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  4. RPTOR Recombinant Monoclonal Antibody

RPTOR Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA581950A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA581950A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA581950A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    RPTOR, also called RAPTOR, an essential component of mTORC1, functions as a scaffold for mTOR kinase to recruit and phosphorylate specific substrates such as S6K1 and to initiate the nucleic acid synthesis and cap-dependent translation as well as SREBP1 to promote cell metabolism. It is essential for mTORC1 activity and critical for the regulation of mTORC1 activity in response to insulin signaling and nutrient and energy sufficiency. RAPTOR also plays an instructive role in the development and function of B cells, and the deficiency of RAPTOR impairs the survival and proliferation of pre-B cells.

    The recombinant RPTOR antibody was produced by cloning antibody genes into an expression vectors, which were subsequently introduced into mammalian cells to provide animal-free antibody production. This RPTOR antibody has been validated in ELISA, IHC. It has the features of improved affinity, stability, and consistency between different batches.

  • Uniprot No.：
    Q8N122
  • 基因名：
  • 别名：
    Regulatory-associated protein of mTOR (Raptor) (p150 target of rapamycin (TOR)-scaffold protein), RPTOR, KIAA1303 RAPTOR
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Raptor
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    1B10
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis. mTORC1 complex in excitatory neuronal transmission is required for the prosocial behavior induced by the psychoactive substance lysergic acid diethylamide (LSD).
  • 基因功能参考文献：
    1. Study shows that USP9X deubiquitylating enzyme maintains RAPTOR protein levels, mTORC1 signalling and proliferation in neural progenitors. USP9X is the first deubiquitylating enzyme shown to stabilize RAPTOR. PMID: 28341829
    2. Results suggest that RPTOR mediates, at least partially, the resistance to EGFR inhibition in triple-negative breast cancer cells. PMID: 29344641
    3. data reveal that RAPTOR up-regulation contributes to PI3K-mTOR inhibitor resistance, and suggest that RAPTOR expression should be included in the pharmacodynamic assessment of mTOR kinase inhibitor trials PMID: 29389967
    4. Hypomethylation of CpG sites in RPTOR, MGRN1 and RAPSN in blood is associated with breast cancer. PMID: 27577081
    5. RPTOR (regulatory associated protein of mTOR, complex 1) is a novel target of miR-155 in CF lung epithelial cells. The suppression of RPTOR expression and subsequent activation of TGF-beta signaling resulted in the induction of fibrosis by elevating connective tissue growth factor (CTGF) abundance in CF lung epithelial cells. PMID: 27284727
    6. Data show that mTOR protein forms a complex with Raptor and estrogen receptor-alpha (ERalpha). PMID: 26522726
    7. Up-regulation of mTORC1 via raptor by aldosterone is a critical pathobiologic mechanism that controls pulmonary artery smooth muscle cell survival to promote hypertrophic vascular remodeling and pulmonary arterial hypertension. PMID: 27006450
    8. Hgh mTOR activity and Rictor overexpression could be markers of a bad prognosis. Combined phosphoprotein and Rictor/Raptor expression evaluation revealed even stronger statistical correlation with prognosis. PMID: 27729429
    9. revealed more than 170 NFAT-associated proteins, half of which are involved in transcriptional regulation. Among them are many hitherto unknown interaction partners of NFATc1 and NFATc2 in T cells, such as Raptor, CHEK1, CREB1, RUNX1, SATB1, Ikaros, and Helios. PMID: 27637333
    10. In this study we began by validating the expression of four main mTOR pathway components, mTOR, DEPTOR, rictor and raptor, at gene and protein level in in vitro models of endometrioid (MDAH2774) and clear cell (SKOV3) ovarian cancer PMID: 27211906
    11. Our results demonstrate for the first time that the expression quantitative trait loci of RPTOR, rs7502563, is susceptible to glioma PMID: 26361958
    12. The positive regulation of mTORC1 activity by NPRL2 is mediated through NPRL2 interaction with Raptor. PMID: 26582740
    13. RPTOR was not associated with bipolar disorder or schizophrenia. PMID: 25053281
    14. microRNA-210 and raptor are involved in mithramycin-mediated erythroid differentiation of K562 cells and participate to the fine-tuning and control of gamma-globin gene expression in erythroid precursor cells. PMID: 25849663
    15. the expressions of Beclin1, Raptor, and Rictor are related to the development and progression of colorectal carcinoma and multidrug resistance. PMID: 26363527
    16. Common genetic variation in RPTOR is associated with overweight/obesity but does not discernibly contribute to either essential hypertension or isolated systolic hypertension in the Hawaiian population studied. PMID: 25249372
    17. Studied the impact of SNPs in/near UCP3 and RPTOR on obesity-related traits. PMID: 25533680
    18. phosphorylating the T-loop Akt residue Thr(308) by PDK1 requires Raptor of the mTORC1 complex as a platform or scaffold protein. PMID: 24516643
    19. suggest no connection of RPTOR variants with psoriasis or its subphenotypes. PMID: 24005976
    20. peptides that encompass the Raptor cross-linking region of 4E-BP1 inhibit cross-linking and interaction of 4E-BP1 with Raptor. PMID: 24403073
    21. Amino acids promote mTORC1 activation without altering Rag GTP charging. PMID: 24337580
    22. There is a significant downregulation of mTOR, DEPTOR, and Raptor in preterm labouring myometria when compared to non-pregnant tissues taken from the same area. PMID: 23541542
    23. Raptor polymorphism is associated with gastric cancer. PMID: 23423739
    24. Histolopathological and clinical information including tumour stage, invasion characteristic and endocrine status were analysed against the gene transcript expression of mTOR, RAPTOR and RICTOR. PMID: 23898069
    25. Single nucleotide polymorphisms in RPTOR gene is associated with obesity. PMID: 23563607
    26. An essential role of mTORC1 in autophagy inhibition in cell-free system in which, membrane association of Barkor/Atg14(L), a specific autophagosome-binding protein, is suppressed by cytosol from nutrient-rich medium, is shown. PMID: 22258093
    27. show that E2F1 is capable of inducing growth by regulating mTORC1 activity PMID: 21283628
    28. Adiponectin induces vascular smooth muscle cell differentiation via repression of mammalian target of rapamycin complex 1 and FoxO4 PMID: 21454807
    29. results suggest that GRp58/ERp57 is involved in the assembly of mTORC1 and positively regulates mTORC1 signaling at the cytosol and the cytosolic side of the endoplasmic reticulum PMID: 21321085
    30. found that osmotic stress activates mTORC1 kinase activity in a JNK-dependent manner. Our findings suggest that the molecular link between JNK and Raptor is a potential mechanism by which stress regulates the mTORC1 signaling pathway PMID: 22493283
    31. an important role for ICK in modulating the activity of mTORC1 through phosphorylation of Raptor Thr-908. PMID: 22356909
    32. Phosphorylated forms of Raptor are significantly increased in mitotic cells. Raptor resides at the nucleoplasm and nucleolus of interphase cells and remains targeted to nucleolar organizer regions during mitosis. PMID: 21900751
    33. ULK1 contributes to mTORC1 inhibition through hindrance of substrate docking to Raptor. PMID: 21460630
    34. a previously unrecognized function of miR-21 that is the reciprocal regulation of PTEN levels and Akt/TORC1 activity that mediate critical pathologic features of diabetic kidney disease PMID: 21613227
    35. The study identified raptor as a substrate for p44/42 MAPK. PMID: 21325048
    36. Data show that a SNP (rs11868112) 26 kb upstream to the transcription start site of RPTOR exhibiting the strongest association with temperature variables. PMID: 21060808
    37. a novel regulatory mechanism by which mitogenic and oncogenic activation of the Ras/MAPK pathway promotes mTOR signaling PMID: 21071439
    38. there are two parallel cell-survival pathways in prostate cancer cells: a strong Akt-independent, but rapamycin-sensitive pathway downstream of mTORC1, and an AR-dependent pathway downstream of mTORC2 and Akt, that is stimulated by mTORC1 inhibition PMID: 18776922
    39. Study demonstrates that Serine 696 and Threonine 706 represent two key sites in raptor phosphorylation during mitosis, and that the mitotic cyclin-dependent kinase cdc2/CDK1 is the kinase responsible for phosphorylating these sites. PMID: 20169205
    40. These data suggest that the raptor/mTORC1 pathway may play a role in increased IRES-dependent mRNA translation during mitosis and in rapamycin insensitivity. PMID: 20439490
    41. S2448 phosphorylated mTOR binds to both raptor and rictor. PMID: 19145465
    42. Complex raptor phosphorylation functions as a biochemical rheostat that modulates mTORC1 signaling in accordance with environmental cues. PMID: 19864431
    43. Raptor is a missing component of the mTOR pathway that through its association with mTOR regulates cell size in response to nutrient levels PMID: 12150925
    44. raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo. PMID: 12150926
    45. raptor binds to p70S6k and 4E-BP1 through their respective TOS (conserved TOR signaling) motifs. PMID: 12604610
    46. A redox-sensitive mechanism regulates the phosphorylation of the raptor-mTOR effector S6K1, the interaction between raptor and mTOR, and the kinase activity of the raptor-mTOR complex. PMID: 16183647
    47. Raptor directly binds to and serves as a scaffold for mTOR-mediated phosphorylation of IRS-1 on Ser636/639. PMID: 16354680
    48. These studies suggest that, through serine phosphorylation, Raptor-mTOR and S6K1 promote the depletion of IRS1 from specific intracellular pools in pathological states of insulin and IGF-I resistance and in lesions associated with tuberous sclerosis. PMID: 16914728
    49. These data suggest that, during HCMV infection, the rictor- and raptor-containing complexes are modified such that their substrate specificities and rapamycin sensitivities are altered. PMID: 16959881
    50. ASCT2 silencing inhibits mTORC1 (mTOR/raptor) signaling and leads to growth repression, followed by enhanced survival signaling via mTORC2 (mTOR/rictor) and apoptosis of hepatoma cells. PMID: 17329400

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  • 亚细胞定位：
    Cytoplasm. Lysosome. Cytoplasmic granule. Note=Targeting to lysosomes depends on amino acid availability. In arsenite-stressed cells, accumulates in stress granules when associated with SPAG5 and association with lysosomes is drastically decreased.
  • 蛋白家族：
    WD repeat RAPTOR family
  • 组织特异性：
    Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3 is widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen.
  • 数据库链接：

    HGNC: 30287

    OMIM: 607130

    KEGG: hsa:57521

    STRING: 9606.ENSP00000307272

    UniGene: Hs.133044