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  4. ITGA6 Recombinant Monoclonal Antibody

ITGA6 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA172768A0HU
  • 规格：
    ￥1320
  • 图片：
    • Immunofluorescence staining of MCF-7 cell with CSB-RA172768A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 536-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Hela cells surface stained with CSB-RA172768A0HU (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • Uniprot No.：
    P23229
  • 基因名：
  • 别名：
    Integrin alpha-6 (CD49 antigen-like family member F) (VLA-6) (CD antigen CD49f) [Cleaved into: Integrin alpha-6 heavy chain, Integrin alpha-6 light chain, Processed integrin alpha-6 (Alpha6p)], ITGA6
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human ITGA6
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    4G10
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion. Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling.
  • 基因功能参考文献：
    1. RUNX1 regulates ITGA6 through a consensus RUNX1 binding motif in its promoter PMID: 28926098
    2. This study found a significant association between Hepatitis B virus (HBV)and integrin A6, which may be responsible for early migration and invasion of hepatocellular carcinoma (HCC). Thus, integrin A6 is a predictive marker for tumor recurrence and invasiveness of HBV-driven HCC PMID: 30010110
    3. alpha6-integrin is a matrix stiffness-regulated mechanosensitive molecule which confers an invasive fibroblast phenotype and mediates experimental lung fibrosis. PMID: 27535718
    4. N-glycosylation controlled the EGFR complex formation with integrin alpha5beta1 or alpha6beta4 PMID: 27641064
    5. ITGA6 might be involved in a mechanism that underlies radiation resistance and that ITGA6 could be a potential target for therapies aimed at overcoming radiation resistance in breast cancer. PMID: 27624978
    6. ITGA6 mRNA can be detected in stools of patients with colorectal cancers using droplet digital PCR. PMID: 28522907
    7. In particular, methylation levels of ITGA4, RERG, and ZNF671 could distinguish Nasopharyngeal carcinoma (NPC) patients from non-cancer nasopharyngeal epithelium (NNE) subjects. We identified the DNA methylation rates of previously unidentified NPC candidate genes. PMID: 28716111
    8. miR-92b/integrin alpha6/Akt axis controls the motility of esophageal squamous carcinoma cells. PMID: 28036265
    9. High ITGA6 expression is associated with colorectal cancer. PMID: 26895101
    10. During the past decades, many studies have provided evidence for a role of laminin-binding integrins in tumorigenesis, and both tumor-promoting and suppressive activities have been identified. (Review) PMID: 27562932
    11. alpha6 beta4 integrin promotes resistance of cancer cells to ferroptosis. PMID: 28972104
    12. Human pluripotent stem cells promote the expression of integrin alpha6beta1, and nuclear localization and inactivation of FAK to supports stem cell self-renewal. PMID: 26930028
    13. Results provide evidence that alpha3 and alpha6 integrins have significantly different internalization kinetics and that coordination exists between them for internalization in prostate cancer cells. PMID: 27509031
    14. IGF2 also requires integrin binding for signaling functions, and the IGF2 mutants that cannot bind to integrins act as antagonists of IGF1R. PMID: 28873464
    15. By directly binding to the 3'UTRs of ITGA6 and ITGB1, we found miR-30e-5p to suppress mainly the adhesion of ITGA6 in CRC cells to laminin.Taken together, miR-30e-5p is a novel effector of P53-induced suppression of migration, invasion and metastasis PMID: 28656629
    16. The SALL4 - integrin alpha6 - integrin beta1 network promotes cell migration for metastasis via activation of focal adhesion dynamics in basal-like breast cancer cells. PMID: 27773610
    17. FACS analysis of primary human Benign Prostatic Hyperplasia tissues confirms expansion of CD49f basal epithelia in patients with Basal cell hyperplasia. PMID: 28795417
    18. Increased integrin alpha6beta4 expression is associated with venous invasion and decreased overall survival in non-small cell lung cancers. PMID: 27107458
    19. alpha6beta4 integrin is a positive regulator of collective cell migration of A549 cells through influence on signal pathways in leader cells. PMID: 27922761
    20. The translational blocking of alpha5 and alpha6 integrin subunits affects migration and invasion, and increases sensitivity to carboplatin of SKOV-3 ovarian cancer cell line. PMID: 28131812
    21. alpha6(high)/CD71(low) cells give rise to a thicker pluristratified epithelium with lower seeding density and display a low Ki67 positive cells number, showing that they have reached the balance between proliferation and differentiation. PMID: 28134816
    22. The A380T Single Nucleotide Polymorphism of the ITGA6 gene was not associated with breast cancer development in the analyzed population of Misiones, Argentina. PMID: 27763564
    23. Positive Thy1 and CD49f expression is significantly associated with the progression and poor prognosis of chondrosarcoma. PMID: 27155928
    24. Ahr ligand aminoflavone targets breast cancer cells with stem cell-like properties, at least in part by abolishing alpha6-integrin expression. PMID: 26996297
    25. Platelet-enhanced endothelial colony forming cell angiogenesis requires platelet tetraspanin CD151 and alpha6beta1 integrin signaling. PMID: 26749288
    26. these findings identify CD151 and its interactions with integrins a3 and a6 as potential therapeutic targets for inhibiting stemness-driving mechanisms and stem cell populations in Glioblastoma. PMID: 26992919
    27. Schwann cells in the tumor-nerve environment affect tumor cell migration and invasion through regulation of the laminin receptor A6B1 and its variant A6pB1. PMID: 26239765
    28. HIF-dependent regulation of ITGA6 is one mechanism by which sorting for CD49f (+) cells enhances cancer stem cells. PMID: 27001172
    29. CD49f is a marker of bone marrow mesenchymal stem cells and is correlated with changes in these stem cells under inflammatory conditions. PMID: 26013602
    30. Genetic and Immunohistochemical Expression of Integrins ITGAV, ITGA6, and ITGA3 As Prognostic Factor for Colorectal Cancer PMID: 26674523
    31. We here identify CD49f and CD34 as markers to permit selection of the stem/progenitor cell-like population from human myometrium PMID: 26108791
    32. integrin alpha6beta4 stimulates invasion by promoting autocrine EGFR signaling through transcriptional up-regulation of key EGFR family members and by facilitating HGF-stimulated EGFR ligand secretion PMID: 26381405
    33. Amphiregulin enhances alpha6beta1 integrin expression and cell motility in human chondrosarcoma cells through Ras/Raf/MEK/ERK/AP-1 pathway. PMID: 25825984
    34. syndecans remain anchored to the alpha6beta4 integrin via its cytoplasmic domain, but the activation of cell motility is disrupted PMID: 26350464
    35. Unique sites of interaction between alphavbeta6 and uPAR are most likely located in uPAR domains II and III. PMID: 25318615
    36. our observations suggest that miR-25 is a key regulator of invasiveness in human prostate cancer through its direct interactions with av- and a6-integrin expression PMID: 25858144
    37. ITGA3, ITGA6, ITGB3,ITGB4 and ITGB5 are associated with GC susceptibility (rs2675), ITGA3, ITGA6, ITGB3, ITGB4 and ITGB5 are associated with gastric cancer susceptibility tumor stage and lymphatic metastasis in Chinese Han population. PMID: 25472585
    38. These results indicate that mAbs to the laminin a4 globular domain are able to inhibit tumor cell adhesion and migration on laminins 411 and 421, and that alpha6beta1 integrin and MCAM bind a4-laminins at very close sites on the globular domain PMID: 24681327
    39. Primary osteosarcoma patient tumor cell populations expressing high levels of CD49f and low levels of CD90 produce an aggressive xenograft tumor in mice. PMID: 24802970
    40. PIK3R3, ITGB1, ITGAL, and ITGA6, were involved in the regulation of actin cytoskeleton that link to triple-negative breast cancer migration. PMID: 24982892
    41. These data suggest that systemic targeting of the ITGA6-dependent function of established tumors in bone may offer a noncytotoxic approach to arrest the osteolytic progression of metastatic prostate cancer. PMID: 24739392
    42. Data indicate that integrin alpha6 repression by Kruppel-like factor-9 (KLF9) inhibits glioblastoma cell stemness and tumorigenicity. PMID: 25288800
    43. Inhibition of actin, FAK, and ILK expression resulted in significantly increased uPAR expression and the variant ITGA6p production in aggressive prostate and breast cancer cell lines. PMID: 25450398
    44. Integrin alpha6B-beta1 preferentially binds laminin 511 and this ligand-integrin ligation induces the TAZ nuclear localization and transcriptional activity. PMID: 25561492
    45. alpha6-integrin is required for hemangioma stem cellsadherence to laminin, vessel formation in vivo, and for homing to the liver. PMID: 24022922
    46. Role of N-terminal residues in Abeta interactions with integrin receptor and cell surface PMID: 24955499
    47. Necl-4 interacts in cis with integrin alpha6 beta4 through their extracellular regions and Necl-4 inhibited the phorbol ester-induced disassembly of hemidesmosomes PMID: 23611113
    48. NLGN1 and alpha6 integrin preferentially colocalize in the mature retinal vessels, whereas NLGN1 deletion causes an aberrant VE-cadherin, laminin and alpha6 integrin distribution in vessels PMID: 24860089
    49. Taken together, it was concluded that CD151 promotes the proliferation and migration of PC3 cells through the formation of CD151-integrin complex and the activation of phosphorylated ERK. PMID: 22684562
    50. The data presented herein clearly show that down regulation of ITGA6 gene in the human thymic epithelium triggers a complex cascade of effects upon the expression levels of several other cell migration-related genes. PMID: 24564203

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  • 相关疾病：
    Epidermolysis bullosa letalis, with pyloric atresia (EB-PA)
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor.
  • 蛋白家族：
    Integrin alpha chain family
  • 组织特异性：
    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limite
  • 数据库链接：

    HGNC: 6142

    OMIM: 147556

    KEGG: hsa:3655

    STRING: 9606.ENSP00000386896

    UniGene: Hs.133397