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  4. Recombinant Escherichia coli DNA mismatch repair protein MutL (mutL)

Recombinant Escherichia coli DNA mismatch repair protein MutL (mutL)

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  • 中文名称：
    大肠杆菌mutL重组蛋白
  • 货号：
    CSB-EP543397ENV
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    mutL
  • Uniprot No.：
  • 别名：
    mutL; b4170; JW4128; DNA mismatch repair protein MutL
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    71.9kDa
  • 表达区域：
    1-615aa
  • 氨基酸序列
    MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDIDIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASISSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKFLRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGTAFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQACEDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQLETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPNAQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLERDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALAELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLMSEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of the final effector complex. The ATPase activity of MutL is stimulated by DNA.
  • 基因功能参考文献：
    1. In this review, we describe biochemical, biophysical and structural analyses that have clarified how MutL aids at discriminating the newly synthesized strand from its template and marking it for removal. [review] PMID: 25701376
    2. study found the deletion or insertion of a single LA repeat did not compromise the structural integrity of the protein, nor did it affect MutS- or DNA-binding activity;it severely compromised ATP binding and engagement of the N-terminal domains; both essential activities for proper DNA mismatch repair PMID: 23916559
    3. MutL accumulates from the mismatch site toward strand discrimination site along the DNA. PMID: 22241777
    4. Evidence for ATP-dependent structural rearrangement of nuclease catalytic site in DNA mismatch repair endonuclease MutL PMID: 21953455
    5. C-terminal domain of MutL encompasses a bona fide DNA polymerase III beta-binding motif that mediates a weak, yet specific, interaction between the two proteins. PMID: 21050827
    6. The presence of phosphate minimizes further MutL oligomerization beyond a dimer PMID: 21793594
    7. Studies indicate that a stable MutL-ssDNA interaction is unlikely to occur at physiological salt. PMID: 21103398
    8. MutL homologues play a key role in determining biologic outcome by recruiting and/or activating effector proteins in response to lesion recognition by MutS. PMID: 19953589
    9. Data report the crystal structure of the Escherichia coli MutL C-terminal dimerization domain and the likelihood of its conservation among MutL homologs. PMID: 15470502
    10. revised model for the biological dimer, which has important implications for understanding the heterodimerization of eukaryotic MutL homologues, modeling the MutL holoenzyme and predicting protein-protein interaction sites PMID: 16024043
    11. MutL determines effective DNA homology in recombination processes PMID: 17502621
    12. An increase in mutL gene copies was also able to partially compensate the hypermutator phenotype of a mutS-defective E. coli derivative. PMID: 17825069
    13. Results suggest a mobile MutS-MutL complex in DNA mismatch repair signalling, that leaves the DNA mismatch prior to, or at the time of, activation of downstream effector molecules such as Vsr or MutH. PMID: 19474347

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  • 蛋白家族：
    DNA mismatch repair MutL/HexB family
  • 数据库链接：

    KEGG: ecj:JW4128

    STRING: 316385.ECDH10B_4365