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  4. ACO1 Recombinant Monoclonal Antibody

ACO1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA298469A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate
      All lanes: ACO1 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 100 kDa
      Observed band size: 95-130 kDa
    • Immunofluorescence staining of HepG2 cell with CSB-RA298469A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 511-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing HepG2 cells stained with CSB-RA298469A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • Uniprot No.：
    P21399
  • 基因名：
    ACO1
  • 别名：
    Cytoplasmic aconitate hydratase (Aconitase) (EC 4.2.1.3) (Citrate hydro-lyase) (Ferritin repressor protein) (Iron regulatory protein 1) (IRP1) (Iron-responsive element-binding protein 1) (IRE-BP 1), ACO1, IREB1
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human ACO1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    22C10
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Bifunctional iron sensor that switches between 2 activities depending on iron availability. Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA.; Conversely, when cellular iron levels are high, binds a 4Fe-4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis-aconitate.
  • 基因功能参考文献：
    1. These results support the concept that IRP1 is an oxidative stress biosensor that mediates iron accumulation and cell death when deregulated by mitochondrial dysfunction. PMID: 28502703
    2. Results indicate that there is a dysregulation of DMT1 + IRE in IA testes, which might due to the up-regulation of IRP1 and HIF-1A. PMID: 28762519
    3. Evaluation of the iron regulatory protein-1 interactome has been presented. PMID: 29330752
    4. regulatory circuit involving FBXL5 and CIA acts through both IRPs to control iron metabolism and promote optimal cell growth PMID: 28768766
    5. Data suggest that Fe-S cluster of IRP1 plays role in sensing/regulating cellular iron homeostasis; IRP1 alternates between function as cytosolic aconitase [which contains Fe(4)-S(4) cluster in active-site cleft] and function as apoenzyme [which lacks Fe-S cluster] that binds to iron response element stem-loop structures present in several iron regulatory protein transcripts. [REVIEW] PMID: 28615439
    6. The SNP rs7874815 in the ACO1 gene was strongly associated with survival in pancreatic cancer. PMID: 26755275
    7. Our results confirm the observation that mitoNEET is important in transferring the iron sulfur clusters to the cytosolic aconitase in living cells and the His-87 ligand in mitoNEET plays important role in this process. PMID: 26778000
    8. Data show that IRP1 suppressed TfR1/DMT1 (+IRE) expression, limited the cellular iron content and decreased lactate dehydrogenase (LDH) release induced by hypoxia. PMID: 25727755
    9. These in vitro studies suggest that human GLRX3 is important for cytosolic Fe-S protein maturation. PMID: 26296460
    10. SIRT3 overexpression decreases TfR1 expression by inhibiting IRP1 and represses proliferation in pancreatic cancer cells. PMID: 24909164
    11. ACO1 gene expression was positively associated with adipogenic markers in subcutaneous and visceral adipose tissue. PMID: 25550467
    12. ACO1 genetic variation is associated with neuropathic pain and pain severity in HIV-infected patients on antiretroviral therapy PMID: 25144566
    13. MitoNEET governs a novel trafficking pathway to rebuild an Fe-S cluster into cytosolic aconitase/IRP1. PMID: 25012650
    14. Polymer-dependent translocation of IRP-1 in Cd(2+)-exposed cells may underlie effects of Cd(2+) on iron homeostasis. PMID: 25106854
    15. Data indicate that JTR-009, a benzimidazole, operated by preventing iron-regulatory protein-1 (IRP1) from binding to the iron-responsive element (IRE) in APP mRNA. PMID: 23935819
    16. Aco1 encodes a moonlighting protein that has aconitase activity as well as mRNA binding ability. PMID: 8041788
    17. Data indicate that silencing of Grx3 in HeLa cells decreases the activities of several cytosolic Fe/S proteins, including iron-regulatory protein 1, a major component of posttranscriptional iron regulation. PMID: 23615448
    18. Studies indicate that several genes have been linked to iron homeostasis, including transferrin (TF), iron regulatory protein 1 (ACO1) and transferrin receptor 2 (TFR2). PMID: 23817740
    19. Lead specifically induces dysregulation of IRP1 protein by activating the ERK1/2 signaling pathway indicating a novel role for IRP1 and the ERK/MAPK pathway in vascular endothelial functions. PMID: 22502979
    20. The RNA-binding and aconitase forms of IRP-1 can undergo interconversion dependent on the density of cells growing in culture. PMID: 22544036
    21. Ggenetic polymorphisms of the iron regulatory protein 1 and 2 genes may be associated with development of age-related macular degeneration. PMID: 22331484
    22. Molecular genetic analysis revealed point mutations within the FTL IRE. PMID: 22020773
    23. Dual-luciferase reporter assay showed that iron regulatory protein 1 (IRP)1 was directly downregulated by hypoxia-inducible factor 1/hypoxia response element system. PMID: 21360641
    24. Single nucleotide polymorphism in ACO1 gene is associated with cutaneous malignant melanoma. PMID: 20574843
    25. Selective translational control of the Alzheimer amyloid precursor protein transcript by iron regulatory protein-1. PMID: 20558735
    26. ATP/ADP binding maintains IRP-1 in a non-IRE-binding state. PMID: 20569198
    27. iron regulatory protein-1-mediated inhibition of hypoxia-inducible factor-2a translation is linked to the anti-inflammatory 15-deoxy-delta12,14-prostaglandin J2 PMID: 20354189
    28. gene coding and flanking regions were sequenced and examined for mutations that might modulate the iron burden of individuals harboring the common mutant hemochromatosis HFE genotype or cause hemochromatosis independent of mutations in the HFE gene PMID: 11783942
    29. Structural changes associated with switching activities of human iron regulatory protein 1 PMID: 11812787
    30. liver levels or this protein are down-regulated in hemochromatosis PMID: 12196178
    31. functional in the activation of iron uptake PMID: 12572667
    32. data suggest that the differential regulation of IRP1 and IRP2 during hypoxia may be important for cellular adaptation to low oxygen tension. PMID: 12855587
    33. A phosphomimetic mutation at Ser-138 renders IRP1 sensitive to iron-dependent degradation. PMID: 12972614
    34. Toxoplasma gondii infection resulted in increased activity in the iron response protein IRP1, which, in this state, stabilizes transferrin receptor mRNA from degradation. PMID: 15349772
    35. iron-replete HepG2 cells exhibit a narrow range of maximal responsiveness of the IRP-regulatory mechanism PMID: 15543932
    36. Apo-IRP1 exists as an equilibrium mixture of monomers and dimers in solution; however, only monomeric IRP1 is observed in complex with the iron regulatory element (IRE). PMID: 15938636
    37. Comparison of IRP1 in its aconitase form with known structures of homologous enzymes reveals well-conserved folds and active site environments with significantly different surface shapes and charge distributions. PMID: 16407072
    38. IRP1 controls iron homeostasis during cell proliferation, inflammation, and in response to diseases altering cytosolic Fe-S cluster assembly or disassembly. PMID: 16424901
    39. IRP1 activity level is higher in monocytes and macrophages of hereditary hemochrosis (HH) patients than in those of control subjects, and is increased in the duodenal samples of the patients with HH and iron-deficiency anemia. PMID: 16503547
    40. Overexpression of iron regulatory protein 1 suppresses growth of tumor xenografts PMID: 17127713
    41. Results describe thet effect of hypoxia on the binding and subcellular distribution of iron regulatory proteins 1 and 2. PMID: 17200797
    42. Thus, proper folding of human iron regulatory protein 1 strongly depends on its cellular environment, in contrast to other members of the aconitase family. PMID: 17244191
    43. Although the iron-responsive element does not itself hydrolyze ATP, its presence enhances iron regulatory protein-1's ATPase activity, and ATP hydrolysis results in loss of the complex in gel shift assays. PMID: 17521334
    44. analysis of the iron regulatory protein system PMID: 17604281
    45. Hypertransfusional (>8 transfusions/year) iron in liver biopsies correlated with increased expression (RNA) for iron regulatory proteins 1 and 2 (3-, 9- to 11-fold) and hepcidin RNA: (5- to 8-fold), while ferritin H and L RNA remained constant. PMID: 17613866
    46. Expression of TfR1 mRNA is increased when maternal iron deficiency progressed while there is no change in the expression of IRP1 mRNA in the placenta. PMID: 17877204
    47. Downregulated by HBX in human hepatoma cell lines. PMID: 18262302
    48. This study shows that expression of the transferrin receptor (TfR) is downregulated, whereas expression of the ferritin heavy chain is upregulated in Chlamydia trachomatis infected HeLa-229 cells. PMID: 19688112

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  • 亚细胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Aconitase/IPM isomerase family
  • 数据库链接：

    HGNC: 117

    OMIM: 100880

    KEGG: hsa:48

    STRING: 9606.ENSP00000309477

    UniGene: Hs.567229