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ZKSCAN3 Antibody

  • 货号：
    CSB-PA861122LA01HU
  • 规格：
    ￥440
  • 促销：
    小规格抗体限时一口价
  • 图片：
    • Western Blot
      Positive WB detected in: THP-1 whole cell lysate, HL-60 whole cell lysate
      All lanes: ZKSCAN3 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 61, 44 kDa
      Observed band size: 44 kDa
    • IHC image of CSB-PA861122LA01HU diluted at 1:200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela cells with CSB-PA861122LA01HU at 1:200, counter-stained with DAPI.The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 其他：

产品详情

  • 产品名称：
    Rabbit anti-Homo sapiens (Human) ZKSCAN3 Polyclonal antibody
  • Uniprot No.：
    Q9BRR0
  • 基因名：
    ZKSCAN3
  • 别名：
    dJ874C20.1 antibody; mszf35 antibody; SCAN-KRAB-zinc finger protein antibody; Skz1 antibody; Zf47 antibody; Zfp-47 antibody; ZFP306 antibody; Zfp307 antibody; Zfp47 antibody; Zinc finger and SCAN domain containing protein 13 antibody; Zinc finger and SCAN domain-containing protein 13 antibody; Zinc finger protein 306 antibody; Zinc finger protein 306; isoform CRA_a antibody; Zinc finger protein 307 antibody; Zinc finger protein 309 antibody; Zinc finger protein 47 homolog antibody; Zinc finger protein with KRAB and SCAN domains 3 antibody; Zinc finger protein with KRAB and SCAN domains 3 antibody; Zinc finger protein zfp47 antibody; Zinc finger with KRAB and SCAN domains 3 antibody; ZKSC3_HUMAN antibody; ZKSCAN3 antibody; ZNF309 antibody; ZSCAN13 antibody; ZSCAN35 antibody
  • 宿主：
    Rabbit
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human Zinc finger protein with KRAB and SCAN domains 3 protein (8-275AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated

    本页面中的产品，ZKSCAN3 Antibody (CSB-PA861122LA01HU)，的标记方式是Non-conjugated。对于ZKSCAN3 Antibody，我们还提供其他标记。见下表：

    可提供标记
    标记方式 货号 产品名称 应用
    HRP CSB-PA861122LB01HU ZKSCAN3 Antibody, HRP conjugated ELISA
    FITC CSB-PA861122LC01HU ZKSCAN3 Antibody, FITC conjugated
    Biotin CSB-PA861122LD01HU ZKSCAN3 Antibody, Biotin conjugated ELISA
  • 克隆类型：
    Polyclonal
  • 抗体亚型：
    IgG
  • 纯化方式：
    >95%, Protein G purified
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:20-1:200
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters. Also acts as a transcription activator and promotes cancer cell progression and/or migration in various tumors and myelomas.
  • 基因功能参考文献：
    1. These results suggest that ZKSCAN3 plays an important role in bladder cancer outgrowth PMID: 27447553
    2. ZKSCAN3 appears to promote colorectal tumor progression and invasion. ZKSCAN3 may facilitate hepatic metastasis of CRC associated with CEA particularly in cases with CEA-producing tumor PMID: 27127149
    3. Suggest ZKSCAN3 as an autophagy master switch regulating the expression of a transcriptional network of genes integral to autophagy and lysosome biogenesis/function. PMID: 23434374
    4. ZKSCAN3 induction represents a mechanism by which myeloma cells can induce cyclin D2 dysregulation and contribute to disease pathogenesis. PMID: 21057542
    5. ZKSCAN3 adds to an expanding set of encoded products contributing to the progression of colorectal cancer PMID: 18519692
    6. ZKSCAN3 regulates the expression of several genes favoring tumor progression including integrin beta 4 and VEGF PMID: 18940803

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Mainly localizes in the nucleus. Under starvation conditions translocates to the cytoplasm, allowing expression of target genes involved in autophagy and lysosome biogenesis/function.
  • 蛋白家族：
    Krueppel C2H2-type zinc-finger protein family
  • 数据库链接：

    HGNC: 13853

    OMIM: 612791

    KEGG: hsa:80317

    STRING: 9606.ENSP00000252211

    UniGene: Hs.380930