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mutL Antibody

  • 中文名称：
    mutL兔多克隆抗体
  • 货号：
    CSB-PA543397HA01ENV
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in Recombinant protein
      All lanes: mutL antibody at 3μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      predicted band size: 68 kDa
      observed band size: 68 kDa
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Escherichia coli mutL Polyclonal antibody
  • Uniprot No.：
    P23367
  • 基因名：
    mutL
  • 别名：
    mutL antibody; b4170 antibody; JW4128 antibody; DNA mismatch repair protein MutL antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Escherichia coli
  • 免疫原：
    Recombinant Escherichia coli DNA mismatch repair protein MutL protein (1-615AA)
  • 免疫原种属：
    Escherichia coli
  • 标记方式：
    Non-conjugated

    本页面中的产品，mutL Antibody (CSB-PA543397HA01ENV)，的标记方式是Non-conjugated。对于mutL Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA543397HB01ENV mutL Antibody, HRP conjugated ELISA
    FITC CSB-PA543397HC01ENV mutL Antibody, FITC conjugated
    Biotin CSB-PA543397HD01ENV mutL Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of the final effector complex. The ATPase activity of MutL is stimulated by DNA.
  • 基因功能参考文献：
    1. In this review, we describe biochemical, biophysical and structural analyses that have clarified how MutL aids at discriminating the newly synthesized strand from its template and marking it for removal. [review] PMID: 25701376
    2. study found the deletion or insertion of a single LA repeat did not compromise the structural integrity of the protein, nor did it affect MutS- or DNA-binding activity;it severely compromised ATP binding and engagement of the N-terminal domains; both essential activities for proper DNA mismatch repair PMID: 23916559
    3. MutL accumulates from the mismatch site toward strand discrimination site along the DNA. PMID: 22241777
    4. Evidence for ATP-dependent structural rearrangement of nuclease catalytic site in DNA mismatch repair endonuclease MutL PMID: 21953455
    5. C-terminal domain of MutL encompasses a bona fide DNA polymerase III beta-binding motif that mediates a weak, yet specific, interaction between the two proteins. PMID: 21050827
    6. The presence of phosphate minimizes further MutL oligomerization beyond a dimer PMID: 21793594
    7. Studies indicate that a stable MutL-ssDNA interaction is unlikely to occur at physiological salt. PMID: 21103398
    8. MutL homologues play a key role in determining biologic outcome by recruiting and/or activating effector proteins in response to lesion recognition by MutS. PMID: 19953589
    9. Data report the crystal structure of the Escherichia coli MutL C-terminal dimerization domain and the likelihood of its conservation among MutL homologs. PMID: 15470502
    10. revised model for the biological dimer, which has important implications for understanding the heterodimerization of eukaryotic MutL homologues, modeling the MutL holoenzyme and predicting protein-protein interaction sites PMID: 16024043
    11. MutL determines effective DNA homology in recombination processes PMID: 17502621
    12. An increase in mutL gene copies was also able to partially compensate the hypermutator phenotype of a mutS-defective E. coli derivative. PMID: 17825069
    13. Results suggest a mobile MutS-MutL complex in DNA mismatch repair signalling, that leaves the DNA mismatch prior to, or at the time of, activation of downstream effector molecules such as Vsr or MutH. PMID: 19474347

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  • 蛋白家族：
    DNA mismatch repair MutL/HexB family
  • 数据库链接：

    KEGG: ecj:JW4128

    STRING: 316385.ECDH10B_4365