate transporter 1 (MCT1) are highly expressed in gastric cancer indicating poor prognosis. PMID: 28235486
    21. AA genotype of the MCT1 T1470A polymorphism is over-represented in wrestlers compared with controls and is associated with lower blood lactate concentrations after 30-s Wingate Anaerobic test and during intermittent sprint tests in Japanese wrestlers PMID: 27813046
    22. MCT1 and MCT4 expression levels were associated with worse prognosis and shorter overall survival. PMID: 27105345
    23. MCT1 may be acting as an uptake transporter and MCT4 as an efflux system across the basolateral membrane for ferulic acid, and that this process is stimulated by butyric acid. PMID: 26854723
    24. Data suggest that inhibition of mnocarboxylate transporters MCT1 and MCT4 may have clinical relevance in pancreatic ductal adenocarcinoma (PDAC). PMID: 26765963
    25. Results found that MCT contributes to endothelial cell growth and tube formation via up-regulation of angiopoietin-1 expression suggesting that MCT plays an important role in pancreatic cancer angiogenesis and tumor growth via activating the angiopoietin-1 pathway. PMID: 27240355
    26. The targeting of MCT1 and PFKFB3 regulated cell proliferation. PMID: 27373212
    27. MCT1 expression associates with the SCC type and metastatic behavior of AC, whereas MCT4 expression concomitantly increases from in situ SCC to invasive SCC and is significantly associated with the AC type. Consistently, FOXM1 expression is statistically associated with MCT1 positivity in SCC, whereas the expression of FOXO3a, a FOXM1 functional antagonist, is linked to MCT1 negativity PMID: 26563366
    28. This study investigated MCT1 protein abundance in various human intestinal tissues. PMID: 27634412
    29. MCT1 affects the plasma lactate decrease . PMID: 27026015
    30. The aim of this study was to investigate the association between the MCT1 A1470T polymorphism and fat-free mass in young Italian elite soccer players. The MCT1 T allele is associated with the percentage of fat-free mass in young elite male soccer players. PMID: 26340466
    31. MCT1 inhibition impairs proliferation of glycolytic breast cancer cells co-expressing MCT1 and MCT4 via disruption of pyruvate rather than lactate export. PMID: 26876179
    32. MCT1 expression was not clearly associated with overall or disease-free survival. MCT4 and CD147 expression correlate with worse prognosis across many cancer types. These results warrant further investigation of these associations. PMID: 26779534
    33. The most important finding from the present study was that wild-type allele carriers, 2917(1414)T and IVS3-17C, have been associated with higher blood lactate levels at submaximal intensities of exercise for the first time. PMID: 26595136
    34. MCT1 is highly expressed in anaplastic thyroid cancer compared to non-cancerous thyroid tissue and papillary thyroid cancer. PMID: 26615136
    35. interaction of rmEMMPRINex with U937 cells leads to inhibition of MCT1 membrane expression, intracellular activation of procaspase-9, followed by DNA fragmentation and apoptosis. PMID: 26024713
    36. Down regulation of MCT1 promote the sensitivity to cisplatin in ovarian cancer cells. This effect appeared to be mediated by antagonizing Fas. PMID: 26045776
    37. T-allele frequency was significantly higher among swimmers compared with runners. PMID: 24708341
    38. The findings indicated that theMCT1 TT genotype was overrepresented in sprint/power athletes compared to both endurance athletes and non-athlete controls. PMID: 24485392
    39. The possible cooperative role of CD147 and MCT1 in determining cisplatin resistance. PMID: 25263481
    40. The first IF method has been developed and optimised for detection of MCT 1 and MCT4 in cancer patient circulating tumour cells PMID: 25957999
    41. CD147 interacts with MCT1 to regulate tumor cell glycolysis, resulting in the progression of thyroid carcinoma. PMID: 25755717
    42. MCT1 was up-regulated by exposure to DL-2-hydroxy-(4-methylthio)butanoic acid(HMTBA). Moreover, total monolayer MCT1 immunoreactivity increased 1.8-fold in HMTBA-supplemented cultures, this effect mainly being localised at the apical membrane. PMID: 25447800
    43. Our findings suggest that SNPs in MCT1 and MCT2 genes may affect clinical outcomes and can be used to predict the response to adjuvant chemotherapy in NSCLC patients who received surgical treatment once validated in future study. PMID: 25578492
    44. SNPs in MCT1 and MCT2 genes may affect clinical outcomes and can be used to predict the response to adjuvant chemotherapy in CRC patients who received surgical treatment PMID: 25492048
    45. These results indicate there are no additional benefits of IHT when compared to similar normoxic training. Hence, the addition of the hypoxic stimulus on anaerobic performance or MCT expression after a three-week training period is ineffective. PMID: 24797797
    46. We provide novel information of MCT1 as a candidate marker for prognostic stratification in NSCLC. PMID: 25225794
    47. H(+)-coupled 5-oxoproline transport is mediated solely by SLC16A1 in astrocytes PMID: 25371203
    48. Data suggest that expression of MCT1 in intestinal mucosa can be altered by diet; here, expression of MCT1 is down-regulated in Caco-2 Cells by products of fermentation of dietary proteins by intestinal microbes. PMID: 25656974
    49. Overexpression of MCT1, MCT4, and CD147 predicts tumor progression in clear cell renal cell carcinoma. PMID: 25456395
    50. Data indicate that pteridine showed substantial anticancer activity in monocarboxylate transporter 1 (MCT1)-expressing Raji lymphoma cells. PMID: 25068893

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  • 相关疾病：
    Symptomatic deficiency in lactate transport (SDLT); Familial hyperinsulinemic hypoglycemia 7 (HHF7); Monocarboxylate transporter 1 deficiency (MCT1D)
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Major facilitator superfamily, Monocarboxylate porter (TC 2.A.1.13) family
  • 组织特异性：
    Detected in heart and in blood lymphocytes and monocytes (at protein level). Widely expressed.
  • 数据库链接：

    HGNC: 10922

    OMIM: 245340

    KEGG: hsa:6566

    STRING: 9606.ENSP00000358640

    UniGene: Hs.75231