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  4. LGR5 Recombinant Monoclonal Antibody

LGR5 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA262034A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue
      All lanes: LGR5 antibody at 1:1500
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 100, 98, 93 kDa
      Observed band size: 100 kDa
    • Overlay histogram showing HepG2 cells stained with CSB-RA262034A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：

    The first step in the preparation of recombinant LGR5 antibody is to obtain the LGR5 antibody gene. The heavy and light chain genes of the antibody were constructed into a plasma vector and then transfected into suspended mammalian cells transiently. After expression verification, cell supernatant was collected in expanded culture and purified recombinant LGR5 antibody was obtained using affinity-chromatography. This recombinant LGR5 antibody has been validated for the detection of LGR5 protein from Human, Rat in the ELISA, WB, FC.

    LGR5, also known as GPR49, is a stem cell marker found in the crypts of the intestine and the mammary glands. The protein LGR5 is required for proper embryonic development. In the presence of the ligand R-spondin, LGR5 controls Wnt signaling (RSPO). LGR5 stimulates cancer stem cell proliferation and self-renewal by potentiating the Wnt/β-catenin signaling pathway. LGR5 has been shown to increase cancer cell mobility, tumor formation, and epithelial-mesenchymal transition in breast cancer cells by activating Wnt/β-catenin signaling. It has also been reported that high LGR5 expression is positively related to shorter patient survival.

  • Uniprot No.：
    O75473
  • 基因名：
  • 别名：
    Leucine-rich repeat-containing G-protein coupled receptor 5 (G-protein coupled receptor 49) (G-protein coupled receptor 67) (G-protein coupled receptor HG38), LGR5, GPR49 GPR67
  • 反应种属：
    Human, Rat
  • 免疫原：
    A synthesized peptide derived from human LGR5/GPR49
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    4H8
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    FC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
  • 基因功能参考文献：
    1. Data conclude that LGR5 expression in CTCs may serve as a marker for CRC metastasis. PMID: 29949050
    2. LGR5 is a new functional GSC marker and prognostic indicator that can promote EMT by activating the Wnt/beta-catenin pathway and would thus be a novel therapeutic target for glioma. PMID: 30208924
    3. Data found LGR5 as a potential target of miR-340. LGR5 was highly expressed in breast cancer cells. Relative expression of LGR5 was negatively regulated by miR-340. Knockdown of LGR5 also inhibited cell proliferation and drug resistance to docetaxel with enhanced cell apoptosis of breast cancer cells. PMID: 30300682
    4. knockdown of CASC15 significantly inhibited the proliferation, migration and invasion of colon cancer cells in vitro and in vivo. Following mechanistic experiments, CASC15 was observed to act as a sponge to suppress microRNA (miR)4310 that targeted LGR5. PMID: 29956772
    5. LGR5 overexpression was associated with high T stage and LN metastasis status in breast cancer. High LGR5 expression was also associated with reduced relapse-free survival, indicating that LGR5 may represent a promising prognostic marker for breast cancer patients. PMID: 29471794
    6. LGR5 is a critical effector of DDX1 in colorectal cancer cells. PMID: 29869821
    7. Logarithmic expansion of LGR5-expressing cells was observed in human colorectal cancer. PMID: 28958617
    8. Our findings indicated that the Lgr5High/DCLK1High expression pattern may be considered as a signature phenotype for intestinal subtypes of gastric carcinoma. PMID: 28946555
    9. Findings suggest a tumor suppressor role for miR-216a in gliomas, which inhibits glioma cell proliferation, migration, and invasion by targeting LGR5. PMID: 28256193
    10. LGR5 promotes cancer stem cell traits and chemoresistance in cervical cancer. PMID: 28880275
    11. LGR5 expression in the epithelium and stroma was associated with tumor stage, and by integrating functional experiments with LGR5-sorted cell RNA sequencing data from adenoma and normal organoids, there were correlations between LGR5 and CRC-specific genes, including dickkopf WNT signaling pathway inhibitor 4 (DKK4) and SPARC-related modular calcium binding 2 (SMOC2 PMID: 29467240
    12. LGR5 expression is transiently upregulated during the early stage of cardiomyocyte differentiation from hPSCs, and although LGR5 expression is not required for maintaining hPSCs in the undifferentiated state, knockdown of LGR5 leads to decreased expression of key cardiac transcription factors at the early stage with eventual lack of robust beating cardiomyocytes at the late stage. PMID: 28793247
    13. Lgr5 plays important roles in angiogenesis. Lgr5-specific siRNA could be designed into an effective therapeutic agent to inhibit gastric cancer angiogenesis. PMID: 28404940
    14. A regulatory mechanism of LGR5 expression in gastric carcinogenesis, with SP1 as an important component of the transcriptional complex and LGR5 activity, which is modulated by its ligands RSPO1 and RSPO2, whose expression is modulated by methylation. PMID: 28219935
    15. these results demonstrate that Lgr5 activation of Wnt/beta-catenin signaling is a potential mechanism to promote the progression of ESCC and ESCC stem cell renewal, and Lgr5 may be used as a molecular target for the development of treatments for ESCC. PMID: 28404917
    16. This study uncovers a novel cross-talk between LGR5 and TGFbeta signaling in colon cancer and identifies LGR5 as a new modulator of TGFbeta signaling able to suppress colon cancer metastasis. PMID: 28939678
    17. Data show that IKKalpha directly bind to the promoters of LGR5, in turn, upregulating LGR5 expression through activation of STAT3 signaling pathway during cancer progression. PMID: 27049829
    18. Report the presence of aberrantly located LGR5(+) cells coexpressing epithelial markers in the stromal compartment of women with endometriosis. These cells have a statistically significantly different expression profile in deep-infiltrating endometriosis in comparison with other types of endometriosis, independent of the menstrual cycle phase. PMID: 28923287
    19. Arrb1 reduced the chemotherapy-induced Lgr5 stem cell apoptosis by inhibiting endoplasmic reticulum stress-mediated mitochondrial apoptotic signaling. PMID: 27195676
    20. LGR5 signaling in gastric cancer cells.LGR5 crosstalk with FOXO1 in gastric cancer cells. PMID: 28970066
    21. High LGR5 expression is associated with osteoarthritis. PMID: 28777797
    22. The LGR5 primarily functions via the IQGAP1-Rac1 pathway to strengthen cell-cell adhesion in normal adult crypt stem cells and colon cancer cells. PMID: 28739799
    23. Lgr5(+) chief cells are involved in maintaining the homeostatic stem cell pool. Define Lgr5(+) chief cells as a major cell-of-origin of gastric cancer. PMID: 28581476
    24. LGR5-expressing fraction of CD54+ cells represents gastric cancer CSCs, in which LGR5 is closely associated with stemness and EMT core genes PMID: 28033430
    25. clathrin-mediated endocytosis inhibition of the leucine-rich G protein-coupled receptor-5 diminishes cell fitness PMID: 28275053
    26. The findings indicated that Lgr5 might contribute to the intestinal metaplasia during gastric carcinogenesis. PMID: 26077638
    27. Endometrial LGR5 is not an endogenous stem cell marker. Instead, LGR5(+) cells appear to be recruited from blood to be part of the stem cell niche at the perivascular microenvironment to activate the endogenous niche. PMID: 27887719
    28. 2 LGR5 splice variants were expressed in human intestine crypt cells; one lacked exon 5 and the other lacked exons 5-8. Only LGR5FL appeared during cell cycle arrest. The transcript variants appeared when the cell cycle was proceeding. LGR5FL-positive cells were negative for Ki-67 and were enriched after chemotherapy. PMID: 27140312
    29. Low expression of LGR5 is associated with gastric cancer. PMID: 26386561
    30. Report targeting LGR5+ tumor stem cells with an antibody-drug conjugate as effective treatment for colon cancer. PMID: 26582901
    31. Lgr5 overexpression was significantly associated with deep invasion of colorectal cancer lymphnode metastasis, distant metastasis and AJCC stage PMID: 26758198
    32. Data suggest that targeting of leucine rich repeat containing G protein-coupled receptor 5 (LGR5) may be of therapeutic benefit for neuroblastomas. PMID: 26517508
    33. This study shows that Lgr5 can be a valuable and reliable prognostic factor of colorectal cancer progression. PMID: 26674601
    34. We conclude that overexpression of LGR5 is associated with markers of tumor aggressiveness in human papillary thyroid cancer PMID: 26416247
    35. The results suggest that trichosanthin may induce apoptosis in glioma cells by targeting LGR5 and repressing the Wnt/betabcatenin signaling pathway PMID: 26397053
    36. These findings indicate that LGR5 not only participates in carcinogenesis but also maintained stemness by activating Wnt/b-catenin signaling in breast cancer. PMID: 26086949
    37. We confirmed in a large and independent study cohort that LGR5 rs17109924 is a predictive genetic biomarker for time to recurrence in patients with colon cancer treated with 5-FU-based adjuvant chemotherapy. PMID: 25665511
    38. lgr5 methylation, through the regulation of lgr5 expression and colorectal CSC differentiation, may constitute a novel prognostic marker for colorectal cancer patients. PMID: 26599100
    39. High resolution crystal structure of an ectodomain variant of human LGR5 (hLGR5ecto) complexed with a signalling competent fragment of mouse Rspo2. PMID: 26123262
    40. Lgr5-positive cells may be cancer stem cells-like cells in gastric cancer. PMID: 26279446
    41. Demonstrate the presence of LGR5-positive cells in limbal epithelial crypts and their decrease in inflamed conditions, which suggests a critical role of this protein during inflammation and its possible use as a marker in normal crypts. PMID: 26097379
    42. Elevated Lgr5 expression might contribute to the development and progression of colorectal cancer. PMID: 24751002
    43. data reveal a RSPO2-induced, LGR5-dependent Wnt signaling-negative feedback loop that exerts a net growth-suppressive effect on CRC cells PMID: 24476626
    44. our data highlight a potential role of Lgr5-positive cells in the tumorigenesis of colorectal cancer (CRC), and suggest that treating these Lgr5-positive cells in CRCs may substantially improve the outcome of CRC therapy PMID: 25835970
    45. Lgr5 may play an important role in the development and progression of cervical carcinoma. PMID: 25973068
    46. Based on the lack of correlation between Lgr5 and tested cancer stem cell markers, Lgr5 does not seem to be a potential stemness marker or prognostic factor in pancreatic ductal adenocarcinoma. PMID: 25804119
    47. High LGR5 expression was associated with poor prognosis in patients with colorectal cancer(CRC) and that LGR5 is an efficient prognostic factor in CRC. [meta-analysis; review] PMID: 25192390
    48. we suggest that Musashi-1, Lgr5, and pEGFR are overexpressed in human SIAs and may play roles in human SIA carcinogenesis and progression. PMID: 25773390
    49. the higher expression level of DCLK1 in patients undergoing CRT can propose it as a more relevant candidate among CSC markers comparing to Lgr5 for colorectal cancer patients. PMID: 25631749
    50. increased expression of LGR5 during embryogenesis and the neonatal period alter skin development and homeostasis. PMID: 26003047

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 组织特异性：
    Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of th
  • 数据库链接：

    HGNC: 4504

    OMIM: 606667

    KEGG: hsa:8549

    STRING: 9606.ENSP00000266674

    UniGene: Hs.658889